Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Ghodke, Amol Bharat; Chavan, Sonali; Sonawane, Balasaheb V.; Bharose, A. A.

doi:10.1080/17429145.2012.668952

Cited by 13 publications

(10 citation statements)

References 51 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some authors have attributed this effect to the presence of peptidase inhibitors, which can be expressed by plants as a defense mechanism (Gahloth et al 2011, Macedo et al 2011, Cruz et al 2013, Ghodke et al 2013 resulting in delayed development of insects.…”

Section: Insecticidal Activity Of Hexane Ethyl Acetate and Ethanol mentioning

confidence: 99%

Biological Activity of Piper aduncum extracts on Anticarsia gemmatalis (Hübner) (Lepidoptera: Erebidae) and Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Lucena

Bertholdo-Vargas

Silva

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Piper aduncum found naturally in the Amazon and southeastern Brazil, is known for its secondary metabolites that have activity on insects. Anticarsia gemmatalis and Spodoptera frugiperda are among the major insect pests associated with agricultural production. This research evaluated the biological activity of hexane, ethyl acetate, and ethanol extracts of P. aduncum leaves on mortality and duration of larval and pupal periods, as well as weight, width, and length of A. gemmatalis and S. frugiperda pupae. The mortality of A. gemmatalis larvae in trials with P. aduncum extracts were 93.3% (hexane) and 90% (ethyl acetate), estimating LC 50 of 6.35 and 5.79 mg/mL, respectively. Mortality in S. frugiperda submitted to the hexane extract ranged from 3.33% to 96.66% (LC 50 of 8.22 mg/mL). The ethanol extract induced low mortality (3.33% to 23.33%). The P. aduncum extracts did not affect the development of S. frugiperda pupae. In A. gemmatalis differences in weight and length occurred. The chemical characterization was by GC-MS, which revealed that the major constituent in the hexane extract of P. aduncum was apiol (90.7%). P. aduncum extracts are important and promising components to manage A. gemmatalis and S. frugiperda, which cause extensive production losses.

show abstract

Section: Insecticidal Activity Of Hexane Ethyl Acetate and Ethanol mentioning

confidence: 99%

Biological Activity of Piper aduncum extracts on Anticarsia gemmatalis (Hübner) (Lepidoptera: Erebidae) and Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Lucena

Bertholdo-Vargas

Silva

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…The number of localized responses that would be aided by the increased specificity afforded by obtaining quality RNA from tissue sections is vast (Santos et al 2012;Ghodke et al 2013;Gupta et al 2013;Ozawa et al 2013). For example, plant parasitic nematodes cause as much as 100-150 billion dollars in damage annually, worldwide (Sasser & Freckman 1987;Chitwood 2003).…”

Section: The Collection Of Semi-thin Sectionsmentioning

confidence: 99%

“…This problem would represent a major challenge for genomics studies involving plant-organism interactions because of the varied position of the pathogen, the limited amount of sample, and technical problems involved in isolating individual cells. However, the number of localized responses that would be aided by the increased specificity afforded by obtaining quality RNA from semi-thin sections is extensive (Santos et al 2012;Ghodke et al 2013;Gupta et al 2013;Ozawa et al 2013).…”

Section: Plastic Embedding and Single-cell Gene Studymentioning

confidence: 99%

Laser microdissection of semi-thin sections from plastic-embedded tissue for studying plant–organism developmental processes at single-cell resolution

Klink

Thibaudeau

2014

Journal of Plant Interactions

View full text Add to dashboard Cite

“…Of the four classes of proteolytic enzymes, serine proteases are among the best studied proteases from the insect mid gut (Hau andBenjakul 2006, Mohammadi et al, 2010). Larval mid gut contains a complex enzymatic system which helps in protein digestion and studies on the digestive proteases of lepidopteran insects have revealed that 95% of total digestive activity relies on serine proteases (Ghodke et al, 2013). Serine proteases, trypsin and chymotrypsin predominate digestive tract of Helicoverpa armigera.…”

Section: Introductionmentioning

confidence: 99%

Insecticidal Potential of Abelmoschus moschatus Trypsin Inhibitor (AMTI-II) Against Mid Gut Protease of Helicoverpa armigera

Kumar¹

2017

IJASR

View full text Add to dashboard Cite

show abstract

Isolation and in vitro identification of proteinase inhibitors from soybean seeds inhibiting helicoverpa gut proteases

Cited by 13 publications

References 51 publications

Biological Activity of Piper aduncum extracts on Anticarsia gemmatalis (Hübner) (Lepidoptera: Erebidae) and Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Biological Activity of Piper aduncum extracts on Anticarsia gemmatalis (Hübner) (Lepidoptera: Erebidae) and Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Laser microdissection of semi-thin sections from plastic-embedded tissue for studying plant–organism developmental processes at single-cell resolution

Insecticidal Potential of Abelmoschus moschatus Trypsin Inhibitor (AMTI-II) Against Mid Gut Protease of Helicoverpa armigera

Contact Info

Product

Resources

About