Purification and characterization of cyanogenic β-glucosidase from wild apricot (Prunus armeniaca L.)

Characterization of a rutin-hydrolyzing enzyme with β-glucosidase activity from tartary buckwheat (Fagopyrum tartaricum) seeds

“…The optimum temperature required for the activity of FtRHE was similar to β-glucosidases from Trichoderma harzianum (Florindo et al, 2018), Prunus armeniaca (Bhalla et al, 2017) and Brassica oleracea (Bešić et al, 2017), which was lower than that from Anoxybacillus sp. (Chan et al, 2016), Sechium edule (Mateos et al, 2015), Nasutitermes takasagoensis (Uchima et al, 2012)and Thermoanaerobacterium thermosaccharolyticum (Pei et al, 2012) (Table 3).…”

Effect of ultrasonic pretreatment on eliminating cyanogenic glycosides and hydrogen cyanide in cassava

“…The highest activity of endogenous β-glucosidase in soybean occurred at 45℃ ultrasound as described in the previous study [19] . Reports on cyanogenic β-glucosidase activity had revealed the optimal temperature ranged from 35 to 55℃ in apricot and black plum seed [34] , [35] , [36] . Generally, low-intension and short-time ultrasound could activate the enzyme, but high-intension and long-time ultrasound may cause enzyme inactivation [17] , [18] , [25] , [37] .…”

Superior Plants with Significant Amounts of Cyanide and Their Toxicological Implications

“…The final hydrolysis reaction is the decomposition of mandelonitrile to benzaldehyde and HCN by hydroxynitrile lyase (Tomescu et al, 2020); this last phase is rapid and occurs at pH 10. The hydrolysis of amygdalin is catalyzed by the enzyme emulsin, a β-glucosidase (Bhalla et al, 2017), and by the bacterial flora; its enzymatic decomposition occurs rapidly under alkaline conditions (COT, 2006;Bolarinwa et al, 2014) and accelerates in the presence of heat. The clinical dose of amygdalin should not exceed 680 mg/kg (Jaswal et al, 2018).…”

“…CGs are secondary metabolites stored in cellular vacuoles of plant tissues and are composed of an aglycone type α-hydroxynitrile and a sugar like D-glucose (Vetter, 2000;Barceloux, 2009;Abraham et al, 2016). When the tissues are broken by mechanical action (crushing, chewing), the CGs make contact with endogenous lyase enzymes such as β-glucosidases and α-hydroxynitrile that result in the production of HCN (Bolarinwa et al, 2016;Bhalla et al, 2017); The HCN released by these cyanogenic plants protects them from attack, microbial, fungal, insects and herbivorous animals (INTA, 2011b;Kassim et al, 2014).…”