1997
DOI: 10.1021/jf9607674
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Purification and Characterization of a Polyphenol Oxidase from the Kew Cultivar of Indian Pineapple Fruit

Abstract: Three isoforms of polyphenol oxidase (PPO) were purified to apparent homogeneity from the Kew cultivar of Indian pineapple fruit in a four-step procedure. The major isoenzyme, with a yield of 45%, was found to be a tetramer of identical subunits of molecular mass ≈25 kDa. An ionic strength dependent association−dissociation equilibrium was observed with pineapple PPO. Amino acid analysis of the major isoenzyme indicated the presence of a high content of glutamic acid, glycine, and serine and a low content of t… Show more

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Cited by 83 publications
(63 citation statements)
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“…We can also observe that when there is an ammonium sulphate saturation concentration of 80% (Table 1), the sapodilla plum's PPO precipitation is almost total, due to the fact that under these conditions a purification factor of approximately 4.8 was obtained, and, therefore, a specific activity factor of 762.7 UE factor was found. On the other hand, a recovery factor of almost 3 was reported by Jharna R. [23] in the purification and characterization of the pineapple's PPO, and a similar value of 4.5 was obtained in the partial purification of persimmon by Nuñez, Sojo, García and Sánchez [24].…”
Section: Enrichment Of the Sapodilla Plum's Ppo Enzymatic Extractsupporting
confidence: 60%
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“…We can also observe that when there is an ammonium sulphate saturation concentration of 80% (Table 1), the sapodilla plum's PPO precipitation is almost total, due to the fact that under these conditions a purification factor of approximately 4.8 was obtained, and, therefore, a specific activity factor of 762.7 UE factor was found. On the other hand, a recovery factor of almost 3 was reported by Jharna R. [23] in the purification and characterization of the pineapple's PPO, and a similar value of 4.5 was obtained in the partial purification of persimmon by Nuñez, Sojo, García and Sánchez [24].…”
Section: Enrichment Of the Sapodilla Plum's Ppo Enzymatic Extractsupporting
confidence: 60%
“…This suggests that the denaturalized molecular weight of the sapodilla plum's PPO is close to 29 kDa. The results found by Jharna R. [23] in the purification and characterization of the Indian pineapple's PPO showed that the molecular weight was 105 kDa with a Sephadex G-150 column; however, the SDS-PAGE gel indicated a single 25 kDa polypeptide band, suggesting a tetramer of identical units. Applying this principle to the sapodilla plum's PPO, we think that, in this case, the polypeptide is a dimmer of identical units.…”
Section: Electrophoresis Of the Purified Fractions (Phenylsepharose Amentioning
confidence: 93%
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“…In the earlier studies Kavrayan and Aydemir (2001), Siddiq and Cash (2000), Paul and Gowda (2000), Das et al (1997) reported sodium metabisulfite as the most effective inhibitor against peppermint, pears, field bean and pineapple fruit PPO, respectively. A strong inhibitory effect of SO 2 on soursop PPO at pH 7.5 was also observed in our study.…”
Section: Metabisulphitementioning
confidence: 97%
“…Este resultado concorda com o obtido por DAS et al (1997), que não detectaram atividade de polifenoloxidase no extrato bruto de abacaxi.…”
Section: Determinação Da Atividade Da Polifenoloxidaseunclassified