Purification and partial characterization of an antimicrobial peptide produced by Bacillus sp. strain P45, a bacterium from the Amazon basin fish Piaractus mesopotamicus

Sirtori, Lisana Reginini; Cladera-Olivera, Florencia; Lorenzini, Daniel M.; Tsai, Siu Mui; Brandelli, Adriano

doi:10.2323/jgam.52.357

Cited by 26 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently bacteriocin-like peptides produced by bacteria isolated from Amazonian fishes have been described [15,21]. Among those bacteria, the strain P45 produces a surfactin-like peptide that was previously purified and characterized [22]. In this work, the effect and the mode of action of BLS P45 on indicator bacteria were addressed, particularly using L. monocytogenes as the target organism.…”

Section: Discussionmentioning

confidence: 99%

“…P45, isolated from the fish Piaractus mesopotamicus, was used for peptide P45 production [22]. The indicator strain was Listeria monocytogenes ATCC 7644 (American Type Culture Collection, Rockville, USA).…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…This culture supernatant was used to evaluate the inhibitory effect on growth of L. monocytogenes. Antimicrobial activity was further purified by precipitation with ammonium sulfate and gel filtration chromatography as described elsewhere [22]. Alternativelly, the column was eluted with 10 mmol l -1 phosphate buffer pH 7.0 containing 1.5 mol l -1 NaCl.…”

Section: Production Of Antimicrobial Substancementioning

confidence: 99%

“…isolated from the intestines of the teleost fish Piaractus mesopotamicus of the Brazilian Amazon basin. This strain produces an antimicrobial peptide that was purified and characterized as a surfactin-like peptide [22]. The aim of this work was to investigate the effect and the mode of action of the antimicrobial peptide P45, and to evaluate its potential use as antimicrobial to prevent the proliferation of L. monocytogenes.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Mode of action of antimicrobial peptide P45 on Listeria monocytogenes

Sirtori

Motta

Brandelli

2008

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

The mode of action of an antimicrobial peptide produced by Bacillus sp. P45 isolated from the intestine of the Amazonian basin fish Piaractus mesopotamicus was investigated. The antimicrobial peptide was purified from culture supernatants by precipitation with ammonium sulfate and gel filtration chromatography. The peptide has an EC(50) of 300 AU (activity units) ml(-1) and kills all viable cells of Listeria monocytogenes with a concentration of 800 AU ml(-1). A decrease in OD was observed when L. monocytogenes cultures were treated with the peptide, suggesting that cells were lysed. Transmission electron microscopy showed damage of the cell envelope and loss of protoplasmic material. The peptide P45 was bactericidal and bacteriolytic to L. monocytogenes. There is evidence that the mode of action is interfering at cell membranes and the cell wall. The knowledge of the mode of action of antimicrobial peptides is an essential step to consider their utilization in food or clinic.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Bacterial Strainsmentioning

confidence: 99%

Section: Production Of Antimicrobial Substancementioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Mode of action of antimicrobial peptide P45 on Listeria monocytogenes

Sirtori

Motta

Brandelli

2008

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…10). Sirtori et al (2006) reported clear absorption peaks at 3,500, 2,925, 1,639, and 1,546 cm −1 corresponding to the O-H, C-H, C-N and angular deformation of the N-H bond. Kong and Yu (2007) also detected bands at peaks of 3100, 1600-1690, 1480-1575 and 1229-1301 cm −1 that are assigned to N-H, C5O, C-N and N-H. Kumar et al (2010) reported absorption bands at 1670, 1539, 1418, and 1488 cm −1 attributing to N-H, C5O, O-H and CO.…”

Section: Microbiologymentioning

confidence: 99%

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Zada¹,

Naseem²,

Lee³

et al. 2016

JCKS

View full text Add to dashboard Cite

Bacterial strains having the ability to inhibit the growth of other bacteria were isolated from soil samples collected from Kashmir Smast (smast is Pushto for cave), Khyber Pakhtunkhwa, Pakistan. The study includes mineralogical and geochemical analyses of soil sample collected from the cave, so as to describe the habitat from which the microorganisms have been isolated. Total bacterial count of the soil sample was 5.25 3 10 4 CFU mL −1. Four bacterial isolates having activity against test organisms Micrococcus luteus, Klebsiella sp., Pseudomonas sp., and Staphylococcus aureus were screened out for further study. Two of the isolates were found to be Gram-positive and the other two Gram-negative. The four isolates showing antibacterial activity were identified as Serratia sp. KC1-MRL, Bacillus licheniformis KC2-MRL, Bacillus sp. KC3-MRL, and Stenotrophomonas sp. KC4-MRL on the basis of 16S rRNA sequence analysis. Although all isolates showed antibacterial activity, only Bacillus licheniformis KC2-MRL was selected for further study due to its large zone of inhibition. Antibacterial activity of B. licheniformis KC2-MRL was optimum when grown in nutrient broth adjusted to pH 5 and after 24 hours of incubation at 35 uC. The extracted antibacterial compound was stable at pH 5-7 and 40 uC when incubated for 1 hour. The strain was found resistant against cefotaxime (ctx). Atomic-absorption analysis of the soil sample collected from the cave showed high concentrations of calcium (332.938 mg kg ) compared to the control soil collected outside the cave. FTIR spectrum of the concentrated protein showed similarity to bacitracin. The antibacterial compound showed activity against both Gram-negative and positive test strains. Mineralogy of Kashmir Smast is diverse and noteworthy. Different geochemical classes identified by X-ray diffraction were nitrates, oxides, phosphates, silicates, and sulfates. Weathered cave limestone contributes notably to the formation of these minerals or compounds. FTIR spectroscopic analysis helped to identify minerals such as quartz, clinochlore, vermiculite, illite, calcite, and biotite.

show abstract

Antimicrobial Peptides and their Potential as Therapeutants in Aquaculture

Douglas¹

2011

Aquaculture Biotechnology

View full text Add to dashboard Cite

Purification and partial characterization of an antimicrobial peptide produced by Bacillus sp. strain P45, a bacterium from the Amazon basin fish Piaractus mesopotamicus

Cited by 26 publications

References 30 publications

Mode of action of antimicrobial peptide P45 on Listeria monocytogenes

Mode of action of antimicrobial peptide P45 on Listeria monocytogenes

Geochemical and mineralogical analysis of Kashmir Cave (SMAST), Buner, Pakistan, and isolation and characterization of bacteria having antibacterial activity

Antimicrobial Peptides and their Potential as Therapeutants in Aquaculture

Contact Info

Product

Resources

About