Purification and kinetics of sheep kidney cortex glucose-6-phosphate dehydrogenase

Kinetic mechanism and some properties of glucose-6-phosphate dehydrogenase from sheep brain cortex

“…factors such as; dilution of the enzyme sample, ionic factors (salt) or physical effects. The molecular weight of the G6PD from sheep brain cortex was in accordance with our previous studies [6][7][8].…”

“…In our previous study we have found that sheep kidney cortex G6PD was to operate according to a Ping Pong Bi Bi mechanism. In the same study we have suggested that the kinetic mechanism was changing by the age [8]. Human erythrocyte G6PD was shown to follow either a random or a Theorell-Chance mechanism [33].…”

“…We have previously described a purification protocol for G6PD from various tissues; bovine lens, sheep and lamb kidney cortex [6][7][8]. In this study, we have determined the kinetic mechanism and few properties of the G6PD which was purified from sheep brain cortex [5].…”

“…G6PD was purified as previously described (5)(6)(7)(8). The method included 2', 5'-ADP-Sepharose 4B affinity chromatography and DEAE Sepharose Fast Flow ion exchange chromatographies after ultracentrifugation.…”

In vitroeffects of rosmarinic acid on glutathione reductase and glucose 6-phosphate dehydrogenase

“…We have previously described a purification method for G6PD from bovine lens and sheep kidney cortex (Ulusu et al, 1999;Ulusu & Tandogan, 2006). We have used the same method for the purification of G6PD from sheep brain cortex.…”