Purification and characterization of three alkaline proteases from the gut of the larva of army worm, Spodoptera litura

Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)

“…SKTI and CpTI (cowpea trypsin inhibitor) were shown to be active against tomato moth larvae (L. oleracea). Serine proteinases [24] acted on H. armigera, H. virenscens and L. cuprina, in which SKTI was the most effective among the inhibitors tested [54][55][56][57][58][59]. ApTI (Adenanthera pavonina trypsin inhibitor) and DMTI-II (Dimorphandra mollis trypsin inhibitor) inhibited 84% [20] and 80% [47] of the digestive trypsin-like enzymes of C. maculatus, a Colepteran (Bruchidae).…”

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

“…Trypsin was assayed with specific substrate, BApNA. This substrate is specific for the determination of trypsin activity (Ahmad et al 1980;Christeller et al 1992;Johnston et al 1995). The evidence for chymotrypsin-like activity in the alimentary tracts of Lepidoptera is less clear than that for trypsin-like activity.…”

Characterization of Proteolytic and Collagenolytic Enzymes from the Larvae of Lucilia cuprina, the Sheep Blowfly

“…The results of the inhibitory studies showed that PMSF, which is known to react specifically with functional serine residues (Ahmad et al 1980) and aprotinin, inhibited all of the neutral and alkaline proteases of L. cuprina, suggesting an essential role for serine in the catalytic function of these enzymes (Dixon et al 1956). PMSF had a lesser effect on the protease in the acidic region, though this may be due to the amount of enzyme in this region.…”