Purification and comparison of intracellular proteinase A in Candida spp. isolates from Malaysian and Iranian patients and infected mice

Saroukolaei, Shahla Amri; Pei, Chong Pei; Shokri, Hojjatollah; Asadi, Farzad

doi:10.1016/j.mycmed.2012.01.002

Cited by 2 publications

(3 citation statements)

References 25 publications

(24 reference statements)

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“…Intracellular proteinase A is one of the most important enzymes in C. albicans that is expressed by the APR1 gene. This enzyme has an important role in cell survival under stress conditions in C. albicans , and the amount of this enzyme increases in the first few hours of nitrogen starvation when compared to mycelium growth ( 24 , 25 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of APR1 Gene Expression in Candida albicans Strains Isolated From Patients With Multiple Sclerosis

Saroukolaei

Ghabaee

Shokri

et al. 2016

Jundishapur J Microbiol

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BackgroundIntracellular aspartic proteinase A enzyme is expressed by the APR1 gene and is one of the important factors in the development of systemic candidiasis caused by Candida albicans.ObjectivesThe aim of this study was to evaluate the expression of the APR1 gene in C. albicans isolates obtained from patients with multiple sclerosis (MS) and from controls.Patients and MethodsThe samples were obtained from 135 MS patients with candidiasis and 100 matched controls of healthy individuals during 2010 - 2011. The clinical and control isolates of C. albicans obtained from individuals were cultured onto sabouraud dextrose agar (SDA). The evaluation of APR1 gene expression was performed using the reverse transcriptase-polymerase chain reaction (RT-PCR) method.ResultsThere was a statistically significant difference in APR1 gene expression of C. albicans strains between MS patients (mean ± SD: 0.5208 ± 0.11518) and the control group (mean ± SD: 0.7603 ± 0.11405) (P = 0.000). Significant correlations were found between the APR1 gene expression of C. albicans strains from MS patients with regard to age and the expanded disability status scale (EDSS) (P = 0.000). The mean values of EDSS were 1.6074 ± 0.1081 after antifungal treatment and 2.2519 ± 0.1323 before antifungal treatment (P = 0.000). No significant correlation was observed between the APR1 gene expression with regard to sex and MS subtypes.ConclusionsThe results suggested that APR1 gene expression in C. albicans strains isolated from MS patients may be an important factor for invasive C. albicans strains in the progression of MS disease.

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Section: Discussionmentioning

confidence: 99%

Evaluation of APR1 Gene Expression in Candida albicans Strains Isolated From Patients With Multiple Sclerosis

Saroukolaei

Ghabaee

Shokri

et al. 2016

Jundishapur J Microbiol

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“…In order to determine proteinase A in the extract collected from affinity chromatography column, mini‐protein 3 and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE) was done with electrophoresis proteins migrated through a 7.5% (w/v) polyacrylamide gel according to the size and molecular weight . To confirm the existence of proteinase A, we used the result of mini‐protein 3 SDS‐PAGE in immunoblotting.…”

Section: Methodsmentioning

confidence: 99%

“…This method was performed using two primary and secondary antibodies. We used two kinds of antibodies; primary polyclonal antibody against proteinase A (rose in rabbit‐ Razi institute) and secondary goat anti‐rabbit antibody conjugated to alkaline phosphatase (Razi institute) …”

Section: Methodsmentioning

confidence: 99%

The role of Candida albicans in the severity of multiple sclerosis

Saroukolaei

Ghabaee

Shokri

et al. 2016

Mycoses

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The purpose of this study was to compare the specific activity of proteinase A in Candida albicans (C. albicans) between multiple sclerosis (MS) patients and controls. A total of 135 and 100 C. albicans strains were isolated from superficial surfaces of MS patients and healthy controls. Analytical models (regression and neural network) were applied to predict the severity of MS considering specific enzyme activity (SEA) and other factors which affect the expanded disability status scale (EDSS). The SEA of C. albicans in MS patients (3466.95 ± 277.25 μmol min mg ) was significantly more than that of healthy controls (1108.98 ± 294.51 μmol min mg ) that was confirmed by regression model (P < 0.001). The SEA had a positive correlation with the severity of MS (P < 0.001, r = 0.65). Analytical models showed that SEA played the most important role (among all included factors that affect on EDSS) in the severity of MS. The SEA of C. albicans in MS patients was significantly more than the healthy controls. The results suggest that the level of SEA of proteinase A and probably the capacity of C. albicans isolates to invade the host tissue is associated with the severity of MS.

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Purification and comparison of intracellular proteinase A in Candida spp. isolates from Malaysian and Iranian patients and infected mice

Cited by 2 publications

References 25 publications

Evaluation of APR1 Gene Expression in Candida albicans Strains Isolated From Patients With Multiple Sclerosis

Evaluation of APR1 Gene Expression in Candida albicans Strains Isolated From Patients With Multiple Sclerosis

The role of Candida albicans in the severity of multiple sclerosis

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