Medium Optimization for Pediocin SA131 Production by Pediococcus pentosaceus SA131 against Bovine Mastitis Using Response Surface Methodology

Abstract

Pediococcus pentosaceus SA131 was isolated from jeotgal, is the bacteriocin producer against bovine mastitis pathogens, Streptococcus uberis E290, Enterococcus gallinarum E362, and Staphylococcus epidermidis ATCC 12228. The medium composition for pediocin SA131 production by P. pentosaceus SA131 was optimized using response surface methodology. Component of medium was studied as carbon source (glucose, fructose, lactose, glycerol, sucrose, maltose, and mannitol), nitrogen source (beef extract, yeast extract, peptone, malt extract, and tryptone), mineral and surfactant (MgSO 4 , KH 2 PO 4 , (NH 4 ) 2 SO 4 , MnSO 4 , NaCl, sodium acetate, and Tween 80). Through one factor-at-a-time experiment, glucose, fructose, yeast extract, malt extract, NaCl, MgSO 4 , and Tween 80 were determined as the good ingredient. The effects of major factors for pediocin SA131 production were investigated by two-level fractional factorial designs (FFD). By a 2 4 FFD, fructose, yeast extract, and MnSO 4 were found to be the important factors for the bacteriocin production. Subsequently, a 2 3 central composite design (CCD) was adopted to derive a statistical model for optimizing the composition of the fermentation medium. The estimated optimum composition for the production of pediocin SA131 by P. pentosaceus SA131 was as follows; 0.13% fructose, 1% glucose, 1.8% yeast extract, 2.58% MnSO 4 , 0.2% NaCl, and 0.2% Tween 80. The pediocin production under optimized medium was increased to 1,000 AU/mL, compared to the 400 AU/mL in MRS medium.

Beneficial features of pediococcus: from starter cultures and inhibitory activities to probiotic benefits

“…By definition, bovine mastitis is an inflammatory disease of the mammary gland caused by the growth of different microorganisms (Turovskiy et al 2009). It was proposed that pediocin produced by P. pentocaceus SA131 (isolated from jeotgal) can be used as an agent for the treatment of bovine mastitis pathogens with effective inhibition of related pathogens, including Streptococcus uberis E290, Enterococcus gallinarum E362, and Staphylococcus epidermis ATCC 12228 (Park et al 2017).…”

Probiotic Properties of Pediococcus pentosaceus SH-10 Isolated from the Hard Clam Meretrix meretrix Shikhae

“…API 50CHL kit Pediococcus pentosaceus Pediococcus pentosaceus . P. pentosaceus 4 peptide pediocin (Facklam and Elliott, 1995;Osmanagaoglu et al, 2000;Park et al, 2010). (Matsumura et al, 1999;Mukai et al, 2004).…”