2013
DOI: 10.4172/1948-5948.1000109
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Purification and Characterization of Superoxide Dismutase Isolated From Sewage Isolated E. coli

Abstract: Superoxide dismutase (SOD), which plays a very important role in protecting organisms from oxygen toxicity, has therapeutic importance. It was purified from sewage isolated E. coli and characterized. Eukaryotic cells also produce SOD but culturing and maintenance of eukaryotic cells for production of SOD is costly as well as difficult. Using prokaryotic cells i.e. bacteria, production cost can be reduced. A rich bacterial source was identified. Bacterial membrane was ruptured in the presence of lysozyme and gl… Show more

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“…SOD was isolated from B. cereus bacteria, which thrive in an ideal environment at 370°C and pH 7, culminating in the highest extraction. Moreover, sonication induces alterations in the cellular membrane structure, resulting in the liberation of molecules and the diminishment of their sizes (Petkar et al 2013). The SOD principal objective in cells is to guard against and minimize oxidative damage induced by reactive oxygen radicals.…”
Section: Discussionmentioning
confidence: 99%
“…SOD was isolated from B. cereus bacteria, which thrive in an ideal environment at 370°C and pH 7, culminating in the highest extraction. Moreover, sonication induces alterations in the cellular membrane structure, resulting in the liberation of molecules and the diminishment of their sizes (Petkar et al 2013). The SOD principal objective in cells is to guard against and minimize oxidative damage induced by reactive oxygen radicals.…”
Section: Discussionmentioning
confidence: 99%