Purification and characterization of moschins, arginine–glutamate-rich proteins with translation-inhibiting activity from brown pumpkin (Cucurbita moschata) seeds

Ng, T.B.; Parkash, A.; Tso, Wung-Wai

doi:10.1016/s1046-5928(02)00500-4

Cited by 24 publications

(10 citation statements)

References 8 publications

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“…A peptide (MW: 8 kDa) from pumpkin seeds was proved to inhibit B. cinerea, F. oxysporum and M. arachidicola at a dose of 375 ug and to exerted an inhibitory effect on cell-free translation with an IC50 of 1.2 uM [47]. The purified α-moschin and β-moschin, two proteins with a molecular mass of 12 kDa from fresh brown pumpkin seeds, displayed translation-inhibiting activity with IC 50 of 17 uM and 300 nM, respectively [44]. A significant inhibitory effect of a purified protein (MW28000) against the fungal growth of Fusarium oxysporum was exerted in an agardisc plate at a concentration greater than 2 mM.…”

Section: Antidiabetic Activitymentioning

confidence: 97%

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A Review on Pharmacological Activities and Utilization Technologies of Pumpkin

Chen

Huan

2006

Plant Foods Hum Nutr

322

203

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Dietary plants and herbal preparations have been traditionally used as medicine in developing countries and obtained a resurgence of use in the United States and Europe. Research carried out in last few decades has validated several such claims of use of traditional medicine plants. Popularity of pumpkin in various systems of traditional medicine for several ailments (antidiabetic, antihypertensive, antitumor, immunomodulation, antibacterial, antihypercholesterolemia, intestinal antiparasitia, antiinflammation, antalgic) focused the investigators' attention on this plant. Considerable evidence from several epidemiological studies concerning bioactivities leads have stimulated a number of animal model, cell culture studies and clinical trials designed to test this pharmacological actions. In addition, it was found that technologies such as germination and fermentation could reduce antinutritional materials and affect the pharmacological activities of pumpkin. This review will focus on the main medicinal properties and technologies of pumpkin, and point out areas for future research to further elucidate mechanisms whereby this compound may reduce disease risk.

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Section: Antidiabetic Activitymentioning

confidence: 97%

“…Hypoglycemic activity of polysaccharide isolated from pumpkin containing 8.48% sugar was lower than that from pumpkin containing 4.29% sugar [43]. [44][45][46][47].…”

Section: Phytochemistry and Technologymentioning

confidence: 99%

A Review on Pharmacological Activities and Utilization Technologies of Pumpkin

Chen

Huan

2006

Plant Foods Hum Nutr

322

203

View full text Add to dashboard Cite

show abstract

“…A significant inhibitory effect of a purified protein (MW 28 kDa) against the fungal growth of Fusarium oxysporum was exerted in an agar disc plate at a concentration greater than 2 m m . This protein possessed a synergistic effect with nikkomycin, a chitin synthase inhibitor, for the growth inhibition of Candida albicans ( 48 ) . Three pumpkin seed basic proteins, MAP2 (MW 2·2 kDa), MAP4 (MW 4·6 kDa) and MAP11 (MW 11·7 kDa), have been shown to inhibit the growth of yeast cells, with MAP11 being the most effective inhibitor.…”

Section: Medicinal Bioactivities Of Pumpkinmentioning

confidence: 99%

Medicinal and biological potential of pumpkin: an updated review

et al. 2010

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The use of herbal remedies individually or in combination with standard medicines has been used in various medical treatises for the cure of different diseases. Pumpkin is one of the well-known edible plants and has substantial medicinal properties due to the presence of unique natural edible substances. It contains several phyto-constituents belonging to the categories of alkaloids, flavonoids, and palmitic, oleic and linoleic acids. Various important medicinal properties including anti-diabetic, antioxidant, anti-carcinogenic, anti-inflammatory and others have been well documented. The purpose of the present article is to discuss various medicinal and biological potentials of pumpkin that can impart further research developments with this plant for human health benefits.

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“…Ng et al. (2002) extracted protein from fresh seeds of ripe brown pumpkins using 0.1‐M Tris‐HCl buffer (pH 7.2), and arginine‐glutamate‐rich proteins were obtained. Different solvent used in protein extraction showed different efficiency for varies plant; in this experiment, 0.15‐M Tris‐HCl (pH 9.0, contain 0.15‐M NaCl, 2%PVPP) buffer was used as solvent for protein extraction showed a good result.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound‐microwave assisted extraction of proteins from Moringa oleifera leaves: Comparative optimization study and LC‐MS analysis of the protein concentrate

Cheng

Shu

Chen

et al. 2021

J. Food Process. Preserv.

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Moringa oleifera leaves contain significant amount of high quality protein which can be utilized as nutrition supplements and food functional ingredients. In this study, the extraction process of M. oleifera leaves protein was optimized by response surface methodology. Traditional solvent extraction conditions for maximum protein recovery were 0.15‐M Tris‐HCl concentration, 38‐min extraction time, and 128:1 (v/w) solvent/solid ratio at 34°C, and the protein yield was 68.99 mg/g. Furthermore, ultrasound‐microwave assisted extraction (UMAE) was adopted to product the leaves protein, and the results showed that the optimum extraction conditions were solvent/solid ratio 91:1 (v/w), extraction time 148 s, extraction temperature 41°C, microwave power 81 W, the yield of protein was 82.07 mg/g. Compared with the conventional solvent extraction method, UMAE exhibited significantly high extraction efficiency. Through LC‐MS technology identification and bioinformatics analysis, it is verified that the extracted proteins could provide energy and material basis for the functions of M. oleifera leaf. Practical applications Moringa oleifera leaves have a high protein content and reasonable composition of essential amino acids, which is a high‐quality plant protein with great development potential. In the present study, the response surface method was used to optimize the process of ultrasonic and microwave synergistic extraction of M. oleifera leaves protein, which is effective and significantly increased the protein yield. According to the results of LC‐MS identification and bioinformatics analysis, the proteins played a leading role in maintaining the normal tissue, structure, and function of M. oleifera leaves, participated in the growth and metabolism of M. oleifera leaves, and provided material and energy supply for the functional performance of M. oleifera leaves.

show abstract

Purification and characterization of moschins, arginine–glutamate-rich proteins with translation-inhibiting activity from brown pumpkin (Cucurbita moschata) seeds

Cited by 24 publications

References 8 publications

A Review on Pharmacological Activities and Utilization Technologies of Pumpkin

A Review on Pharmacological Activities and Utilization Technologies of Pumpkin

Medicinal and biological potential of pumpkin: an updated review

Ultrasound‐microwave assisted extraction of proteins from Moringa oleifera leaves: Comparative optimization study and LC‐MS analysis of the protein concentrate

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