Characterization of microbial communities in wetland mesocosms receiving caffeine-enriched wastewater

Abstract

A 454 high-throughput pyrosequencing approach was used to characterize the structures of microbial communities in wetland mesocosms receiving caffeine-enriched wastewater at a concentration of 250 μg L −1. The removal efficiencies of caffeine in the planted beds (93.0 %) were significantly (p < 0.05) higher than those in the unplanted beds (81.4 %). Bacterial diversity was decreased by 25 and 22.4 %, respectively, in both planted and unplanted mesocosms after 210-day operation. The results of taxonomic analyses suggested that chronic exposure of wetland ecosystems to caffeine could lead to moderate shifts in microbial community composition. In total, 2156 operational taxonomic units (OTUs) were generated and 20 phyla comprising 260 genera were identified. The major phylogenetic groups at phylum level included Firmicutes (39 %), Actinobacteria (25.1 %), Proteobacteria (17.1 %), Synergistetes (5.6 %), and Chloroflexi (5.5 %).Bacilli and Synergistia increased in abundance in the planted mesocosms, while for the unplanted mesocosms, Actinobacterial, Clostridia and Betaproteobacteria exhibited increased proportion under the exposure of caffeine. At genus level, Propionibacterium, Staphylococcus, Bacillus, and Streptococcus were found to be increased in abundance after caffeine treatment. As for the response of fungal community to caffeine enrichment, genus like Cladosporium, Emericellopsis, Aspergillus, and Phoma were found to be resistant to caffeine disturbance. When compared to the microbial community between planted and unplanted mesocosms, a distinct community profile for both bacteria and fungi community was observed. The presence of plants had a remarkable effect on the structure of microbial community, helping buffer against the stress associated with caffeine exposure.

Ecology and Biotechnological Potential of Bacteria Belonging to the Genus Pseudovibrio

“…Besides being found recurrently associated with various marine invertebrates, Pseudovibrio strains have been also detected in marine and estuarine sediments, seawater, oil samples, wastewater treatment systems, and wetland mesocosms ( Fig. 1 and 2) (1,2,(12)(13)(14)(15)(16)(17)(18). An overview of the sources where Pseudovibrio strains have been detected is shown in Fig.…”

Isolation of Cultivable Bacteria Associated with the Root of Typha latifolia in a Constructed Wetland for the Removal of Diclofenac or Naproxen

“…Regarding the removal of organic compounds, Zhang et al (2016) used T. angustifolia in an HSSF to remove caffeine (250 μg/L), obtaining a removal efficiency by 78.5% during the experimental period (7 months). The characterization of the bacterial consortium, using culture-independent methods, revealed that sediment is colonized by great bacterial diversity that could adapt to the wetland conditions and metabolizes the compound.…”

“…It has been demonstrated that S. maltophilia KB2 transforms naproxen, obtaining 28% of removal after 35 days of incubation in the Mueller Hinton Broth (MHB) supplemented with 6 mg/L of naproxen (Gren et al 2010). Zhang et al (2016) conducted a study within a subsurface flow wetland planted with Typha angustifolia, which was employed to treat an effluent contaminated with 250 μg/L of caffeine, demonstrating that T. angustifolia removed caffeine with an efficiency of 93%. Moreover, characterization of non-cultivable bacterial communities associated with the roots of T. angustifolia revealed that plant is colonized with at least 20 phyla, among which the most predominant were Firmicutes (39.0%), Actinobacteria (25.1%), and Proteobacteria (17.1%).…”

Responses of the Endophytic Bacterial Communities of Juncus acutus to Pollution With Metals, Emerging Organic Pollutants and to Bioaugmentation With Indigenous Strains

“…Only a few studies attempted to explore the bacterial communities associated with wetland plants and even fewer to describe the responses of such communities to mixed and highly polluted environments ( Su et al, 2015 ; Zhao et al, 2015 ; Zhang D. et al, 2016 ; Syranidou et al, 2017b ). Moreover, information relevant to the impacts of pollutants on the endophytic bacteria of wetland plants is scarce.…”