Purification and characterization of canine intestinal motilin

Poitras, Pierre; Reeve, Joseph R.; Hunkapiller, Michael W.; Hood, Leroy; Walsh, John H.

doi:10.1016/0167-0115(83)90251-3

Cited by 63 publications

(11 citation statements)

References 12 publications

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“…29,30) It has been also reported that dog and porcine motilins have an equal potency in inducing premature phase III of the migrating motor complex after intravenous injection to conscious dogs. 31) Hence, these results suggest that endogenous motilin of dog and SK-896 have an equal potency in stimulating the contractile activities of the gastrointestinal tract. From a comparison of the plasma concentrations of SK-896 and endogenously induced dog motilin after intravenous administration of SK-896 to dogs with postlaparotomy ileus, it is conceivable that the contractile activities of the gastrointestinal tract may be induced by both SK-896 and endogenous dog motilin.…”

Section: Discussionmentioning

confidence: 72%

Effects of SK-896, a New Human Motilin Analogue ([Leu13]motilin-Hse), on Postoperative Ileus in Dogs after Laparotomy.

Furuta

Takeda

Nakayama

et al. 2002

Biological & Pharmaceutical Bulletin

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The effects of SK-896, a new human motilin analogue ([Leu 13 ]motilin-Hse), on digestive tract motility in postoperative ileus were evaluated in a dog model of ileus after laparotomy. SK-896 was intravenously administered at 0.17, 0.33 and 0.67 m mg/kg starting soon after operation and then at 6-h intervals, for a total of 9 times. SK-896 progressively, dose-dependently and significantly increased the duodenal motility from 1 h after operation. The recovery time of the gastrointestinal-interdigestive migrating complex (GI-IMC) activity, which is an indicator of normal gastrointestinal tract activity after laparotomy, was 56.5؎5.0 h in the control group. SK-896 significantly shortened this recovery time. On the other hand, the plasma SK-896 concentrations declined diexponentially after administration, and can be described by a linear pharmacokinetic model within the dose range used. In addition, the pharmacokinetics of SK-896 did not change significantly at any postoperative time. There was no correlation between the plasma SK-896 concentrations and the intensity of duodenal motility, because the activity in the duodenum decreased transiently 13 h after laparotomy and increased with time thereafter. The changes in the activity are considered to reflect the progressive changes in the state of ileus. In conclusion, SK-896 increased the duodenal motility significantly, shortening the recovery time of GI-IMC-like activity in dogs with post-laparotomy ileus. Therefore, it is expected from these results that SK-896 would be useful and effective for the treatment of gastroparalysis after abdominal surgery.

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Section: Discussionmentioning

confidence: 72%

Effects of SK-896, a New Human Motilin Analogue ([Leu13]motilin-Hse), on Postoperative Ileus in Dogs after Laparotomy.

Furuta

Takeda

Nakayama

et al. 2002

Biological & Pharmaceutical Bulletin

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“…There are five amino acid differences between these sequences and that of canine motilin [15] at residues 7, 8, 12, 13 and 14 ( fig.2).…”

Section: Comparison Of Motilin Sequencementioning

confidence: 99%

Sequence of an intestinal cDNA encoding human motilin precursor

Seino¹,

Tanaka²,

Takeda

et al. 1987

FEBS Letters

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A cDNA clone encoding the human motilin precursor was isolated from an intestinal library using synthetic oligonucleotide probes. The predicted amino acid sequence indicates that the motilin precursor consists of 115 amino acids and includes a 25-residue N-terminal signal peptide followed by the 22-amino-acid motilin sequence and a long, 68-residue C-terminal peptide. The amino acid sequence of human motilin predicted from the cDNA sequence is indentical to its porcine counterpart, which has been determined by protein sequencing. Proteolytic processing of promotilin to motilin occurs at the sequence, Lys-Lys, this being the first reported instance of processing occurring at a pair of Lys residues. In other precursors it occurs at Lys-Arg, Arg-Arg, Arg, or very rarely Lys.cDNA sequence; Motilin; (Human)

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“…As early as 1981, SMITH et al suggested the possibility of a structural differences of motilin molecules in several mammalian tissues. Such structural differences for the motilin molecule have been confirmed to occur between the pig and dog (POITRAS et al, 1983;REEVE et al, 1985). Recently the heterogeneity of motilin molecules in the gastrointestinal mucosa and pancreas has been demonstrated in the reptile by an immunohistochemical study using region-specific motilin antisera in two species of caimans (Alligatorinae) This work was supported by a Research Grant-in-Aid (No.…”

Section: Discussionmentioning

confidence: 99%