Purification and characterization of hyaluronic acid from chicken combs

Rosa, Cláudia Severo da; Tovar, Ana Freire; Mourāo, Paulo A.S.; Pereira, Ricardo; Barreto, Pedro Luiz Manique; Beirão, Luiz Henrique

doi:10.1590/s0103-84782012005000056

Cited by 20 publications

(11 citation statements)

References 22 publications

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“…These values are much lower than those reported by Nakano and Sim and Nakano et al ., who recovered about 40 µg uronic acid mg −1 dried comb from 52‐week‐old roosters. This confirmed that the age of the birds also plays an important role, as reported by Rosa et al ., since our sample combs were obtained from 5–6‐week‐old broiler chickens, the major group of broilers processed in Thailand.…”

Section: Resultsmentioning

confidence: 99%

“…Chicken comb, another by‐product of the poultry industry, is, on the other hand, a rich source of hyaluronic acid (HA), which is widely used in a variety of medical and cosmetic applications . HA is a polysaccharide consisting of two alternating units of N ‐acetyl glucosamine and d ‐glucuronic acid linked by β ‐(1 → 4) and β ‐(1 → 3) linkages .…”

Section: Introductionmentioning

confidence: 99%

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Proteolytic activity from chicken intestine and pancreas: extraction, partial characterization and application for hyaluronic acid separation from chicken comb

et al. 2013

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteolytic activity from chicken intestine and pancreas: extraction, partial characterization and application for hyaluronic acid separation from chicken comb

et al. 2013

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“…Polysaccharides: hyaluronic acid, alginate, and chitosan. Another scaffold derived from nature and frequently used in central nervous system research is hyaluronic acid (HA) which is derived from chicken combs or vitreous humor (Hou et al, 2005;Rosa et al, 2012). HA is a glycosaminoglycan (GAG) and a major component of the natural ECM.…”

Section: Natural Polymersmentioning

confidence: 99%

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Groot

Sliedregt

Benthem

et al. 2019

The Anatomical Record

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In recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two‐dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibility of a treatment. Therefore, research is focusing on the strengths of stem cells in the three‐dimensional (3D) structures mimicking organs, that is, organoids, or organ‐on‐chip, for modeling human biology and disease. As 3D technology advances, it is necessary to know which signals stem cells need to multiply and differentiate into complex structures. This holds especially true for the complex 3D structure of the inner ear. Recent work suggests that although other factors play a role, the extracellular matrix (ECM), including its topography, is crucial to mimic a stem cell niche in vitro and to drive stem cells toward the formation of the tissue of interest. Technological developments have led to the investigation of biomaterials that closely resemble the native ECM. In the fast forward moving research of organoids and organs‐on‐chip, the inner ear has hardly received attention. This review aims to provide an overview, by describing the general context in which cells, matrix and morphogens cooperate in order to build a tissue, to facilitate research in 3D inner ear technology. Anat Rec, 303:408–426, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

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“…Previously, HA was extracted from rooster comb. According to a study conducted by Rosa et al (2012), they concluded that rooster comb was composed of about 90% HA, making it suitable for extraction. However, use of animal-derived biochemical can be risky due to zoonosis and unpremeditated infection transfer.…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture

Lai¹,

Teo²

2019

MJM

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Aims: Hyaluronic acid (HA) is a high molecular weight polymer and a major component of mucoid capsule in bacteria and extracellular matrix (ECM) of vertebrate tissue. Due to its unique characteristics, HA is used extensively in medical and cosmetic field. However, because of the exotoxins production from animal tissues extraction and Streptococcus zooepidemicus, HA production by recombinant microorganisms has gained interest. The present study was aimed at cloning of hasA gene in Escherichia coli and optimization of the medium components for HA production. Methodology and results: A fragment of an approximate size of 1.5 kb that encodes the hyaluronan synthase (hasA) gene from S. zooepidemicus ATCC 39920 was amplified by PCR using hasA-specific primers. The hasA gene was ligated into the bacterial expression vector pLbADH and transformed into the expression host, Escherichia coli BL21 strain. Then, genetically engineered E. coli strain BL21 was used for the production of HA by fermentation using different glucose concentration (10-50 g/L) and different IPTG concentration (0.1, 0.5 and 1.0 mM) in shake flask culture. Amongst varying glucose concentrations, results showed that 50 g/L glucose with nutrient rich media containing nitrogen source was able to produce the highest HA concentration (0.115 ± 0.002 g/L). With addition of 1.0 mM IPTG, HA production reached a peak 0.532 ± 0.026 g/L which is around fivefold higher compared to without IPTG. Conclusion, significance and impact of study: The hasA gene was cloned from S. zooepidemicus and successfully expressed in recombinant E. coli BL21 cells. This low molecular weight HA is gaining more importance in medical and cosmetic application due to possess pronounced free radical scavenging and antioxidant activities.

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Purification and characterization of hyaluronic acid from chicken combs

Abstract: Hyaluronic acid (HA) is an important macromolecule in medical and

Cited by 20 publications

References 22 publications

Proteolytic activity from chicken intestine and pancreas: extraction, partial characterization and application for hyaluronic acid separation from chicken comb

Proteolytic activity from chicken intestine and pancreas: extraction, partial characterization and application for hyaluronic acid separation from chicken comb

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture

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