Purification and Characterization of a Xylanase Produced by Chaetomium thermophile NIBGE

Latif, Farooq; Asgher, Muhammad; Saleem, Rabia; Akrem, Ahmed; Legge, Raymond L.

doi:10.1007/s11274-005-5745-4

Cited by 21 publications

(10 citation statements)

References 34 publications

(37 reference statements)

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“…The optimum pH of purified xylanase was found to be 6.0 and further rise in pH was resulted in decreased the activity (Figure 15) and these findings were concordant to previously study in which Aspergillusterreus showedoptimum activity at pH 6 [18], while Chaetomium thermophile was 6.5 [20] and purified xylanase of Paecilomycesthermophila had optimum activity pH of 7.0. The enzyme was stable over a broad range pH 6.0 -11.0 [10].…”

Section: Characterization Of рH On Purified Xylanasesupporting

confidence: 90%

Screening and Statistical Optimization of Physiochemical Parameters for the Production of Xylanases from Agro-Industrial Wastes

Ali

Irshad

Anwar

et al. 2016

AER

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Xylanases are mostly produced through submerged fermentation; nonetheless solid-state fermentation has increased profound attention and consideration of scholars having high conversion level biomass to energy conservation. This study depicted the purification of xylanases and their possible utilization in industry. The present study was carried out to examine the culture influence of fungal strain Fomes fomentarius (F. fomentarius) using different agro-industrial residues (wheat straw, rice husk, sugarcane bagasse and siris pods). F. fomentarius showed maximum enzyme production after 72 h of fermentation, when grown on wheat straw in solid state fermentation process while maximum activity showed on pH 6.0 at 30˚C. The other parameters optimized by statistical design (RSM) showed maximum xylanase activity (146 ± 8 IU/mL) at 65% moisture content, 4 mL inoculums size, 175 mg Ammonium sulphate, 200 mg Calcium carbonate and 1.4 grams of glucose. Xylanase was salted out at 60% ammonium sulphate concentration and enzyme was further purified by Sephadex G-100 gel filtration chromatography with 2.2 fold increase in activity. The purified xylanase from F. fomentarius had optimum pH 6.0 and 40˚C. Xylanase showed higher specificity for oat spelt xylan with kinetic constants Km 1.25 mg/mL and Vmax 54 mM/min. Xylanases have an industrial important enzyme used extensively in food, feed and paper industry.

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Section: Characterization Of рH On Purified Xylanasesupporting

confidence: 90%

Screening and Statistical Optimization of Physiochemical Parameters for the Production of Xylanases from Agro-Industrial Wastes

Ali

Irshad

Anwar

et al. 2016

AER

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“…Trichosporum cutaneum [14], Alternaria sp. ND-16 [15], Chaetomium thermophile [16], and Aspergillus tamarii [17], when cultivated on submerged fermentation using wheat bran as carbon source, presented xylanase activity of 39.7 U mL −1 , 20.0 U mL −1 , 27.8 U mL −1 , and 54.32 U mL −1 , respectively. Better results of xylanase activity of 107 U mL −1 , 102 U mL − 1, and 97 U mL −1 were obtained when Thermoascus aurantiacus was cultivated on solid-state fermentation using corncob, grass, and corn straw, respectively [9].…”

Section: Solid-state Fermentationmentioning

confidence: 99%

“…On the other hand, some authors use agroindustrial waste for submerged fermentation processes for cultivating bacteria or fungi, such as Bacillus circulans AB16 [11], Clostridium absonum CFR-702 [12], Aspergillus foetidus MTCC4898 [13], Trichosporon cutaneum SL409 [14], Alternaria sp. ND-16 [15], Chaetomium thermophile NIBGE [16], Aspergillus tamari [17]. Other authors use solidstate fermentation in minor scale for preferentially cultivating fungi, such as Thermoascus aurantiacus [9,18], Trichoderma harzianum [19], Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Screening and Production Study of Microbial Xylanase Producers from Brazilian Cerrado

