Purification and identification of anti-obesity peptides derived from Spirulina platensis

Fan, Xiaodan; Cui, Yujiao; Zhang, Ruilin; Zhang, Xuewu

doi:10.1016/j.jff.2018.05.066

Cited by 69 publications

(41 citation statements)

References 19 publications

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“…As examples, peptide fractions of vacuum packaging string bean ( Phaseolus Vulgaris L.) showed low IC 50 values (0.05 mg/mL), highlighting an improvement with peptide fraction < 3.5 kDa (IC 50 = 0.008 mg/mL) [ 39 ]. Similarly, protein hydrolysates from Spirulina platensis , specifically the 3–5 kDa fraction, show 72% inhibition of PL [ 40 ]. Chemically synthesized peptides (at concentration of 20 mg/mL) from cumin seed showed the best PL inhibition, with 54.6% for peptide FFRSKLLSRGAAAAKGALLPQYW, 50.1% for peptide RCMAFLLSDGAAAAQQLLPQYW, and 22.6% for peptide RPAQPNYPWTAVLVFRH [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Cocoa (Theobroma cacao L.) Seed Proteins’ Anti-Obesity Potential through Lipase Inhibition Using In Silico, In Vitro and In Vivo Models

Coronado-Cáceres

Rabadán-Chávez

Mojica

et al. 2020

Foods

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The aim of this study was to determine the pancreatic lipase (PL) inhibitory effect of cocoa protein (CP) hydrolysates (CPH) using in silico and in vitro approaches, and an in vivo high-fat diet (HF) obese rat model. The results showed better theoretical affinity on PL for cocoa peptides EEQR, GGER, QTGVQ, and VSTDVNIE released from vicilin and albumins (−6.5, −6.3, −6.2, and −6.1 kcal/mol, respectively). Absorption, distribution, metabolism, and excretion (ADMET) prediction showed the human intestinal absorption (HIA) capacity of orlistat and eight cocoa peptides, demonstrating that they presented a low probability of toxicity with values lower than 0.6, while the orlistat has a high probability of hepatotoxicity with a mean value of 0.9. CPH (degree of hydrolysis of 55%) inhibited PL with an IC50 (concentration needed to inhibit 50% of enzyme activity) value of 1.38 mg/mL. The intragastric administration of 150 mg CP/kg/day to rats increased total lipids and triglycerides excretion in feces, ranging from 11% to 15% compared to the HF-diet. The HF + CP-diet also significantly decreased (p < 0.05) the apparent rate of fat absorption compared with the HF group. These results suggest that CP has anti-obesity potential by inhibiting PL, thus helping to prevent the development of non-communicable diseases.

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Section: Discussionmentioning

confidence: 99%

Cocoa (Theobroma cacao L.) Seed Proteins’ Anti-Obesity Potential through Lipase Inhibition Using In Silico, In Vitro and In Vivo Models

Coronado-Cáceres

Rabadán-Chávez

Mojica

et al. 2020

Foods

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“…Marine peptides help aid weight maintenance by reducing the post-prandial blood sugar levels (95, 96). Recently, Fan et al (97) reported the anti-obesity properties of Spirulina platensis -derived peptides which has attracted the focus once again to marine originated peptides. Chitosan, a polysaccharide is already known to modulate the level of serum leptin, C- reactive protein and inhibits the adipocyte differentiation in obese rats (98, 99).…”

Section: Marine Peptidesmentioning

confidence: 99%

Peptides and Peptidomimetics as Potential Antiobesity Agents: Overview of Current Status

Kumar

2019

Front. Nutr.

