Purification and Characterization of Antioxidant Peptides from Oyster (Saccostrea cucullata) Hydrolysate and the Anticancer Activity of Hydrolysate on Human Colon Cancer Cell Lines

Umayaparvathi, S.; Muthuvel, A.; Selvaraju, M.; Dräger, Gerald; Kirschning, Andreas; Balasubramanian, T.

doi:10.1007/s10989-013-9385-5

Cited by 27 publications

(25 citation statements)

References 34 publications

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“…Recently, several studies have been focused on the generation of bioactive peptides from food proteins and their utilization as functional ingredients [23]. Previous studies revealed that oyster is a good source of biologically active peptides with antioxidant, anti-cancer, ACE inhibitory, and anti-microbial activities in vitro and show antihypertensive activity in vivo [24,25,26,27]. Having these therapeutic potential, oysters can be considered as an alternative source of peptides that can be used as an ingredient for functional foods and nutraceuticals.…”

Section: Introductionmentioning

confidence: 99%

In Silico and In Vitro Assessment of Portuguese Oyster (Crassostrea angulata) Proteins as Precursor of Bioactive Peptides

Gomez

Peralta

Tejano

et al. 2019

IJMS

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In this study, the potential bioactivities of Portuguese oyster (Crassostrea angulata) proteins were predicted through in silico analyses and confirmed by in vitro tests. C. angulata proteins were characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and identified by proteomics techniques. Hydrolysis simulation by BIOPEP-UWM database revealed that pepsin (pH > 2) can theoretically release greatest amount of bioactive peptides from C. angulata proteins, predominantly angiotensin I-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) inhibitory peptides, followed by stem bromelain and papain. Hydrolysates produced by pepsin, bromelain and papain have shown ACE and DPP-IV inhibitory activities in vitro, with pepsin hydrolysate (PEH) having the strongest activity of 78.18% and 44.34% at 2 mg/mL, respectively. Bioactivity assays of PEH fractions showed that low molecular weight (MW) fractions possessed stronger inhibitory activity than crude hydrolysate. Overall, in vitro analysis results corresponded with in silico predictions. Current findings suggest that in silico analysis is a rapid method to predict bioactive peptides in food proteins and determine suitable enzymes for hydrolysis. Moreover, C. angulata proteins can be a potential source of peptides with pharmaceutical and nutraceutical application.

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Section: Introductionmentioning

confidence: 99%

In Silico and In Vitro Assessment of Portuguese Oyster (Crassostrea angulata) Proteins as Precursor of Bioactive Peptides

Gomez

Peralta

Tejano

et al. 2019

IJMS

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“…Recently, peptides with potential antioxidant activity have been obtained from various materials, such as soy proteins [7,8], caseins [9,10], egg proteins [11], aquatic product proteins [12][13][14], and other materials.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antioxidant Activity of Peptides from Simulated Gastro-Intestinal Digestion Products of Cyprinus carpio haematopterus Scale Gelatin

Xiao

Chen

et al. 2019

Foods

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A two-stage simulated gastro-intestinal (GI) digestion model (2 h pepsin treatment and subsequent 2 h pancreatin treatment at 37 °C) was used to explore the antioxidant activity of the digested products of Cyprinus carpio haematopterus scale gelatin with different molecular weights (MW). From the gastric phase to the intestinal phase, the hydrolysis degree of the products increased from 2.6 ± 0.4% to 16.9 ± 0.7%. The fraction of 0–1 kDa (JCP3) exhibited the best antioxidant activities in hydroxyl radical scavenging, reducing power, and metal chelating activity. The fraction of 1–3 kDa (JCP2) exhibited the best 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. However, the fractions of 0–1 kDa (JCP3) and 1–3 kDa (JCP2) showed similar inhibitory activity of lipid peroxidation. The results indicated that Cyprinus carpio haematopterus scale gelatin can be digested in the gastrointestinal tract. Furthermore, the digested products had antioxidant activity.

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“…Anti‐oxidant substances, such as flavonoids and isoflavones, can improve testicular steroidogenesis by upregulating StAR expression, which can prevent late‐onset male hypogonadism 33 . However, the peptides obtained by enzymatic hydrolysis of oyster have a good scavenging effect on potent 1,1‐diphenyl‐2‐picrylhydrazyl free radicals 34,35 . The results of the present study showed that low‐dose CP could significantly decrease GSH‐px, but increase MDA levels in testis, suggesting that during PADAM formation, the ability of scavenging ROS in testis is weakened, and the generation of free radicals is increased, which results in the damage of seminiferous tubules and interstitial cells.…”

Section: Discussionmentioning

confidence: 61%

Oyster oligopeptide improving cyclophosphamide‐induced partial androgen deficiency of the aging male by promotion of testosterone synthesis

Qi-guan

Shi

et al. 2021

Geriatrics Gerontology Int

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Aim The aim of this study was to investigate the effect of oyster oligopeptide (OOP) at different doses on testosterone secretion and its regulating mechanism in partial androgen deficiency syndrome of aging male. Methods The cyclophosphamide‐induced partial androgen deficiency syndrome of the aging male rats were treated with a low, medium and high dose of OOP for 6 weeks. Results Cyclophosphamide could decrease levels of total testosterone and luteinizing hormone in serum, and testosterone and glutathione peroxidase in testis, and increase malondialdehyde, and downregulate the mRNA expression of steroidogenic acute regulatory protein, steroidogenic acute regulatory‐related lipid transfer domain 7 and P450 cholesterol side chain cleavage enzyme in testis (P < 0.05). All these changes were reversed by OOP co‐administration with different doses, although, OOP at a low dose did not increase serum testosterone, luteinizing hormone and testicular glutathione peroxidase levels. Conclusions OOP treatment with different doses can effectively reduce oxidative stress in testicular tissue, promote the synthesis of testosterone and then prevent the formation of partial androgen deficiency syndrome of the aging male, with optimal effect at medium dose. Geriatr Gerontol Int 2021; 21: 268–275.

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Purification and Characterization of Antioxidant Peptides from Oyster (Saccostrea cucullata) Hydrolysate and the Anticancer Activity of Hydrolysate on Human Colon Cancer Cell Lines

Cited by 27 publications

References 34 publications

In Silico and In Vitro Assessment of Portuguese Oyster (Crassostrea angulata) Proteins as Precursor of Bioactive Peptides

In Silico and In Vitro Assessment of Portuguese Oyster (Crassostrea angulata) Proteins as Precursor of Bioactive Peptides

In Vitro Antioxidant Activity of Peptides from Simulated Gastro-Intestinal Digestion Products of Cyprinus carpio haematopterus Scale Gelatin

Oyster oligopeptide improving cyclophosphamide‐induced partial androgen deficiency of the aging male by promotion of testosterone synthesis

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