Purification and characterization of a novel caffeine oxidase from Alcaligenes species

Mohapatra, Bhagyalaxmi; Harris, Norman F.; Nordin, Rick; Mazumder, Arindam Ghosh

doi:10.1016/j.jbiotec.2006.03.018

Cited by 48 publications

(37 citation statements)

References 24 publications

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“…Some bacteria utilize caffeine as sole growth substrate and metabolize it via oxidation at the C-8 position to form 1,3, 7-trimethyluric acid (Madyastha & Sridhar, 1998;Mohapatra et al, 2006;Yu et al, 2008). This reaction is catalysed by a number of different enzymes in several bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source

Summers

Louie

et al. 2011

Microbiology

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N-Demethylation of many xenobiotics and naturally occurring purine alkaloids such as caffeine and theobromine is primarily catalysed in higher organisms, ranging from fungi to mammals, by the well-studied membrane-associated cytochrome P450s. In contrast, there is no well-characterized enzyme for N-demethylation of purine alkaloids from bacteria, despite several reports on their utilization as sole source of carbon and nitrogen. Here, we provide what we believe to be the first detailed characterization of a purified N-demethylase from Pseudomonas putida CBB5. The soluble N-demethylase holoenzyme is composed of two components, a reductase component with cytochrome c reductase activity (Ccr) and a two-subunit N-demethylase component (Ndm). Ndm, with a native molecular mass of 240 kDa, is composed of NdmA (40 kDa) and NdmB (35 kDa). Ccr transfers reducing equivalents from NAD(P)H to Ndm, which catalyses an oxygen-dependent N-demethylation of methylxanthines to xanthine, formaldehyde and water. Paraxanthine and 7-methylxanthine were determined to be the best substrates, with apparent K m and kcat values of 50.4±6.8 μM and 16.2±0.6 min−1, and 63.8±7.5 μM and 94.8±3.0 min−1, respectively. Ndm also displayed activity towards caffeine, theobromine, theophylline and 3-methylxanthine, all of which are growth substrates for this organism. Ndm was deduced to be a Rieske [2Fe–2S]-domain-containing non-haem iron oxygenase based on (i) its distinct absorption spectrum and (ii) significant identity of the N-terminal sequences of NdmA and NdmB with the gene product of an uncharacterized caffeine demethylase in P. putida IF-3 and a hypothetical protein in Janthinobacterium sp. Marseille, both predicted to be Rieske non-haem iron oxygenases.

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Section: Introductionmentioning

confidence: 99%

“…A 65 kDa caffeine oxidase was also purified from Alcaligenes sp. strain CF8 (Mohapatra et al, 2006). A heterotrimeric, non-NAD(P) + -dependent caffeine dehydrogenase from Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source

Summers

Louie

et al. 2011

Microbiology

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“…strain CF8 (65 kDa) and a Rhodococcus sp.-Klebsiella sp. mixed-culture consortium (85 kDa) (11,13). However, the CBB1 caffeine dehydrogenase subunit molecular masses and ␣␤␥ subunit structure are similar to those of Veillonella atypica (20).…”

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confidence: 97%

“…This property is unique. Oxygen is a poor electron acceptor for the two known bacterial caffeine oxidases (11,13), although they are defined as "oxidases." Oxygen is also the preferred electron acceptor for mammalian xanthine oxidases, while mammalian and bacterial xanthine dehydrogenases prefer NAD ϩ as the oxidant (8,9,19).…”

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confidence: 99%

A Novel Caffeine Dehydrogenase in Pseudomonas sp. Strain CBB1 Oxidizes Caffeine to Trimethyluric Acid

Kale

Gopishetty

et al. 2008

J Bacteriol

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A unique heterotrimeric caffeine dehydrogenase was purified from Pseudomonas sp. strain CBB1. This enzyme oxidized caffeine to trimethyluric acid stoichiometrically and hydrolytically, without producing hydrogen peroxide. The enzyme was not NAD(P) ؉ dependent; coenzyme Q 0 was the preferred electron acceptor. The enzyme was specific for caffeine and theobromine and showed no activity with xanthine.

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“…Caffeine (3,7-dihydro-1,3,7-trimethyl-1 h-purine-2,6-dione), a purine alkaloid, is the most commonly used legal drug in the world and has been added onto the US Environmental Protection Agency's list of high production volume of chemicals (Buerge et al 2003;Mohapatra et al 2006). In a recent review on the occurrence and fate of 137 micropollutants, Verlicchi and Zambello (2014) reported that caffeine has typically been detected in wastewater treatment plants (WWTPs) at concentrations ranging from 0.22 to 209 μg L −1 in influent and up to 43.5 μg L −1 in effluent.…”

Section: Introductionmentioning

confidence: 99%

Characterization of microbial communities in wetland mesocosms receiving caffeine-enriched wastewater

Zhang

Luo

Lee

et al. 2016

Environ Sci Pollut Res

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A 454 high-throughput pyrosequencing approach was used to characterize the structures of microbial communities in wetland mesocosms receiving caffeine-enriched wastewater at a concentration of 250 μg L −1. The removal efficiencies of caffeine in the planted beds (93.0 %) were significantly (p < 0.05) higher than those in the unplanted beds (81.4 %). Bacterial diversity was decreased by 25 and 22.4 %, respectively, in both planted and unplanted mesocosms after 210-day operation. The results of taxonomic analyses suggested that chronic exposure of wetland ecosystems to caffeine could lead to moderate shifts in microbial community composition. In total, 2156 operational taxonomic units (OTUs) were generated and 20 phyla comprising 260 genera were identified. The major phylogenetic groups at phylum level included Firmicutes (39 %), Actinobacteria (25.1 %), Proteobacteria (17.1 %), Synergistetes (5.6 %), and Chloroflexi (5.5 %).Bacilli and Synergistia increased in abundance in the planted mesocosms, while for the unplanted mesocosms, Actinobacterial, Clostridia and Betaproteobacteria exhibited increased proportion under the exposure of caffeine. At genus level, Propionibacterium, Staphylococcus, Bacillus, and Streptococcus were found to be increased in abundance after caffeine treatment. As for the response of fungal community to caffeine enrichment, genus like Cladosporium, Emericellopsis, Aspergillus, and Phoma were found to be resistant to caffeine disturbance. When compared to the microbial community between planted and unplanted mesocosms, a distinct community profile for both bacteria and fungi community was observed. The presence of plants had a remarkable effect on the structure of microbial community, helping buffer against the stress associated with caffeine exposure.

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Purification and characterization of a novel caffeine oxidase from Alcaligenes species

Cited by 48 publications

References 24 publications

Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source

Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source

A Novel Caffeine Dehydrogenase in Pseudomonas sp. Strain CBB1 Oxidizes Caffeine to Trimethyluric Acid

Characterization of microbial communities in wetland mesocosms receiving caffeine-enriched wastewater

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