A Review on Pharmacological Activities and Utilization Technologies of Pumpkin

Chen, Fu; Huan, Shi; Li, Quanhong

doi:10.1007/s11130-006-0016-6

Cited by 302 publications

(203 citation statements)

References 63 publications

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“…Cucurbita species are commonly used as vegetables in daily life as food, feed, for ornamental purposes. Medicinal properties have also been reported in this species (Caili et al 2006). Global production of Cucurbita species reached 21 million tons in 2009 (http://faostat.fao.org).…”

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confidence: 85%

Improving the transformation efficiency of Cucurbita species: factors and strategy for practical application

Nanasato

Okuzaki

Tabei

2013

Plant Biotechnology

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Cucurbita species are refractory to transformation. Hence, the only 2 reports published regarding the transformation of Cucurbita species until our successful transformation of C. moschata (cv. Heiankogiku) in 2011. The efficiency was 2.7±1.3% using wounded explants vortexed with whisker suspension. To improve transformation efficiency, transformation experiments were carried out with various ages of cotyledonary explants. The highest efficiency was obtained with 1-day-old (3.2±0.9%) and 2-day-old (3.3±0.8%) explants after germination. Histochemical analysis of GUS activity revealed that wounding allowed Agrobacterium access to the deeper layer of explants. These results suggested that cells having a high ability of shoot organogenesis exist not on the surface but in the deeper layers of explants. We applied vacuum infiltration to wounded explants to enhance Agrobacterium access to the deeper layers, which resulted in improvement of transformation efficiency by 3-fold (9.2±2.9%). To verify the efficacy of our transformation procedure for other Cucurbita species, we attempted to obtain transformants with 3 Cucurbita species: C. maxima (cv. Ebisu), C. pepo (cv. Black Tosca), and C. ficifolia (cv. Kurotanenankin). The average regeneration efficiencies were 54.2±7.2%, 62.5±19.1%, and 72.2±13.4% under our regeneration system. Although the efficiency for production of tansgenic plants was very low (ca. 0.2-0.3%), a transgenic line was obtained from C. maxima and C. pepo, respectively. We discuss recent advances that may help in the development of beneficial applications for the transformation of Cucurbita species.

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confidence: 85%

Improving the transformation efficiency of Cucurbita species: factors and strategy for practical application

Nanasato

Okuzaki

Tabei

2013

Plant Biotechnology

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“…Cv. This species, C. moschata has been considered to have many functions for human health as antimicrobial and to preserve kidney function (Caili et al 2006, El-Aziz & El-Kalek 2011. Because of the high content of carbohydrates and fibre, this vegetable plant has been performed as a food with an esteemed source of dietary fiber in human nutrition (Hussain et al 2010) may decrease the serum cholesterol level, the risk of coronary heart disease, and hypertension.…”

Section: Introductionmentioning

confidence: 99%

Interaction of a fly ash and root-knot nematode pathogens on Pumpkin (Cucurbita moschata Duch. ex Lam.)

Ahmad¹,

Khan²,

Ansari³

2017

Trop. Plant Res.

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Abstract:In the present investigation it was tried to find out the interactive effect of various level of fly ash (abiotic) and root-knot nematode, Meloidogyne incognita (biotic) on plant growth, yield and some biochemical contents in pumpkin (Cucurbita moschata). The experiments were conducted in pots with different level of fly ash and soil mixture in the green house at Department of Botany, Aligarh Muslim University, Aligarh, India. Seeds of pumpkin (F1 hybrid-Nutan) were grown in pots filled with different level of fly ash (5, 10, 20, 30, 40 and 50%). At four leaves stage, seedlings were inoculated with 2000 larvae (J2 stage) of M. incognita. Plants grown in soil (uninoculated and inoculated stes) serve as control for comparing with different level of fly ashamended soil. Plant growth and yield parameters were increased significantly in 10 to 30% fly ash amended soils. However, at higher level of fly ash (40% and 50%), plant growth and yield were reduced significantly. The suppression in plant growth and yield were maximum at 50% fly ash level. Similarly the chlorophyll, carotenoids, carbohydrate and proline contents were also increased at 10-30% levels, then increased in 40 and 50% of fly ash level compared to control (uninoculated set). All the parameters; growth, yield, chlorophyll, carotenoids, carbohydrate and proline content of plants were better for all the fly ash level as compared to nematode inoculated sets.

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“…In Greece pumpkin seeds are consumed either raw or roasted as component of the Mediterranean Diet. Several studies have dealt with the beneficial effects of the consumption of pumpkin flesh (Caili, Huan, & Quanhong, 2006), but limited studies exist on the pumpkin seeds. Although Cucurbita moschata is locally grown in the Greek island of Lemnos and is traditionally used in pumpkin pies the seeds are currently discarded after consumption of pumpkin flesh.…”

Section: Introductionmentioning

confidence: 99%

In vitro health beneficial activities of Pumpkin seeds from Cucurbita moschata cultivated in Lemnos

Sakka

Karantonis

2015

IJFS

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Pumpkin seeds are commonly consumed in Greece. Although Cucurbita moschata is locally grown in Lemnos and is traditionally used in pumpkin pies, the seeds are currently discarded after consumption of the fruit flesh. The aim of the present study was to investigate the nutritional functionality of pumpkin seeds from Cucurbita moschata grown in Lemnos. Cucurbita moschatas' seeds, raw or roasted, were appropriately extracted and the results are presented for raw versus (vs) roasted seed extracts. The phenolic content was expressed as µg gallic acid/g of seeds according to Folin-Ciocalteau assay (370.3 ± 19.1 vs 551.0 ± 22.0). Antioxidant capacity was expressed as equivalent amount for 50% scavenging in mg of seeds for DPPH (50.03 ± 5.91 vs 25.82 ± 6.77) and ABTS (17.85 ± 0.77 vs 12.77 ± 0.76) assays, and as µmol of trolox/g of seeds for FRAP (1.19 ± 0.05 vs 2.50 ± 0.23) and CUPRAC (2.13 ±0.11 vs 3.25 ± 0.06) assays. Anti-inflammatory/antithrombotic and anti-diabetic activities were expressed as mg of seeds for 50% inhibition of platelet activating factor (0.62 vs 0.15) and as µg of seeds for 25% inhibition of alpha-glycosidase (40.0 vs 61.0) activities respectively. Moreover, anti-atherogenic activity was expressed as the % increase in lag time of human plasma oxidation (62.7 versus 163.2) Raw and roasted pumpkin seed extracts exert anti-oxidant, anti-thrombotic/anti-inflammatory, antiatherogenic and antidiabetic activities. Cucurbita moschata seeds may represent a novel opportunity for development of functional foods, with a local interest in Lemnos that would contribute also to the regional public health improvement.

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A Review on Pharmacological Activities and Utilization Technologies of Pumpkin

Cited by 302 publications

References 63 publications

Improving the transformation efficiency of Cucurbita species: factors and strategy for practical application

Improving the transformation efficiency of Cucurbita species: factors and strategy for practical application

Interaction of a fly ash and root-knot nematode pathogens on Pumpkin (Cucurbita moschata Duch. ex Lam.)

In vitro health beneficial activities of Pumpkin seeds from Cucurbita moschata cultivated in Lemnos

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