Mode of action of antimicrobial peptide P45 on <i>Listeria monocytogenes</i>

Sirtori, Lisana Reginini; Motta, Amanda de Souza da; Brandelli, Adriano

doi:10.1002/jobm.200700406

Cited by 16 publications

(12 citation statements)

References 49 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sensitive Grampositive bacteria include L. monocytogenes, S. aureus and clinical isolates of Streptococcus sp., while Gram-negative bacteria such as A. hydrophila and clinical isolates of Salmonella Gallinarium and P. haemolytica, were also inhibited by this peptide. Peptide P45 has bactericidal and bacteriolytic activity against L. monocytogenes (Sirtori et al, 2008). The physicochemical properties of P45 (1450 Da) are typical of small antimicrobial peptides, being sensitive to proteolytic enzymes (proteinase K, pronase E, trypsin and papain) and stable over a wide pH range of 3-11.…”

Section: Other Bacillus Sppmentioning

confidence: 99%

“…However, the peptide was not very heat stable, losing activity at 80 1C for 30 min, with 70% residual activity remaining, at 100 1C with 35% residual activity remaining and activity being completely lost at 121 1C for 20 min (Sirtori et al, 2006). Peptide P45 has bactericidal and bacteriolytic activity against L. monocytogenes (Sirtori et al, 2008).…”

Section: Other Bacillus Sppmentioning

confidence: 99%

See 1 more Smart Citation

Diversity and applications ofBacillusbacteriocins

et al. 2011

View full text Add to dashboard Cite

Members of the genus Bacillus are known to produce a wide arsenal of antimicrobial substances, including peptide and lipopeptide antibiotics, and bacteriocins. Many of the Bacillus bacteriocins belong to the lantibiotics, a category of post-translationally modified peptides widely disseminated among different bacterial clades. Lantibiotics are among the best-characterized antimicrobial peptides at the levels of peptide structure, genetic determinants and biosynthesis mechanisms. Members of the genus Bacillus also produce many other nonmodified bacteriocins, some of which resemble the pediocin-like bacteriocins of the lactic acid bacteria (LAB), while others show completely novel peptide sequences. Bacillus bacteriocins are increasingly becoming more important due to their sometimes broader spectra of inhibition (as compared with most LAB bacteriocins), which may include Gram-negative bacteria, yeasts or fungi, in addition to Gram-positive species, some of which are known to be pathogenic to humans and/or animals. The present review provides a general overview of Bacillus bacteriocins, including primary structure, biochemical and genetic characterization, classification and potential applications in food preservation as natural preservatives and in human and animal health as alternatives to conventional antibiotics. Furthermore, it addresses their environmental applications, such as bioprotection against the pre- and post-harvest decay of vegetables, or as plant growth promoters.

show abstract

Section: Other Bacillus Sppmentioning

confidence: 99%

Section: Other Bacillus Sppmentioning

confidence: 99%

Diversity and applications ofBacillusbacteriocins

et al. 2011

View full text Add to dashboard Cite

show abstract

“…They produce a large number of antimicrobial metabolites and bio-peptides with different chemical structures and diversity, such as bacteriocins, bacteriocin-like substances and lipopeptides [3]. Although most of these substances are active against Gram-positive bacteria, some of them have a wide range of bio-activity towards Gram-negative bacteria and filamentous fungi [4]. Among antifungal compounds, lipopeptides identified to play a major role in disease suppression [5].…”

Section: Introductionmentioning

confidence: 99%

In-vitro antibacterial, antifungal, antioxidant and functional properties of Bacillus amyloliquefaciens

