Purification and characterization of a phosphoric triester hydrolase from the tufted apple bud moth,Platynota idaeusalis (Walker)

Devorshak, Christina; Roe, R. Michael

doi:10.1002/1099-0461(2001)15:1<55::aid-jbt7>3.0.co;2-g

Cited by 2 publications

(2 citation statements)

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“…Both of these enzymes metabolize OPs, including esters of phosphonic and phosphinic acids, and those with phosphorus anhydride bonds (IUBMB, ). The assay is a modification of a microtiter plate procedure developed by Devorshak and Roe (), which is based on a macroassay described by Furlong et al (). The substrate is methyl paraoxon, which when metabolized by PTEHs, yields the yellow‐colored product, p ‐nitrophenol.…”

Section: Commentarymentioning

confidence: 99%

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Assays for the Classification of Two Types of Esterases: Carboxylic Ester Hydrolases and Phosphoric Triester Hydrolases

Anspaugh

Roe

2002

CP Toxicology

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Assays for the Classification of Two Types of Esterases: Carboxylic Ester Hydrolase and Phosphoric Triester Hydrolase (Douglas D. Anspaugh and Michael Roe, North Carolina State University, Raleigh, North Carolina). This unit describes assays that quantitate two types of esterase the carboxylic ester hydrolases and the phosphoric triester hydrolases. Carboxylic ester hydrolases include the B-esterases, which are inhibited by organophosphorus compounds. Among the phosphoric triester hydrolases is aryldialkylphosphatase, which has been called A-esterase or paraoxonase due to its ability to oxidize paraoxon and other organophosphates. These assays are colorimetric and miniaturized for rapid simultaneous testing of multiple, small-volume samples in a microtiter plate format. There is also a discussion of the history of esterase nomenclature and the reasons why this large group of enzymes is so difficult to classify.

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Section: Commentarymentioning

confidence: 99%

“…Several inhibitors and activators of PTEH activity have been documented (Aldridge, ; Shishido and Fukami, ; Konno et al, ; Devorshak and Roe, ). Perhaps the most effective PTEH inhibitors are mercuric compounds such as p ‐chloromercuribenzoate (PCMB).…”

Section: Commentarymentioning

confidence: 99%