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There is a high occurrence of obesity worldwide without many new medications being approved for its treatment. Therefore, there is an urgent need to introduce new approaches for treating obesity. Bioactive peptides have been used to treat metabolic disorders- such as type-2 diabetes and obesity; while also possessing anti-oxidant, anti-inflammatory, anti-microbial, and anti-viral properties. However, the development of these peptides has taken backstage due to their size, reduced stability, poor delivery and bioavailability, fast rate of degradation etc. But with the emergence of newer techniques for multifunctional peptides, mimetics, peptide analogs, and aptamers, there is a sudden revival in this therapeutic field. An increased attention is required for development of the natural peptides from food and marine sources which can mimic the function of mediators involved in weight management to avoid obesity. Herein, the search for the structures of anti-obesity peptides was carried out in order to establish their potential for drug development in future. An extensive search for the current status of endogenous, food and marine peptides, with reference to novel and interesting experimental approaches based on peptidomimetics for controlling obesity, was performed. Apolipoprotein A-I (apoA-I), melanocortin-4 receptor (MC4R)-specific agonist, GLP-1 dual and triple agonists, neuropeptides and prolactin-releasing peptide mimetics were specifically examined for their anti-obesity role. Novel peptides, mimetics, and synthesis interventions are transpiring and might offer safer alternatives for otherwise scarcely available safe antiobesity drug. A deeper understanding of peptides and their chemistry through the use of peptide engineering can be useful to overcome the disadvantages and select best mimetics and analogs for treatment in future.

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“…Among them, food-derived peptides attract extensive attention because they have physiological functions beyond their high nutritional values and can act as drugs and functional products [11][12][13][14]. Fan et al (2018) purified and identified four peptides including NPVWKRK, NALKCCHSCPA, CANPHELPNK, and LNNPSVCDCDCMMKAAR from protein hydrolysate of Spirulina platensis. Among them, NPVWKRK and CANPHELPNK could significantly decrease the accumulation of TG dropping to 23.7% and 19.5% at a concentration of 600 µg/mL, respectively (p < 0.05) [15].…”

Section: Introductionmentioning

confidence: 99%

“…Fan et al (2018) purified and identified four peptides including NPVWKRK, NALKCCHSCPA, CANPHELPNK, and LNNPSVCDCDCMMKAAR from protein hydrolysate of Spirulina platensis. Among them, NPVWKRK and CANPHELPNK could significantly decrease the accumulation of TG dropping to 23.7% and 19.5% at a concentration of 600 µg/mL, respectively (p < 0.05) [15]. LPYP, IAVPGEVA, and IAVPTGVA from digesting glycinin could improve low-density lipoprotein (LDL) uptake through activating the LDLR-sterol regulatory element-binding protein (SREBP) pathway and act as a competitive inhibitor of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR) to inhibit the biosynthesis of cholesterol [7,16].…”

Section: Introductionmentioning

confidence: 99%

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Wang

Pan

et al. 2020

Applied Sciences

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In this work, the hypolipidemic activities of two pentapeptides (VIAPW and IRWWW) from miiuy croaker (Miichthys miiuy) muscle on oleic acid (OA)-induced lipid accumulation in HepG2 cells were investigated. VIAPW and IRWWW could significantly inhibit lipid accumulation induced by OA and decreased intracellular levels of intracellular triglyceride (TG) and total cholesterol (TC) in a dose-effect dependence manner. At the concentration of 100 μm, the TG levels of VIAPW (0.201 ± 0.006 mm) and IRWWW (0.186 ± 0.005 mm) were very (p < 0.01) and extremely (p < 0.001) significantly lower than those (0.247 ± 0.004 mm) of the OA model group; the levels of TC of VIAPW (45.88 ± 0.74 μg/mg protein) and IRWWW (41.02 ± 0.14 μg/mg protein) were very (p < 0.01) and extremely (p < 0.001) significantly lower than that (53.45 ± 0.10μg/mg protein) of the OA model group (p < 0.01). The hypolipidemic mechanisms of VIAPW and IRWWW were to down-regulate the expression levels of genes of SREBP-1c, SREBP-2, FAS, ACC, and HMGR in lipid synthesis and to up-regulate the expression levels of genes of PPARα, ACOX-1, and CPT-1 in lipid oxidation. These results suggested that VIAPW and IRWWW could play their hypolipidemic activities in HepG2 cells through regulation of AMPK pathway and act as hypolipidemic nutrient ingredients applied in public healthy and functional foods.

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Purification and identification of anti-obesity peptides derived from Spirulina platensis

Cited by 69 publications

References 19 publications

Cocoa (Theobroma cacao L.) Seed Proteins’ Anti-Obesity Potential through Lipase Inhibition Using In Silico, In Vitro and In Vivo Models

Cocoa (Theobroma cacao L.) Seed Proteins’ Anti-Obesity Potential through Lipase Inhibition Using In Silico, In Vitro and In Vivo Models

Peptides and Peptidomimetics as Potential Antiobesity Agents: Overview of Current Status

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

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