Purification and characterization of hyaluronidase from oral Peptostreptococcus species

Tam, Y.; Chan, E. C. S.

doi:10.1128/iai.47.2.508-513.1985

“…The inability of P. tnicros counts to reliably predict future disease aetivity is consistent with the low prognostic capabilities expected of any narrowly based diagnostic test for progessive periodontitis (42), However, inclusion of P. micros in diagnostic analysis eneotnpassing several tnicrobiological and host variables is likely to enhance identification of patients and sites at high risk of periodontitis disease-activity. Pathogenic properties of P. tnicros of possible importance in periodontal disease pathogenesis include marked proteolytie enzytne activity (15,30,50), elaboration of hyaluronidase (46), production of volatile sulfur compounds from L-cysteine (35), and inhibition of human gingival fibroblast and epithelial cell growth (47). P. micros is able to Range of antimicrobial concentrations required to inhibit all strains tested.…”

Section: Discussionmentioning

confidence: 99%

Peptostreptococcus micros in human periodontitis

Rams

¹

,

Feik

²

,

Listgarten

³

et al. 1992

Oral Microbiology and Immunology

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Peptostreptococcus micros is a recognized pathogen in medical infections, and its association with progressive periodontitis was examined in this study. P. micros was isolated from paper-point subgingival samples on anaerobic enriched blood agar plates and identified on the basis of cellular and colonial morphology and selected biochemical tests. In a cross-sectional study involving 907 people with advanced adult periodontitis, 127 with early-onset periodontitis, and 12 with localized juvenile periodontitis, P. micros in these patient groups occurred with a prevalence of 58-63%. In culture-positive patients, P. micros averaged 12-15% of total viable counts. P. micros demonstrated similar occurrence and proportional recovery in all age groups. In a longitudinal study of 91 adult periodontitis patients on maintenance therapy, P. micros demonstrated a significantly higher prevalence in disease-active than in disease-inactive patients (47% vs 14%). Mechanical subgingival debridement and 0.12% chlorhexidine pocket irrigation was unable to eradicate subgingival P. micros from 18 of 22 adult periodontitis patients. In vitro antimicrobial susceptibility testing showed P. micros to be sensitive to therapeutic levels of penicillin, clindamycin and metronidazole. Our findings indicate that P. micros is a potential pathogen in adult periodontitis. The methods for its eradication from subgingival sites remain to be determined.

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“…Streptomyces hyalurolyticus, Streptomyces coelicolor and Streptomyces griseus produce enzymes capable of degrading hyaluronate [16]. A hyaluronidase purified and characterized from a species of Peptostreptococcus also has activity against chondroitin sulfate [17].…”

Section: Organisms Producing Hyaluronidasementioning

confidence: 99%

Hyaluronidases of Gram-positive bacteria

Hynes

¹

,

Walton

²

2000

FEMS Microbiology Letters

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Bacterial hyaluronidases, enzymes capable of breaking down hyaluronate, are produced by a number of pathogenic Gram-positive bacteria that initiate infections at the skin or mucosal surfaces. Since reports of the hyaluronidases first appeared, there have been numerous suggestions as to the role of the enzyme in the disease process. Unlike some of the other more well studied virulence factors, much of the information on the role of hyaluronidase is speculative, with little or no data to substantiate proposed roles. Over the last 5 years, a number of these enzymes from Gram-positive organisms have been cloned, and the nucleotide sequence determined. Phylogenetic analysis, using the deduced amino acid sequences of the Gram-positive hyaluronidases, suggests a relatedness among some of the enzymes. Molecular advances may lead to a more thorough understanding of the role of hyaluronidases in bacterial physiology and pathogenesis.

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“…Streptomyces hyalurolyticus, Streptomyces coelicolor and Streptomyces griseus produce enzymes capable of degrading hyaluronate [16]. A hyaluronidase puri¢ed and characterized from a species of Peptostreptococcus also has activity against chondroitin sulfate [17].…”

Section: Organisms Producing Hyaluronidasementioning

confidence: 99%

Hyaluronidases of Gram-positive bacteria

Hynes

¹

2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

Bacterial hyaluronidases, enzymes capable of breaking down hyaluronate, are produced by a number of pathogenic Gram-positive bacteria that initiate infections at the skin or mucosal surfaces. Since reports of the hyaluronidases first appeared, there have been numerous suggestions as to the role of the enzyme in the disease process. Unlike some of the other more well studied virulence factors, much of the information on the role of hyaluronidase is speculative, with little or no data to substantiate proposed roles. Over the last 5 years, a number of these enzymes from Gram-positive organisms have been cloned, and the nucleotide sequence determined. Phylogenetic analysis, using the deduced amino acid sequences of the Gram-positive hyaluronidases, suggests a relatedness among some of the enzymes. Molecular advances may lead to a more thorough understanding of the role of hyaluronidases in bacterial physiology and pathogenesis. ß

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Purification and characterization of hyaluronidase from oral Peptostreptococcus species

Cited by 50 publications

References 29 publications

Peptostreptococcus micros in human periodontitis

Peptostreptococcus micros in human periodontitis

Hyaluronidases of Gram-positive bacteria

Hyaluronidases of Gram-positive bacteria

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