Purification and Characterization of Lacticin NK34 Produced by Lactococcus lactis NK34 against Bovine Mastitis

이나경,; 박여랑,; 김현욱,; 박용호,; 임성렬,; 김종만,; 김재명,; 남향미,; 정석찬,; 백현동,

doi:10.5851/kosfa.2008.28.4.457

Cited by 4 publications

(8 citation statements)

References 17 publications

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“…3 dialysis tube (molecular weight cutoff of 3,500; Spectrum Medical Industries, Los Angeles, CA). The collected samples were stored at −70°C after dialysis (Lee et al, 2008). Because lacticin NK34 was already proven to be a bacteriocin through our previous study, a partially purified form of lacticin NK34 was used in the present study.…”

Section: Preparation Of Lacticin Nk34mentioning

confidence: 99%

“…Partially purified lacticin NK34 peptide was tested against S. simulans isolated from subclinical bovine mastitis to ensure purification (Lee et al, 2008). The peptide was subsequently tested against additional bacterial strains associated with mastitis (n = 40).…”

Section: In Vitro Antimicrobial Activity Of Lacticin Nk34 Against Bovine Mastitis-causing Bacteriamentioning

confidence: 99%

“…We evaluated the antimicrobial effects of the Lc. lactis product lacticin NK34 (a nisin-like bacteriocin), isolated from jeotgal (Korean salted and fermented fish food), in our previous study (Lee et al, 2008). Lacticin NK34 supernatants demonstrated bactericidal effects against several bacterial strains associated with bovine mastitis.…”

Section: Introductionmentioning

confidence: 96%

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In vitro antimicrobial effect and in vivo preventive and therapeutic effects of partially purified lantibiotic lacticin NK34 against infection by Staphylococcus species isolated from bovine mastitis

Kim

Shin

Koo

et al. 2010

Journal of Dairy Science

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Lantibiotics are small (<5 kDa), polycyclic peptides produced by gram-positive bacteria; they are also known as gram-positive bacteriocins. The high antimicrobial activity of lacticins and the continuing appearance of antibiotic-resistant bacteria in recent years have resulted in a renewed interest in lantibiotics. A partially purified form of lacticin NK34 (a Lactococcus lactis product isolated from the Korean fermented fish jeotgal) was tested to determine its antimicrobial effects against Staphylococcus aureus (n=20) and coagulase-negative Staphylococcus (CNS, n=20) strains isolated from the raw milk of cows with subclinical bovine mastitis in the present study. The spot-on-lawn assay was used to identify the 2 strains from each group with the greatest lacticin NK34 susceptibility, and the minimal lethal dose (MLD) was measured in ICR (imprinting control region) mice. The preventive and therapeutic effects of lacticin NK34 on the mouse infection model were determined for the first time. Lacticin NK34 demonstrated antimicrobial effects in 14 of 20 (70%) S. aureus indicator strains and in 18 of 20 (90%) CNS strains. Staphylococcus aureus 69 and S. simulans 55 demonstrated the greatest susceptibility to lacticin NK34 in the spot-on-lawn assay. The S. aureus 69 MLD was measured at 1.53 x 10(9) cfu/mouse, whereas the S. simulans 55 MLD was 3.59 x 10(9) cfu/mouse. Mice infected experimentally with S. aureus 69 MLD or S. simulans 55 MLD were treated with lacticin NK34. Treated mice demonstrated an 80% survival rate (48 of 60 mice) compared with a survival rate of 7.5% (3 of 40 mice) in control mice treated with distilled water. These data suggest that lacticin NK34 might be useful in the control of bovine mastitis and systemic bacterial infection.

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Section: Preparation Of Lacticin Nk34mentioning

confidence: 99%

Section: In Vitro Antimicrobial Activity Of Lacticin Nk34 Against Bovine Mastitis-causing Bacteriamentioning

confidence: 99%

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In vitro antimicrobial effect and in vivo preventive and therapeutic effects of partially purified lantibiotic lacticin NK34 against infection by Staphylococcus species isolated from bovine mastitis

Kim

Shin

Koo

et al. 2010

Journal of Dairy Science

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“…Bacterial strains and culture media P. pentosaceus SA131 was isolated from jeotgal, cul- Antimicrobial activity assay Pediocin SA131 activity was determined by the spoton-lawn method (Lee et al, 2008). Soft MRS agar seeded (1%, v/v) with the indicator microorganisms (ca.…”

