Purification and characterization of a cadmium-binding protein from Nereis diversicolor (Annelida, polychaeta)

Nejmeddine, A.; Dhainaut-Courtois, N.; Baert, Jean-Luc; Sáutière, Pierre; Fournet, Bernard; Boulenguer, Patrick

doi:10.1016/0742-8413(88)90231-9

Cited by 24 publications

(18 citation statements)

References 11 publications

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“…The chromatographic profile observed at 280·nm (Fig.·lA) revealed the presence of three peaks, a feature identical to that observed on analysis of whole worms exposed to Cd (Nejmeddine et al, 1988).…”

Section: Chromatography and Metal Analysissupporting

confidence: 52%

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Stimulation by cadmium of myohemerythrin-like cells in the gut of the annelidNereis diversicolor

Demuynck¹,

Bocquet-Muchembled²,

Deloffre³

et al. 2004

Journal of Experimental Biology

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Trace metals, whether essential such as Cu and Zn or nonessential such as Cd, are taken up by aquatic invertebrates from both food and solutions. After ingestion, the metal is transported around the body, perhaps to be accumulated in particular target organs or even to be excreted (Rainbow, 1998). In most invertebrates, body concentrations of the non-essential metal Cd do not appear to be regulated (Rainbow, 1985;Amiard et al., 1987;Rainbow, 1998). Metal toxicity is often postulated to arise from reactions occurring in the cytosol, through nonspecific binding of the metal to non-thionein ligands, which are physiologically important molecules (metalloenzymes or small peptides such as glutathione) and are inactivated by metal binding (Mason and Jenkins, 1995). Control of intracellular metal toxicity and detoxification of accumulated trace metals is generally achieved via the production of metal-binding ligands that sequester metals. These cellular ligands can be found in the particulate fraction of the cells such as mineral deposits, granules or lysosomes (George, 1990;Mason and Jenkins, 1995) and in the cytosol such as metallothioneins (Engel and Roesijadi, 1987). Metallothioneins, a family of low molecular mass cysteine-rich proteins, have been shown to occur in most zoological taxa (Amiard and Cosson, 1997). However, in polychaetous annelids this type of protein has not yet been isolated.Nereis diversicolor (Hediste diversicolor, recent denomination) is a polychaete living in the mud of estuaries which, in Europe, is routinely contaminated by heavy metals. Previous work (Nejmeddine et al., 1988) showed that in animals exposed to Cd the metal was bound to two pools of proteins of high molecular mass (metalloprotein I, MPI, >67·kDa) and low molecular mass (MPII, of about 20·kDa). MPI is the extracellular hemoglobin of this annelid (Demuynck and Dhainaut-Courtois, 1993). The primary structure of MPII isolated from whole worms was different from that of a metallothionein, consisting of 119 amino acid residues with only one cysteine residue , but shares 80.8% identity with the myohemerythrin isolated by Takagi and Cox (1991) from the same species. MPII is a Cd-binding protein but is not a metallothionein. Metallothioneins are known to be induced by essential (Cu or Zn) and non-essential (Cd) metals and are thought to be important both in the detoxification of trace metals and in the metabolism of intracellular Cu and Zn, as in the scavenging of reactive oxygen species (Palmiter, 1998;Klaassen et al., 1999). However, MPII appeared to correspond to a myohemerythrinlike pigment, known to play a mainly respiratory role and with no known function of detoxification. Immunocytochemical studies showed that MPII is located in granulocytes I Isolated guts of Nereis diversicolor revealed the existence of a cadmium-binding protein, the MPII, belonging to the group of hemerythrins and myohemerythrins. The presence of MPII in the cells of the intestine was demonstrated by immunocytochemistry, using anti-MPII, a monoclonal antibod...

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Section: Chromatography and Metal Analysissupporting

confidence: 52%

“…Previous work (Nejmeddine et al, 1988) showed that in animals exposed to Cd the metal was bound to two pools of proteins of high molecular mass (metalloprotein I, MPI, >67·kDa) and low molecular mass (MPII, of about 20·kDa). MPI is the extracellular hemoglobin of this annelid (Demuynck and Dhainaut-Courtois, 1993).…”

mentioning

confidence: 99%

Stimulation by cadmium of myohemerythrin-like cells in the gut of the annelidNereis diversicolor

Demuynck¹,

Bocquet-Muchembled²,

Deloffre³

et al. 2004

Journal of Experimental Biology

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“…In some works, there is information on the mechanisms of heavy metal detoxification in small-sized organisms of the infauna; but it is merely primary. Thus, the resistance of N. diversicolor has been linked to low absorption of metals due to com- plexing in the epidermal slime, isolation of toxicants into spheral crystals in the intestinal tract, and by the binding of Cd to metallothionein-like proteins [25].…”

