Purification and identification of antioxidant peptides from duck plasma proteins

Yang, Jing; Huang, Jichao; Dong, Xiufang; Zhang, Yali; Zhou, Xinghu; Huang, Ming; Zhou, Guanghong

doi:10.1016/j.foodchem.2020.126534

Cited by 76 publications

(61 citation statements)

References 38 publications

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“…Therefore, the hydrophobic amino acids were critical for their protective response in lipid peroxidation by eliminating the free radicals derived from lipids in a heterogeneous lipid phase [ 64 ]. Gly residue can maintain the strong flexibility of the polypeptide skeleton and act as single hydrogen donor to neutralize ROS [ 25 , 65 ]. Hydrophilic amino acids including Glu, Gln, and Lys in EVGK and RCLQ have a positive influence on their Fe 2+ chelating ability [ 65 ].…”

Section: Resultsmentioning

confidence: 99%

“…Gly residue can maintain the strong flexibility of the polypeptide skeleton and act as single hydrogen donor to neutralize ROS [ 25 , 65 ]. Hydrophilic amino acids including Glu, Gln, and Lys in EVGK and RCLQ have a positive influence on their Fe 2+ chelating ability [ 65 ]. Asp, Glu, and Gln were found to show remarkable influence on the antioxidant abilities of NYDGSTDYGILQINSR and LDEPDPLI [ 66 , 67 ].…”

Section: Resultsmentioning

confidence: 99%

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Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

et al. 2021

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For utilizing the largest source of marine proteins, Antarctic krill (Euphausia superba) proteins were defatted and hydrolyzed separately using pepsin, alcalase, papain, trypsin, and netrase, and alcalase hydrolysate (EPAH) showed the highest DPPH radical (DPPH·) and hydroxyl radical (HO·) scavenging activity among five hydrolysates. Using ultrafiltration and chromatography methods, fifteen antioxidant peptides were purified from EPAH and identified as Asn-Gln-Met (NQM), Trp-Phe-Pro-Met (WFPM), Gln-Asn-Pro-Thr (QNPT), Tyr-Met-Asn-Phe (YMNF), Ser-Gly-Pro-Ala (SGPA), Ser-Leu-Pro-Tyr (SLPY), Gln-Tyr-Pro-Pro-Met-Gln-Tyr (QYPPMQY), Glu-Tyr-Glu-Ala (EYEA), Asn-Trp-Asp-Asp-Met-Arg-Ile-Val-Ala-Val (NWDDMRIVAV), Trp-Asp-Asp-Met-Glu-Arg-Leu-Val-Met-Ile (WDDMERLVMI), Asn-Trp-Asp-Asp-Met-Glu-Pro-Ser-Phe (NWD-DMEPSF), Asn-Gly-Pro-Asp-Pro-Arg-Pro-Ser-Gln-Gln (NGPDPRPSQQ), Ala-Phe-Leu-Trp-Asn (AFLWA), Asn-Val-Pro-Asp-Met (NVPDM), and Thr-Phe-Pro-Ile-Tyr-Asp-Tyr-Pro-Gln (TFPIYDPQ), respectively, using a protein sequencer and ESI/MS. Among fifteen antioxidant peptides, SLPY, QYPPMQY and EYEA showed the highest scavenging activities on DPPH· (EC50 values of 1.18 ± 0.036, 1.547 ± 0.150, and 1.372 ± 0.274 mg/mL, respectively), HO· (EC50 values of 0.826 ± 0.027, 1.022 ± 0.058, and 0.946 ± 0.011 mg/mL, respectively), and superoxide anion radical (EC50 values of 0.789 ± 0.079, 0.913 ± 0.007, and 0.793 ± 0.056 mg/mL, respectively). Moreover, SLPY, QYPPMQY and EYEA showed strong reducing power, protective capability against H2O2-damaged plasmid DNA, and lipid peroxidation inhibition ability. Furthermore, SLPY, QYPPMQY, and EYEA had high stability under temperatures lower than 80 °C, pH values ranged from 6–8, and simulated GI digestion for 180 min. The results showed that fifteen antioxidant peptides from alcalase hydrolysate of Antarctic krill proteins, especially SLPY, QYPPMQY and EYEA, might serve as effective antioxidant agents applied in food and health products.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

et al. 2021

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“…The authors indicate that sulfur-containing amino acids (C, M) as well as steric and electrostatic factors determine the antioxidant activity of SYPTECRMR. The presence of C, R, E, Q, G, L, and A residues in the structure of RCLQ and EVGK peptides obtained from duck plasma proteins may explain their high antioxidant activities [ 89 ]. Wu et al reported that the high antioxidant potency of QMDDQ from a shrimp protein hydrolysate might be attributed to the greater number of active hydrogen sites and functional groups.…”

