Purification and characterization of a novel glutamate dehydrogenase from Geotrichum candidum with higher alcohol and amino acid activity

Zhu, Jing; Lu, Kuan; Xu, Xiaoguang; Wang, Xinglong; Shi, Junling

doi:10.1186/s13568-016-0307-8

Cited by 8 publications

(6 citation statements)

References 49 publications

(52 reference statements)

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“…are yeast‐like fungi widely used in biotechnology, for example in cheese making, in the biodegradation of oil stains and decomposition of cellulose (Hyde et al, 2019). Zhu et al, (2017) showed significant degradation of higher alcohols, such as hexanol and isoamyl alcohol, at low pH by glutamate dehydrogenase produced by the species G. candidum .…”

Section: Discussionmentioning

confidence: 99%

Preservation fluids of heritage anatomical specimens — a challenge for modern science. Studies of the origin, composition and microbiological contamination of old museum collections

et al. 2023

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Anatomical museums preserve specimens of great historical value and undiscovered scientific potential. However, frequently these collections lack documentation of the techniques of preparation and the composition of preservative substances (conservation principles). This poses a huge problem for the care and preservation of these materials, more so because understanding this issue requires knowledge of fundamentals from different scientific disciplines. The aim of the research was to obtain information about the composition of substances used to preserve historic specimens, as well as to conduct a microbiological assessment of the specimens to detect possible factors causing their deterioration. Furthermore, we wanted to fill an existing gap in the literature, as there is a lack of reports on analytical methods that could be successfully applied by anatomists involved in the daily care of museum collections in human anatomy departments. The starting point was the analysis of the sources and history of the collections, on which basis the choice of research methods was made. Methods based on simple chemical reactions and specialised methods (such as gas chromatography-tandem mass spectrometry, Fourier transform infrared spectroscopy, inductively coupled plasma optical emission spectroscopy) were used in the analyses of the composition of fluids. Microbiological analyses were based on culture and isolation methods, analysis of microscopy slides and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry analysis. As a result of these analyses, some components of the preservative mixtures and their concentrations were determined. The presence of methanol, ethanol, formaldehyde and glycerol was detected, among other chemicals.The concentrations of these substances were different between the samples and their determination required the use of a variety of methods suitable for the individual components of the preservative mixture. In microbiological tests, both bacteria and fungi were isolated from swabs taken from anatomical specimens. The bacterial flora was less numerous than the fungal flora. Among the bacteria, environmental Gram-positive Bacillus cereus, Bacillus thuringiensis and a rare bacterium of the Cupriavidus genus were | 149 DOMAŃSKI et al.

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Section: Discussionmentioning

confidence: 99%

Preservation fluids of heritage anatomical specimens — a challenge for modern science. Studies of the origin, composition and microbiological contamination of old museum collections

et al. 2023

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“…G. candidum S12 was shown to produce a novel glutamate dehydrogenase, which was highly active against glutamate, hexanol, α-ketoglutarate, and isoamyl alcohol (Km values of 41.74, 4.01, 20.37, and 19.37 mM, respectively) [ 144 ]. The catalytic activity against hexanol was enhanced by the addition of ADP, K + , Fe 2+ , and Zn 2+ and reduced by EDTA, Mn 2+ , Pb 2+ , ATP, and DTT.…”

Section: Production Of Enzymes With Industrial Interestmentioning

confidence: 99%

Uncovering the Biotechnological Importance of Geotrichum candidum

Kamilari

Stanton

Reen

et al. 2023

Foods

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Fungi make a fundamental contribution to several biotechnological processes, including brewing, winemaking, and the production of enzymes, organic acids, alcohols, antibiotics, and pharmaceuticals. The present review explores the biotechnological importance of the filamentous yeast-like fungus Geotrichum candidum, a ubiquitous species known for its use as a starter in the dairy industry. To uncover G. candidum’s biotechnological role, we performed a search for related work through the scientific indexing internet services, Web of Science and Google Scholar. The following query was used: Geotrichum candidum, producing about 6500 scientific papers from 2017 to 2022. From these, approximately 150 that were associated with industrial applications of G. candidum were selected. Our analysis revealed that apart from its role as a starter in the dairy and brewing industries, this species has been administered as a probiotic nutritional supplement in fish, indicating improvements in developmental and immunological parameters. Strains of this species produce a plethora of biotechnologically important enzymes, including cellulases, β-glucanases, xylanases, lipases, proteases, and α-amylases. Moreover, strains that produce antimicrobial compounds and that are capable of bioremediation were identified. The findings of the present review demonstrate the importance of G. candidum for agrifood- and bio-industries and provide further insights into its potential future biotechnological roles.