Section: Resultsmentioning

confidence: 99%

Heavy Metal Contents in Tissues of Dominant Species of the Benthos and in Bottom Sediments of Zolotoi Rog Bay, Sea of Japan

et al. 2005

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Municipal and anthropogenic inputs into the ports of Peter the Great Bay have led to foci of local-and regional-scale pollution where the natural course of biological processes is disturbed [3]. Here, the content of heavy metals in the bottom sediments, pore water, and near-bottom water is far higher than in the water column [17,28]. High environmental levels of heavy metals cause metal accumulation by aquatic organisms [13].Study of heavy metal accumulation by bottom organisms and metal toxicity in the biota is part of the study of pollution effects on aquatic ecosystems. Up to now, the interaction of bottom biota and pollutants (particularly heavy metals) has largely been examined for microorganisms [12,15], phyto-and zooplankton [7], and seston-feeding [8,15] and fouling organisms [4,5]. Small representatives of the infauna have not been investigated [1,28] in this respect. Polychaetes and round worms are the major functional components of the biota in the bottom sediments. They can build up a significant biomass and play an important role in the transformation of organic compounds of the sea bottom [7,18]. However, the mechanism of the effect of pollution on the metabolism and numbers of marine bottom communities is inadequately known.The aim of this study was to determine the heavy metals contents in the tissues of dominant species of the benthos and in the bottom sediments of chronically polluted areas. MATERIALS AND METHODSSamples of silt sediments were collected in 2000-2003 in Zolotoi Rog Bay (Sea of Japan) near the port of Vladivostok from the 13 m depth at Wharf no. 44. Animals were collected from samples in Zolotoi Rog Bay and Vostok Bay in 2003.Sediment samples were washed with seawater through gauze (mesh size 0.1 × 0.1 mm). Sediment was placed in petri dishes and examined, by parts, under a stereomicroscope for the presence of bottom worm. Worms were isolated and sorted into species. Two to 100 specimens (lengths of 9-100 mm) of each species were taken for heavy metal analyses. To remove sediment particles, the worms were washed with seawater and then placed for 1-4 days in separate vessels with filtered seawater to purge the intestine.For each species, the measurements of the heavy metal concentrations were made in three samples with the number of worms depending on their size. For atomic absorption analysis, worm samples were prepared using the method of acid mineralization according to standard procedure GOST 26929-94 [2]. Wet tis-ECOLOGY Abstract -Data on heavy metal contents in polychaetes and free-living nematodes inhabiting the bottom sediments of Zolotoi Rog Bay near the port of Vladivostok are reported. Chronically high contents of heavy metals (Fe, Cu, Zn, and Cd) were found in the bottom sediments and in the infauna. The levels of some toxic elements in tissues of the polychaete Dorvillea (Schistomeringos) japonica inhabiting polluted sediments of Zolotoi Rog Bay were higher than in worms of this species from relatively clean areas of the Sea of Japan. Similar high concentrations of Fe w...

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“…Some species have been shown to concentrate cadmium by specific binding proteins with antibacterial activity (Nejmeddine et al 1988;Sandrini et al 2006), while elevated levels of copper or zinc halogens in nereids jaws have been related to both a structural function and a first defense against bacteria (Lichtenegger et al 2002).…”

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confidence: 99%

Hyperaccumulation of vanadium in the Antarctic polychaete Perkinsiana littoralis as a natural chemical defense against predation

Fattorini

Notti

Nigro

et al. 2009

Environ Sci Pollut Res

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The hyperaccumulation of toxic metals might represent a common antipredatory strategy for unpalatable branchial crowns of sabellid polychaetes, as recently hypothesized also for the high concentrations of arsenic in the Mediterranean Sabella spallanzanii. The evolution of such adaptation and the reasons behind the possibility for different species to accumulate different metals represent a stimulating field of investigation for future studies.

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Purification and characterization of a cadmium-binding protein from Nereis diversicolor (Annelida, polychaeta)

Cited by 24 publications

References 11 publications

Stimulation by cadmium of myohemerythrin-like cells in the gut of the annelidNereis diversicolor

Stimulation by cadmium of myohemerythrin-like cells in the gut of the annelidNereis diversicolor

Heavy Metal Contents in Tissues of Dominant Species of the Benthos and in Bottom Sediments of Zolotoi Rog Bay, Sea of Japan

Hyperaccumulation of vanadium in the Antarctic polychaete Perkinsiana littoralis as a natural chemical defense against predation

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