Purification and characterization of a peptide from soybean with cancer cell proliferation inhibition

Abstract

A peptide from protein hydrolysate fraction obtained from a high oleic acid soybean line (N98‐4445A) that had shown significant activity against human cancer cell lines was identified and purified. Three peptides showing highest activity were identified by reverse phase HPLC in the 10–50 kDa fraction of the protein and purified using peptide specific affinity chromatography column. The three individual peptides were tested for anti‐proliferative activity against blood, colon and liver cancer cell lines using 3‐(4,5‐dimethyl thiazole‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) cell titer assay. Enhanced colon cancer cell inhibition (80%) was observed after testing a pure peptide (molecular size of 18 kDa with 158 amino acid residues) which also demonstrated a time‐response of 96 hr after incubation based on trypan blue dye exclusion study. The impact of this study lies in deriving a pure single peptide with anti‐proliferative activity on human colon and blood cancer cells. Practical applications Soybean is cultivated in the United States mostly for extracting the oil leaving the residue (soybean meal) which is commonly used as an animal feed due to its high protein content. This study has shown that soy peptides produced enzymatically from the meal of a high oleic acid soybean line (N98‐4445A) were found to have anticancer activity. This study demonstrated an impending value for a pure peptide derived from this soy peptides as an alternative and inexpensive anti‐cancer therapeutic agent as well as a value addition to soy meal by‐product.

Novel non-specific lipid-Transfer Protein (TdLTP4) isolated from Durum wheat: Antimicrobial activities to control pathogen and spoilage bacteria and Anti-Inflammatory properties in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages

“…In the current study, TdLTP4 signi cantly increased the levels of antiin ammatory cytokine IL-10. These results are in concordance with those of Rayaprolu et al (2017) who reported that IL-6 and TNF-α production was considerably increased in LPS-stimulated RAW 264.7 cells but signi cantly inhibited by Hazelnut protein-derived peptide LDAPGHR in a dose-dependent manner . Sangtanoo et al (2020) reported that Peanut worm (Sipunculus nudus Linn.)…”

Docosahexaenoic acid‐containing phosphatidylcholine induced osteoblastic differentiation by modulating key transcription factors

“…Then, the cells were treated with a medium containing DHA‐PC (0, 12.5, 25, 50, 100, and 200 μg/ml). After incubation for 24, 48, 72 or 96 hr, the cells were incubated with thiazolyl blue tetrazolium bromide (MTT) solution (0.5 mg/ml) and cell proliferation was assessed using the MTT assay, as described previously (Rayaprolu et al, ).…”

Preparation and Evaluation of Antioxidant Activities of Bioactive Peptides Obtained from Cornus officinalis

“…The effects of different concentrations of Cornus officinalis peptides on CEF cell proliferation were tested by MTT method [38]. Briefly, CEF cells in the logarithmic phase were inoculated into 96-well plates at 8 × 10 −3 density and 100 µL of different concentrations of Cornus officinalis peptides were added.…”