Purification and characterization of aminopeptidase N from chicken intestine with potential application in debittering

Purification and characterization of proline aminopeptidase from chicken intestine

“…We have already reported a process to debitter protein hydrolysates using chicken intestinal mucosa [6]. Moreover, two different types of alanine aminopeptidases from this source have already been purified and characterized [7,15] and involvement of one of these in degradation of bitter peptides of casein hydrolysate has been demonstrated [7]. In the present paper, we have reported purification and characterization of proline specific aminopeptidase of chicken intestine, which also could be used as a potential debittering enzyme.…”

“…For instance, treatment of exopeptidases rich enzyme preparations, particularly peptidases, which cleave adjacent to hydrophobic amino acid residues, during concomitant or subsequent to the proteinase digest of food, could significantly reduce the bitterness of the preparations. Hence, aminopeptidases from various sources are successfully used to debitter protein hydrolysates [4][5][6] and a number of other from different sources have been suggested for this application [7][8][9]. However, much research has been focused on the application of proline specific exopeptidases in the hydrolysate debittering strategies due to the inability of most general aminopeptidases to hydrolyze the imino bond and a unique contribution of Pro-residues in the bitterness of protein hydrolysates [10].…”

Proteolytic activity from chicken intestine and pancreas: extraction, partial characterization and application for hyaluronic acid separation from chicken comb

“…These enzymes were found to be concentrated in the mucosa of chicken intestine. The proteolytic enzyme aminopeptidase N extracted from chicken intestine has been applied in the debittering of protein hydrolysate …”

Characterization of Activity of a Potential Food-Grade Leucine Aminopeptidase from Kiwifruit

“…Animal waste products were also investigated as a potential source of useful APs [5]. It is also possible that APs from plants could be of use in the food processing industry [6].…”