Section: Methodsmentioning

confidence: 99%

“…The staphylococcal bacteriocins showed antibacterial activity against Staphylococcus aureus and Streptococcus agalactiae involved in bovine mastitis (Coelho et al, 2007). Also, lacticin NK34 was indentified as a bacteriocin having inhibitory activity against mastitis (Lee et al, 2008).…”

Section: Articlementioning

confidence: 99%

Medium Optimization for Pediocin SA131 Production by Pediococcus pentosaceus SA131 against Bovine Mastitis Using Response Surface Methodology

Park¹,

Lee²,

Park³

et al. 2010

Korean Journal for Food Science of Animal Resources

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Pediococcus pentosaceus SA131 was isolated from jeotgal, is the bacteriocin producer against bovine mastitis pathogens, Streptococcus uberis E290, Enterococcus gallinarum E362, and Staphylococcus epidermidis ATCC 12228. The medium composition for pediocin SA131 production by P. pentosaceus SA131 was optimized using response surface methodology. Component of medium was studied as carbon source (glucose, fructose, lactose, glycerol, sucrose, maltose, and mannitol), nitrogen source (beef extract, yeast extract, peptone, malt extract, and tryptone), mineral and surfactant (MgSO 4 , KH 2 PO 4 , (NH 4 ) 2 SO 4 , MnSO 4 , NaCl, sodium acetate, and Tween 80). Through one factor-at-a-time experiment, glucose, fructose, yeast extract, malt extract, NaCl, MgSO 4 , and Tween 80 were determined as the good ingredient. The effects of major factors for pediocin SA131 production were investigated by two-level fractional factorial designs (FFD). By a 2 4 FFD, fructose, yeast extract, and MnSO 4 were found to be the important factors for the bacteriocin production. Subsequently, a 2 3 central composite design (CCD) was adopted to derive a statistical model for optimizing the composition of the fermentation medium. The estimated optimum composition for the production of pediocin SA131 by P. pentosaceus SA131 was as follows; 0.13% fructose, 1% glucose, 1.8% yeast extract, 2.58% MnSO 4 , 0.2% NaCl, and 0.2% Tween 80. The pediocin production under optimized medium was increased to 1,000 AU/mL, compared to the 400 AU/mL in MRS medium.

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Evaluating the Translational Potential of Bacteriocins as an Alternative Treatment for Staphylococcus aureus Infections in Animals and Humans

Heinzinger,

Pugh,

Wagner

et al. 2023

Antibiotics

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Antibiotic resistance remains a global threat to human and animal health. Staphylococcus aureus is an opportunistic pathogen that causes minor to life-threatening infections. The widespread use of antibiotics in the clinical, veterinary, and agricultural setting combined with the increasing prevalence of antibiotic-resistant S. aureus strains makes it abundantly clear that alternatives to antibiotics are urgently needed. Bacteriocins represent one potential alternative therapeutic. They are antimicrobial peptides that are produced by bacteria that are generally nontoxic and have a relatively narrow target spectrum, and they leave many commensals and most mammalian cells unperturbed. Multiple studies involving bacteriocins (e.g., nisin, epidermicin, mersacidin, and lysostaphin) have demonstrated their efficacy at eliminating or treating a wide variety of S. aureus infections in animal models. This review provides a comprehensive and updated evaluation of animal studies involving bacteriocins and highlights their translational potential. The strengths and limitations associated with bacteriocin treatments compared with traditional antibiotic therapies are evaluated, and the challenges that are involved with implementing novel therapeutics are discussed.

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Purification and Characterization of Lacticin NK34 Produced by Lactococcus lactis NK34 against Bovine Mastitis

Cited by 4 publications

References 17 publications

In vitro antimicrobial effect and in vivo preventive and therapeutic effects of partially purified lantibiotic lacticin NK34 against infection by Staphylococcus species isolated from bovine mastitis

In vitro antimicrobial effect and in vivo preventive and therapeutic effects of partially purified lantibiotic lacticin NK34 against infection by Staphylococcus species isolated from bovine mastitis

Medium Optimization for Pediocin SA131 Production by Pediococcus pentosaceus SA131 against Bovine Mastitis Using Response Surface Methodology

Evaluating the Translational Potential of Bacteriocins as an Alternative Treatment for Staphylococcus aureus Infections in Animals and Humans

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