volume 51, issue 1, P27-40 2007
DOI: 10.1002/jctb.280510103
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Abstract: Abstract A mutant strain with high penicillin G acylase activity was derived from Escherichia coli ATCC 11105 by chemical mutagenesis, using N‐methyl‐N′‐nitro‐N‐nitrosoguanidine. Penicillin acylase was extracted from the mutant strain and highly purified by DEAE‐cellulose and hydroxyapatite column chromatography followed by preliminary precipitation steps. Vm and Km values of the enzyme (specific activity: 24·81 U mg−1, protein concentration: 0.56 mg cm−3) were found to be 22.73 U cm−3 mm−1 and 3.18 mmold m−3…

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