Section: Antioxidant Peptidesmentioning

confidence: 99%

“…Zhang et al [ 92 ] concluded that valine at the N-terminus of VYLPR might be helpful to enhance the antioxidant activity of this peptide. Yang et al [ 89 ] showed that the crucial antiradical activity against ABTS +• of a novel peptide ATVY was dependent on Tyr positions in the N-terminus. In turn, Sheng et al [ 93 ] reported that the presence of antioxidant amino acids (W, Y, and M) did not determine the cellular antioxidative effect of peptides.…”

Section: Antioxidant Peptidesmentioning

confidence: 99%

“…Hydrophobic amino acids play a major role in scavenging free radicals, whereas polar amino acids are responsible for reducing and chelating metal ions [ 85 ]. Seven novel peptides—LDGP, TGVGTK, EVGK, RCLQ, LHDVK, KLGA, and AGGVPAG—with antioxidant activities were obtained from duck plasma proteins, identified, and synthesized by Yang et al [ 89 ]. EVGK exhibited the highest Fe 2+ chelating ability, whereas RCLQ had the highest reducing power and ABTS +• and DPPH • scavenging activity.…”

Section: Antioxidant Peptidesmentioning

confidence: 99%

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Current Trends of Bioactive Peptides—New Sources and Therapeutic Effect

Jakubczyk

Karaś

Rybczyńska‐Tkaczyk

et al. 2020

Foods

142

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Generally, bioactive peptides are natural compounds of food or part of protein that are inactive in the precursor molecule. However, they may be active after hydrolysis and can be transported to the active site. Biologically active peptides can also be synthesized chemically and characterized. Peptides have many properties, including antihypertensive, antioxidant, antimicrobial, anticoagulant, and chelating effects. They are also responsible for the taste of food or for the inhibition of enzymes involved in the development of diseases. The scientific literature has described many peptides with bioactive properties obtained from different sources. Information about the structure, origin, and properties of peptides can also be found in many databases. This review will describe peptides inhibiting the development of current diseases, peptides with antimicrobial properties, and new alternative sources of peptides based on the current knowledge and documentation of their bioactivity. All these issues are part of modern research on peptides and their use in current health or technological problems in food production.

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Octopus‐derived antioxidant peptide protects against hydrogen peroxide‐induced oxidative stress in IEC‐6 cells

Peng

Cai

Pan

2022

Food Science & Nutrition

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This study aims to find antioxidant peptides from octopus protein hydrolyzates and verify the protective effects against H2O2‐induced oxidative stress in IEC‐6 cells. After the alcalase hydrolysate was ultrafiltrated, purified by Sephadex G‐25 gel fractionation and semipreparative reversed‐phase high‐performance liquid chromatography (RP‐HPLC), 16 peptides were identified, and chemically synthesized. In particular, the peptides AQNY, AMMLAW, FEGAW, GGAW, VDTVVCVW, and VVCLW showed better oxygen radical absorbance capacity (ORAC) and ABTS radical scavenging capacity. Among them, the smallest‐molecular‐weight peptide GGAW exhibited the best antioxidant activity. Furthermore, GGAW protected IEC‐6 cells from H2O2‐induced oxidative damage by significantly reducing the generation of reactive oxygen species (ROS), malondialdehyde (MDA), and lactate dehydrogenase (LDH), and increasing the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH‐PX), thereby improving cell viability. These results indicated that the peptide GGAW possessed the antioxidant capacity to prevent oxidative stress damage.

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Purification and identification of antioxidant peptides from duck plasma proteins

Cited by 76 publications

References 38 publications

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

Purification, Identification, Activity Evaluation, and Stability of Antioxidant Peptides from Alcalase Hydrolysate of Antarctic Krill (Euphausia superba) Proteins

Current Trends of Bioactive Peptides—New Sources and Therapeutic Effect

Octopus‐derived antioxidant peptide protects against hydrogen peroxide‐induced oxidative stress in IEC‐6 cells

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