Purification and Characterization of Two Novel Antimicrobial Peptides Subpeptin JM4-A and Subpeptin JM4-B Produced by Bacillus subtilis JM4

Abstract

An antimicrobial peptides-producing strain was isolated from soil and identified as Bacillus subtilis JM4 according to biochemical tests and 16S rDNA sequence analysis. The corresponding antimicrobial peptides were purified to homogeneity by ammonium sulfate precipitation, sequential SP-Sepharose Fast Flow, Sephadex G-25 and C18 reverse-phase chromatography, and in the final purification step, two active fractions were harvested, designated as Subpeptin JM4-A and Subpeptin JM4-B. The molecular weights, determined by mass spectrometry, were 1422.71 Da for Subpeptin JM4-A and 1422.65 Da for Subpeptin JM4-B, respectively. Amino acid sequencing showed that they differed from each other only at the seventh amino acid except for three unidentified residues, and the two peptides had no significant sequence homology to the known peptides in the database, indicating that they are two novel antimicrobial peptides. In addition, characteristic measurements indicated that both peptides had a relatively broad inhibitory spectrum and remained active over a wide pH and temperature range.

Purification and characterization of macrolactins and amicoumacins from Bacillus licheniformis BFP011: a new source of food antimicrobial substances

“…Antibiotics derived from Bacillus sp. were shown to inhibit the growth of Gram-positive and Gram-negative human pathogenic bacteria and phytopathogens (Lee et al, 2012;Wu et al, 2005). Apart from peptides, polyketides are the other dominant family of secondary metabolites having antimicrobial, immunosuppressive, antitumor or other physiologically relevant bioactivities.…”

Identification and Molecular Characterization of Bacteria Having Antimicrobial and Antibiofilm Activity

“…Though nisin is approved as food biopreservative, it has proved its potential for other biomedical applications as well [22]. Apart from LAB, antimicrobial substance from B. subtilis has also been reported against the broader range of bacteria inclusive of Salmonella, Bacillus cereus and Staphylococcus aureus [23]. B. subtilis can produce wide array of antimicrobial substances having varied biochemical and biological properties.…”

Antimycobacterial activity of a Brevibacillus Laterosporus strain isolated from a moroccan soil

“…Bacillus is a bacterial genus that is abundant in soil and contains several species that produce a large number of antibiotics with different chemical structures (29). These …”

“…A precipitation with 80% saturated ammonium sulfate was carried out overnight at 4°C under agitation (29). The To establish the protein nature of the antimycobacterial metabolite, the metabolite was also tested for its sensitivity to proteinase K (29).…”

“…The To establish the protein nature of the antimycobacterial metabolite, the metabolite was also tested for its sensitivity to proteinase K (29). Two controls were used: (1) the ammonium sulfate extract untreated by proteinase K and (2) proteinase K without the extract.…”