Purification and characterization of immunomodulatory peptides from enzymatic hydrolysates of duck egg ovalbumin

He, Ping; Wang, Qian; Qu, Zhan; Pan, Leiman; Xin, Xuan; Wu, Hui; Zhang, Mengmeng

doi:10.1039/d0fo02674c

Cited by 32 publications

(21 citation statements)

References 33 publications

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“…Macrophages are essential for the function of immune system because they may destroy infections both directly through phagocytosis and indirectly through the release of cytotoxic chemicals such as NO or cytokines such as TNF-α and IL-6 [ 35 ]. The effects of PEF-treated and untreated peptides on levels of TNF-α and IL-6 in RAW 264.7 cells were analyzed and are presented in Figure 2 D,E.…”

Section: Resultsmentioning

confidence: 99%

C-Terminal Modification on the Immunomodulatory Activity, Antioxidant Activity, and Structure–Activity Relationship of Pulsed Electric Field (PEF)-Treated Pine Nut Peptide

Zhang

Liu

Bao

et al. 2022

Foods

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In this study, a novel peptide VNAVL was synthesized by removing the C-terminal histidine on the basis of a bioactive peptide VNAVLH obtained from pine nut (Pinus koraiensis Sieb. et Zucc) protein. The effects of removing histidine on antioxidant activity, immunomodulatory activity, and secondary structure of the PEF-treated peptide were discussed. Compared with VNAVLH, VNAVL only exhibited lower antioxidant activity, but no immunomodulatory activity to release TNF-α, IL-6, and NO by activating RAW 264.7 cells. In addition, both antioxidant and immune activities of VNAVLH were significantly more sensitive to treatment with 40 kV/cm than other field intensities, whereas VNAVL was not sensitive to field strength changes. CD spectra and DSSP analysis verified that both peptides consisted of a β structure and random coil, but the ability of VNAVL to transform the random coil via PEF treatment is weaker than that of VNAVLH. Therefore, PEF treatment might expose the key active site located on the C-terminal histidine by altering the secondary structure of the peptide.

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Section: Resultsmentioning

confidence: 99%

C-Terminal Modification on the Immunomodulatory Activity, Antioxidant Activity, and Structure–Activity Relationship of Pulsed Electric Field (PEF)-Treated Pine Nut Peptide

Zhang

Liu

Bao

et al. 2022

Foods

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“…TLR 4 is an important part of the TLRs family ( 42 , 43 ). Numerous studies found that TLR 4 recognizes peptides and activates the downstream regulatory signal pathways, such as NF-κB and MAPK, to exert immunomodulatory effects ( 44 , 45 ). Our study showed that the gray value of TLR 4 bands was obviously in control, while the gray value of TLR 4 bands in the model was decreased ( Figure 7A ).…”

Section: Resultsmentioning

confidence: 99%

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

et al. 2022

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This study aimed to investigate the immunomodulatory activation of low-molecular-weight peptides from monkfish (Lophius litulon) roe (named MRP) on cyclophosphamide (CTX)-induced immunosuppressed mice. Our results indicated that MRP (100 mg/kg/d BW) could significantly increase the body weight and immune organ index, and improve the morphological changes in the spleen and thymus of mice. These effects subsequently enhance the serum levels of interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, and immunoglobulin (Ig) A, IgM, and IgG. Furthermore, MRP could also improve CTX-induced oxidative stress, and activate the NF-κB and MAPK pathways in the spleen tissues. The findings reported herein indicate that MRP has a good immunomodulatory activation toward immunosuppressed mice, hence can potentially be developed as an immune adjuvant or functional food.

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“…Egg white protein peptide oral liquid is a functional drink made of salted duck egg white through a series of processes such as desalting, enzymatic hydrolysis, and blending. Egg white protein peptide oral liquid is not only easy to digest, but also has a variety of biological activities (He et al, 2021).…”

Section: Desalting Utilization Of Salted Duck Egg Whitementioning

confidence: 99%

“…With a molecular weight of 45 kDa and an isoelectric point between 4.5 and 4.8, ovalbumin is the main protein in duck egg white, accounting for 54% of the total protein of duck egg white (He et al, 2021). Ovalbumin is a phosphoglycoprotein composed of 385 amino acids (Abeyrathne et al, 2014).…”

Section: Nutritional Value Of Salted Duck Egg Whitementioning

confidence: 99%

Mooncake production waste: Nutritional value and comprehensive utilization of salted duck egg white

Yao

Adhikari

et al. 2022

Food Processing Preservation

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Salted duck egg yolk is often used as stuffing of food products such as mooncakes.Due to high salt content, salted duck egg white is rarely used in food production and is often discarded. However, Salted duck egg white is rich in protein, amino acids, minerals, and vitamins, which can be fully absorbed and utilized by the human body.This review presented the nutritional value of salted duck egg white, including proteins, amino acids, minerals, and vitamins. Then, the review further analyzed and summarized the application of salted duck egg white, including directly utilized without desalination, desalting utilization, enzymatic extraction of active peptides, lysozyme extraction, and application of salted duck egg white in biomaterials. Finally, the future development and utilization perspectives of salted duck egg white were proposed. This literature review may provide theoretical references for the further development and utilization of salted duck egg white.Novelty impact statement: Salted duck egg white is rarely used in food production and is often discarded. However, Salted duck egg white is rich in nutrition. The review of nutritional value and comprehensive utilization of salted duck egg white may provide theoretical references for the further development of salted duck egg white.

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Purification and characterization of immunomodulatory peptides from enzymatic hydrolysates of duck egg ovalbumin

Abstract: Duck egg white (DEW) is considered as an abandoned protein resource.

Cited by 32 publications

References 33 publications

C-Terminal Modification on the Immunomodulatory Activity, Antioxidant Activity, and Structure–Activity Relationship of Pulsed Electric Field (PEF)-Treated Pine Nut Peptide

C-Terminal Modification on the Immunomodulatory Activity, Antioxidant Activity, and Structure–Activity Relationship of Pulsed Electric Field (PEF)-Treated Pine Nut Peptide

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

Mooncake production waste: Nutritional value and comprehensive utilization of salted duck egg white

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