Preparation and Evaluation of Antioxidant Activities of Bioactive Peptides Obtained from Cornus officinalis

Abstract

The present study is a preparation of bioactive peptides from Cornus officinalis proteins by the compound enzymatic hydrolysis method. Response surface methodology (RSM) coupled with Box–Behnken design (BBD) is used to optimize the preparation process of Cornus officinalis peptides. The effects of independent variables, such as the amount of enzyme, pH value, time, extraction times and the ratio of material to liquid on the yield of peptides, are also investigated. The analysis results of the RSM model show that the optimum conditions for the extraction of Cornus officinalis peptides were a pH value of 6.76, temperature of 48.84 °C and the amount of enzyme of 0.19%. Under optimal conditions, the yield of peptides was 36.18 ± 0.26 %, which was close to the predicted yield by the RSM model. Additionally, the prepared Cornus officinalis peptides showed significant antioxidant activity; the scavenging rates of the peptides for DPPH and ·OH were 48.47% and 29.41%, respectively. The results of the cell proliferation assay revealed that the prepared Cornus officinalis peptides could promote embryo fibroblast cells proliferation and repair oxidative damage cells. These results have a practical application value in the design of novel functional food formulations by using Cornus officinalis.

Modulation of cecal microbiota and fecal metabolism in mice by walnut protein

“…4 As a high-quality protein, the utilization rate of walnut protein is extremely low in China, and the protein content of walnut meal is up to 40% or more, from which the extraction of walnut protein can significantly increase the added value of the walnut industry. 5 Numerous studies have shown that walnut protein has significant antioxidant, 6 anti-inflammatory, 7 blood pressure-lowering, 8 and sleep-improvement 9 effects. In addition, according to Elouafy et al , 10 pyruvic acid, which is present in walnuts, has the ability to perform functions of dissolution, abatement and excretion, which has a good curative effect on gallstones; walnuts are rich in vitamin E, which has a function in cosmetology.…”