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Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 99%

“…In the same manner as monovalent, divalent cations can be both activators and inhibitors of NADP + -glutamate dehydrogenase activity in microorganisms [16,17]. Geotrichum candidum S12 glutamate dehydrogenase is unique, as it is characterized by substrate specificity for glutamate, 2-oxoglutarate, hexanol, and isoamil alcohol (3-methyl 1-butanol) [17]. In the presence of adenosine diphosphoric acid, Fe 2+ , K + , and Zn 2+ , an increase in enzymatic activity relative to hexanol was observed, and in the presence of EDTA, Mn 2+ , and adenosine triphosphoric acid, its inhibition was observed.…”

Section: Abstract: Acinetobacter Calcoaceticus Imv B-7241 Surfactants Potassium and Sodium Cations Biological Activity Biosynthesismentioning

confidence: 99%

Synthesis and Biological Activity of Acinetobacter calcoaceticus IMV B-7241 Surfactants Depending on Monovalent Cations Content in Cultivation Medium

Пирог¹,

Lutsai²,

Shevchuk³

et al. 2021

Mikrobiol. Z.

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Microbial surfactants (biosurfactants) are multifunctional preparations due to a combination of physicochemical (reduction of surface and interfacial tension, emulsifying activity) and biological (antimicrobial and antiadhesive activity, the ability to destroy biofilms) properties. However, the disadvantage of biosurfactants synthesized as a complex of compounds is the possibility of changing the biological activity depending on the conditions of producer cultivation. Aim. To study the effect of potassium and sodium cations on the NADP+-dependent glutamate dehydrogenase activity of cell-free extract of Acinetobacter calcoaceticus ІМV B-7241 with subsequent appropriate modification of the nutrient medium composition and determination of antimicrobial and anti-adhesive activity of surfactant synthesized. Methods. A. calcoaceticus ІМV B-7241 strain was grown in media containing 2% of sunflower oil waste as a carbon source, as well as various concentrations of potassium and sodium chloride (basal – 1.0 g/l NaCl, medium #1 that did not contains NaCl, medium #2 in which the concentration of NaCl was 2.0 g/l, medium #3 in which the concentration of NaCl and KCl was 1.0 g/l each). The surfactants were extracted from the supernatant liquid culture with a modified Folch mixture. Antiadhesive activity and the degree of biofilms degradation were determined by spectrophotometric method, antimicrobial activity − by the indicator of the minimum inhibitory concentration (MIC). Activity of enzymes of surface-active aminolipids biosynthesis (NADP+-dependent glutamate dehydrogenase) and glycolipids (phosphoenolpyruvate (PEP) carboxylase, PEP-synthetase, PEP-carboxykinase, trehalose phosphate synthase) were analyzed in cell-free extracts obtained after the destruction of cells by ultrasound. Results. It was found that potassium and sodium cations in concentrations of 50 and 100 mM are inhibitors of NADP+-dependent glutamate dehydrogenase, and in lower concentrations (5–20 mM) – activators of this enzyme, as well as PEP-carboxykinase and PEP-synthetase. The increase in the biosurfactant concentration to 6.1−7.7 g/l during cultivation of A. calcoaceticus ІМV B-7241 in medium #1 and #3 was due to the predominant synthesis of glycolipids under such conditions, which was evidenced by the increase in 1.8−6.5 times in the activity of PEP-carboxylase, PEP-carboxykinase, PEP-synthetase and trehalose phosphate synthetase compared to the indicators on the basal medium. The concentration of surfactants synthesized in the basal medium was 3.6 g/l, but such surfactants were characterized by the highest antimicrobial and anti-adhesive activity. Their MIC against the test-cultures of studied bacteria (Pseudomonas sp. MI-2, Bacillus subtilis BT-2, Escherichia coli IEM-1, Staphylococcus aureus BMS-1, Enterobacter cloaceae C-8) and fungi (Candida albicans D-6, Rhizopus nigricans P1, Aspergillus niger P-3, Fusarium culmorum T-7) were 0.88−56 μg/ml and were by 2−3 orders of magnitude lower compared to established for surfactants synthesized in modified media #1–3. In the case of treatment of abiotic materials with surfactant solutions obtained on the basal medium, the adhesion of bacteria and fungi was on average 10–20% lower than after surface treatment by the surfactant synthesized in modified media. In the presence of 148−296 μg/ml of surfactants obtained in the basal medium, destruction of S. aureus BMS-1 and B. subtilis BT-2 biofilms was 45−66%, and C. albicans D-6 yeast – 39−44%. Under the action of similar concentrations of surfactants synthesized in modified media, the destruction of bacterial and yeast biofilms was lower: 6-52 and 20–46%, respectively. Conclusions. The obtained results are consistent with the data of our previous studies on the possibility of regulating the antimicrobial and antiadhesive activity of surfactants in the process of producer cultivation by changing the content of cations in the medium, which are inhibitors/activators of enzymes responsible for the synthesis of components of the surfactants complex, which have certain biological properties.

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Purification and characterization of a novel glutamate dehydrogenase from Geotrichum candidum with higher alcohol and amino acid activity

Cited by 8 publications

References 49 publications

Preservation fluids of heritage anatomical specimens — a challenge for modern science. Studies of the origin, composition and microbiological contamination of old museum collections

Preservation fluids of heritage anatomical specimens — a challenge for modern science. Studies of the origin, composition and microbiological contamination of old museum collections

Uncovering the Biotechnological Importance of Geotrichum candidum

Synthesis and Biological Activity of Acinetobacter calcoaceticus IMV B-7241 Surfactants Depending on Monovalent Cations Content in Cultivation Medium

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