Purification and Characterization of Antioxidant Peptides Derived from Protein Hydrolysate of the Marine Bivalve Mollusk Tergillarca granosa

Yang, Xiurong; Qiu, Yu; Zhao, Yu-Qin; Chi, Chang-Feng; Wang, Bin

doi:10.3390/md17050251

Cited by 60 publications

(59 citation statements)

References 55 publications

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“…The structure-activity relationship of the functional molecule will provide the basis for predicting the activity of new compounds, but so far it is still difficult to illuminate the relationship between structural characteristics and antioxidant activity of peptides [5,46]. In general, molecular size, hydrophobicity, and amino acid composition and sequence are regarded as key factors for the activity of antioxidant peptides [47,48].…”

Section: Structure-activity Relationship Of Mmp-4 Mmp-7 and Mmp-12mentioning

confidence: 99%

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Wang

Zhao

et al. 2020

Marine Drugs

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In the work, defatted muscle proteins of monkfish (Lophius litulon) were separately hydrolyzed by pepsin, trypsin, and in vitro gastrointestinal (GI) digestion methods, and antioxidant peptides were isolated from proteins hydrolysate of monkfish muscle using ultrafiltration and chromatography processes. The antioxidant activities of isolated peptides were evaluated using radical scavenging and lipid peroxidation assays and H2O2-induced model of HepG2 cells. In which, the cell viability, reactive oxygen species (ROS) content, and antioxidant enzymes and malondialdehyde (MDA) levels were measured for evaluating the protective extent on HepG2 cells damaged by H2O2. The results indicated that the hydrolysate (MPTH) prepared using in vitro GI digestion method showed the highest degree of hydrolysis (27.24 ± 1.57%) and scavenging activity on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (44.54 ± 3.12%) and hydroxyl radical (41.32 ± 2.73%) at the concentration of 5 mg protein/mL among the three hydrolysates. Subsequently, thirteen antioxidant peptides (MMP-1 to MMP-13) were isolated from MPTH. According to their DPPH radical and hydroxyl radical scavenging activity, three peptides with the highest antioxidant activity were selected and identified as EDIVCW (MMP-4), MEPVW (MMP-7), and YWDAW (MMP-12) with molecular weights of 763.82, 660.75, and 739.75 Da, respectively. EDIVCW, MEPVW, and YWDAW showed high scavenging activities on DPPH radical (EC50 0.39, 0.62, and 0.51 mg/mL, respectively), hydroxyl radical (EC50 0.61, 0.38, and 0.32 mg/mL, respectively), and superoxide anion radical (EC50 0.76, 0.94, 0.48 mg/mL, respectively). EDIVCW and YWDAW showed equivalent inhibiting ability on lipid peroxidation with glutathione in the linoleic acid model system. Moreover, EDIVCW, MEPVW, and YWDAW had no cytotoxicity to HepG2 cells at the concentration of 100.0 µM and could concentration-dependently protect HepG2 cells from H2O2-induced oxidative damage through decreasing the levels of reactive oxygen species (ROS) and MDA and activating intracellular antioxidant enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). These present results indicated that the protein hydrolysate and isolated antioxidant peptides from monkfish muscle, especially YWDAW could serve as powerful antioxidants applied in the treatment of some liver diseases and healthcare products associated with oxidative stress.

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Section: Structure-activity Relationship Of Mmp-4 Mmp-7 and Mmp-12mentioning

confidence: 99%

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Wang

Zhao

et al. 2020

Marine Drugs

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“…Two peptide hydrolysate fractions from the Marine Bivalve Mollusc, Tergillarca granosa, have also been demonstrated to exhibit antioxidant activity [57]. The peptides with amino acid composition (and thus designated) MDLFTE and WPPD exhibited strong DPPH free radical scavenging (EC50 = 0.53 and 0.36 mg/mL, respectively), hydroxyl radical scavenging (EC50 = 0.47 and 0.38 mg/mL respectively), superoxide anion radical (EC50 = 0.75 and 0.46 mg/mL, respectively) and ABTS cation radical scavenging (EC50 = 0.96 and 0.54 mg/mL, respectively) activities, although these antioxidant activities were not sustained at basic pH conditions (pH > 9 for 2.5 h), high temperatures (>80ºC for 0.5 h) nor during simulated GI digestion [57].…”

Section: Peptides With Antioxidant Activitymentioning

confidence: 99%

Bioactive Peptides from Marine Molluscs – A Review

Eghianruwa¹,

Osoniyi

Maina³

et al. 2019

IJBcRR

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Marine organisms make up approximately half of the total global biodiversity, with the Mollusca containing the second largest number of species, including snails and bivalves. The marine environment is highly competitive, hostile and aggressive, which has led to the production of specific and potent bioactive compounds by the mollusca and their associated microorganisms, in a bid to protect themselves and ensure their survival. A diverse array of bioactive compounds can be isolated from the extracts of marine molluscs of which linear, cyclic, and conjugated peptides and depsipeptides form some of the most important bioactive compounds that have been well characterized and some of have already reached clinical trials or been approved for use as therapeutic agents and supplements. This review highlights some of the bioactive peptides that have been obtained from marine molluscs as well the challenges facing bioprospecting of valuable peptides from marine mollusc sources.

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“…In recent years, seafood-derived peptides with antihypertensive, antioxidant, and antitumor activities have been characterized from many marine products and their by-products [10,11,[20][21][22]. In contrast, little attention has been paid to peptides with hypolepidemic, anti-obesity, antiatherogenic and cardioprotective effects [2].…”

Section: Introductionmentioning

confidence: 99%

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

Wang

Pan

et al. 2020

Applied Sciences

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In this work, the hypolipidemic activities of two pentapeptides (VIAPW and IRWWW) from miiuy croaker (Miichthys miiuy) muscle on oleic acid (OA)-induced lipid accumulation in HepG2 cells were investigated. VIAPW and IRWWW could significantly inhibit lipid accumulation induced by OA and decreased intracellular levels of intracellular triglyceride (TG) and total cholesterol (TC) in a dose-effect dependence manner. At the concentration of 100 μm, the TG levels of VIAPW (0.201 ± 0.006 mm) and IRWWW (0.186 ± 0.005 mm) were very (p < 0.01) and extremely (p < 0.001) significantly lower than those (0.247 ± 0.004 mm) of the OA model group; the levels of TC of VIAPW (45.88 ± 0.74 μg/mg protein) and IRWWW (41.02 ± 0.14 μg/mg protein) were very (p < 0.01) and extremely (p < 0.001) significantly lower than that (53.45 ± 0.10μg/mg protein) of the OA model group (p < 0.01). The hypolipidemic mechanisms of VIAPW and IRWWW were to down-regulate the expression levels of genes of SREBP-1c, SREBP-2, FAS, ACC, and HMGR in lipid synthesis and to up-regulate the expression levels of genes of PPARα, ACOX-1, and CPT-1 in lipid oxidation. These results suggested that VIAPW and IRWWW could play their hypolipidemic activities in HepG2 cells through regulation of AMPK pathway and act as hypolipidemic nutrient ingredients applied in public healthy and functional foods.

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Purification and Characterization of Antioxidant Peptides Derived from Protein Hydrolysate of the Marine Bivalve Mollusk Tergillarca granosa

Cited by 60 publications

References 55 publications

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Bioactive Peptides from Marine Molluscs – A Review

Hypolipidemic Activities of Two Pentapeptides (VIAPW and IRWWW) from Miiuy Croaker (Miichthys miiuy) Muscle on Lipid Accumulation in HepG2 Cells through Regulation of AMPK Pathway

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