Alves-Prado

Pavezzi

Leite

et al. 2009

Appl Biochem Biotechnol

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Hemicelluloses are polysaccharides of low molecular weight containing 100 to 200 glycosidic residues. In plants, the xylans or the hemicelluloses are situated between the lignin and the collection of cellulose fibers underneath. The xylan is the most common hemicellulosic polysaccharide in cell walls of land plants, comprising a backbone of xylose residues linked by beta-1,4-glycosidic bonds. So, xylanolytic enzymes from microorganism have attracted a great deal of attention in the last decade, particularly because of their biotechnological characteristics in various industrial processes, related to food, feed, ethanol, pulp, and paper industries. A microbial screening of xylanase producer was carried out in Brazilian Cerrado area in Selviria city, Mato Grosso do Sul State, Brazil. About 50 bacterial strains and 15 fungal strains were isolated from soil sample at 35 degrees C. Between these isolated microorganisms, a bacterium Lysinibacillus sp. and a fungus Neosartorya spinosa as good xylanase producers were identified. Based on identification processes, Lysinibacillus sp. is a new species and the xylanase production by this bacterial genus was not reported yet. Similarly, it has not reported about xylanase production from N. spinosa. The bacterial strain P5B1 identified as Lysinibacillus sp. was cultivated on submerged fermentation using as substrate xylan, wheat bran, corn straw, corncob, and sugar cane bagasse. Corn straw and wheat bran show a good xylanase activity after 72 h of fermentation. A fungus identified as N. spinosa (strain P2D16) was cultivated on solid-state fermentation using as substrate source wheat bran, wheat bran plus sawdust, corn straw, corncob, cassava bran, and sugar cane bagasse. Wheat bran and corncobs show the better xylanase production after 72 h of fermentation. Both crude xylanases were characterized and a bacterial xylanase shows optimum pH for enzyme activity at 6.0, whereas a fungal xylanase has optimum pH at 5.0-5.5. They were stable in the pH range 5.0-10.0 and 5.5-8.5 for bacterial and fungal xylanase, respectively. The optimum temperatures were 55 and 60 degrees C for bacterial and fungal xylanase, respectively, and they were thermally stable up to 50 degrees C.

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“…Therefore, enzyme production now became a multi-billion dollar business (Bhat 2000). Some species of Chaetomium produce several enzymes (Fähnrich & Irrgang 1982;Markham & Bazin 1991;Czakaj & Czuba 2003;El-Gindy et al 2003;Latif et al 2006;El-Zayat 2008;Abdel-Azeem & Salem 2012) through their activity. Although many consortia of fungi have been developed in laboratories for the degradation of complex materials, still there is a demand for new mycobiota which can secrete large amount of hydrolytic enzymes to decompose different substrates.…”

Section: Introductionmentioning

confidence: 99%

Enzyme profiles and genotyping ofChaetomium globosumisolates from various substrates

Abdel-Azeem

Gherbawy

Sabry

2014

Plant Biosystems - An International Journal Dealing with all As

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The genus Chaetomium is a rich source of novel and bioactive secondary metabolites of great importance. To date, a variety of more than 200 secondary metabolites belonging to diverse structural types have been discovered. Fungal enzymes are used in food, beverages, confectionaries, textiles, and leather industries to simplify the processing of raw materials. They are often more stable than enzymes derived from other sources. Ten isolates of Chaetomium globosum recovered and designated as TUCg1 to TUCg10 were identified by morphological and molecular biology means and submitted to the GenBank. These isolates were screened for extracellular enzymes such as amylase, cellulase, laccase, lipase, pectinases, protease and chitinase on solid media. All Chaetomium globosum isolates screened for potential enzymes showed amylolytic, cellulolytic, and proteolytic activities; six isolates were chitinolytic and laccase producers; and five and three isolates showed pectinolytic and lipolytic activities, respectively. The produced array of enzymes differed among isolates. Molecular techniques such as internal transcribed spacer (ITS) region sequencing and specific genes random primers polymerase chain reaction (SGRP-PCR) have shown high DNA polymorphism of Chaetomium globosum. In conclusion, SGRP-PCR is a rapid and valuable tool for assessment and characterization of genetic diversity of Chaetomium globosum, which suggests the use of this technique for identification of different fungal isolates.

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Purification and Characterization of a Xylanase Produced by Chaetomium thermophile NIBGE

Cited by 21 publications

References 34 publications

Screening and Statistical Optimization of Physiochemical Parameters for the Production of Xylanases from Agro-Industrial Wastes

Screening and Statistical Optimization of Physiochemical Parameters for the Production of Xylanases from Agro-Industrial Wastes

Screening and Production Study of Microbial Xylanase Producers from Brazilian Cerrado

Enzyme profiles and genotyping ofChaetomium globosumisolates from various substrates

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