Kadaikunnan

Rejiniemon²,

Khaled

et al. 2015

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

BackgroundFood born pathogenic bacteria and filamentous fungi are able to grow on most foods, including natural foods, processed foods, and fermented foods and create considerable economic loss. The aim of this study was to determine the antibacterial, antifungal, antioxidant and functional properties of Bacillus amyloliquefaciens recovered from silage.MethodsMinimum Inhibitory Concentration (MIC) of the compounds was assessed by using broth micro dilution method. The 1,1-diphenyl–2-picrylhydrazyl (DPPH)-radical scavenging and hydroxyl radical-scavenging abilities were measured to evaluate antioxidant activity of the strain.ResultsPrimary antimicrobial compound production screening revealed that B. amyloliquefaciens exhibited significant activity against all the tested bacteria and fungi compared to other strains. The 16S rRNA and gyrase A gene sequence analysis determined using molecular biological tools confirmed that the strain was 99% similarity towards B. amyloliquefaciens. The Minimum Inhibitory Concentration (MIC) of ethyl acetate extract against Bacillus subtilis, Enterococcus cloacae and Staphylococcus aureus were 25.0 μg ml−1, and S, epidermidis were 12.5 μg ml−1, respectively. Filamentous fungi Aspergillus clavatus, A. fumigates, A. niger and Gibberella moniliformis showed 25 μg ml−1. VJ-1 was able to survive the gastrointestinal conditions simulating the stomach and duodenum passage with the highest percentage of hydrophobicity. In addition, its resistance to hydrogen peroxide and highest hydroxyl radical and 2, 2-diphenyl–1-picrylhydrazyl (DPPH) scavenging activities, with inhibition rates of 56.84% and 67.12% respectively, were its advantage. An antimicrobial susceptibility pattern was an intrinsic feature of this strain, and thus, consumption does not represent a health risk to humans.ConclusionBacillus amyloliquefaciens might be a promising candidate for new pharmaceutical agents and probiotics.

show abstract

“…Changes in the turbidity at 600 nm and viable cells count (cfu/ml) were measured at zero time and after 10, 30, 60, and 120 min of incubation. Viable cells count was determined on nutrient agar plates [26].…”

Section: Mode Of Bacteriocin Actionmentioning

confidence: 99%

Detection, purification and characterization of a bacteriocin produced by Bacillus subtilis NK16 exhibits a significant antimicrobial activity against clinical Staphylococcus spp.

Journal¹

2017

Baghdad Sci.J

View full text Add to dashboard Cite

Bacteriocin is an important antimicrobial peptide that can be used in industrial and medical fields due to its characteristics of antibacterial, food preservation and anticancer activities. Fifty isolates of Bacillus sp were collected from different soil samples which were already recognized via morphological and biochemical identification process. The isolates were screened for bacteriocin production effective against Staphylococcus spp in order to select the highest producing isolate. The isolate NK16 showed the maximum bacteriocin production (80 AU/ml) which was further characterized as Bacillus subtilis NK 16 through using API identification system (API 20E and API 50CHB). Then, next step was to detect the optimal conditions for maximum bacteriocin production which were found to be brain-heart infusion broth as the best production medium with pH 6, 30oCand 2% inoculum size. Bacteriocin was partially purified by precipitation with ammonium sulphate and then separation with sephadex G-150 gel filtration. The specific activity of the resulted partial purified bacteriocin was increased to 853.33 AU/mg with 38 fold purification and 24% yield. The study of bacteriocin characterization revealed that the activity of bacteriocin was stable after 10 min at 20, 30, 40oC whereas 50% of the bacteriocin activity was lost after exposure to 50oC and decreased to approximately 20 AU/ml at 60,70 and 80 Co. In addition, bacteriocin activity showed stability at pH 6 and 7 for 30 min while it was decreased by approximately 50% at pH 5 and 8, and completely inhibited at pH 4 and 9. On the other hand, the investigation of mode of action showed that bacteriocin has a bactericidal activity. Antimicrobial activity tests of the partial purified bacteriocin displayed a significant activity against most clinical Staphylococcus aureus and Staphylococcus epidermidis isolates, whereas it was less effective against Staphylococcus saprophyticus isolates.

show abstract

Mode of action of antimicrobial peptide P45 on Listeria monocytogenes

Cited by 16 publications

References 49 publications

Diversity and applications ofBacillusbacteriocins

Diversity and applications ofBacillusbacteriocins

In-vitro antibacterial, antifungal, antioxidant and functional properties of Bacillus amyloliquefaciens

Detection, purification and characterization of a bacteriocin produced by Bacillus subtilis NK16 exhibits a significant antimicrobial activity against clinical Staphylococcus spp.

Contact Info

Product

Resources

About