Purification and characterization of a novel protein with activity against non-small-cell lung cancer in vitro and in vivo from the edible mushroom Boletus edulis

Zhang, Yang; Zhou, Rong; Liu, Fang; Ng, Tzi Bun

doi:10.1016/j.ijbiomac.2021.01.149

Cited by 23 publications

(12 citation statements)

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“…A Boletus edulis anti-tumor protein (BEAP) with a MW of 16.7 KD displayed strong anti-cancer activity on A549 (human non-small-cell lung cancer) cells both in vitro and in vivo. BEAP cytotoxicity was aided by the activation of apoptosis and the arrest of A549 cells in the G1 phase of the cell cycle [ 50 ]. Likewise, the protein extract, PS60 from P. tuber-regium sclerotium was the most effective protein extract against the breast cancer cell line MDA-MB-23.…”

Section: Therapeutic Properties Of Edible Mushroom Proteinsmentioning

confidence: 99%

“…Another important feature of bioactive proteins is the ability to be used as pharmaceutical agents. Mushroom proteins and peptides have antihypertensive, immunomodulatory, antifungal, antibiotic, and antibacterial activities, and anticancer, antiviral, antioxidant, and ACE inhibitory effects [ 50 , 52 , 78 •, 86 ]. Considering the different biological activities in Table 1 (searched articles within 2020–2022) it emerged that about 25% of bioactive proteins possessed ACE inhibitory activity, mainly peptides (single and mixture) and only one protein hydrolysate.…”

Section: Mushroom Proteins Are Important Pharmaceutical Agentsmentioning

confidence: 99%

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Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Ayimbila

Keawsompong

2023

Curr Nutr Rep

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Purpose of Review Global concerns about population growth, economic, and nutritional transitions and health have led to the search for a low-cost protein alternative to animal origins. This review provides an overview of the viability of exploring mushroom protein as a future protein alternative considering the nutritional value, quality, digestibility, and biological benefits. Recent Findings Plant proteins are commonly used as alternatives to animal proteins, but the majority of them are low in quality due to a lack of one or more essential amino acids. Edible mushroom proteins usually have a complete essential amino acid profile, meet dietary requirements, and provide economic advantages over animal and plant sources. Mushroom proteins may provide health advantages by eliciting antioxidant, antitumor, angiotensin-converting enzyme (ACE), inhibitory and antimicrobial properties over animal proteins. Protein concentrates, hydrolysates, and peptides from mushrooms are being used to improve human health. Also, edible mushrooms can be used to fortify traditional food to increase protein value and functional qualities. These characteristics highlight mushroom proteins as inexpensive, high-quality proteins that can be used as a meat alternative, as pharmaceuticals, and as treatments to alleviate malnutrition. Summary Edible mushroom proteins are high in quality, low in cost, widely available, and meet environmental and social requirements, making them suitable as sustainable alternative proteins.

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Section: Therapeutic Properties Of Edible Mushroom Proteinsmentioning

confidence: 99%

Section: Mushroom Proteins Are Important Pharmaceutical Agentsmentioning

confidence: 99%

Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Ayimbila

Keawsompong

2023

Curr Nutr Rep

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“…For instance, according to previous studies, many edible and medicinal fungi contain a variety of bioactive components, including polysaccharides, phenols, purine, and immunoregulatory proteins, thus exerting diverse pharmacological activities [11]. It is worth mentioning that the anti-tumor effects of edible and medicinal fungi have been confirmed in both in vitro and in vivo models of lung cancer [12], liver cancer [13], and breast cancer [14]. Moreover, fungal polysaccharides, the major bioactive ingredients in many types of edible and medicinal fungi, can display anti-tumor effects by improving the immunity of the body [15][16][17].…”

Section: The Role Of Edible and Medicinal Fungi In Traditional Usesmentioning

confidence: 99%

Current Advancements in Antitumor Properties and Mechanisms of Medicinal Components in Edible Mushrooms

Shen

Jiao

et al. 2022

Nutrients

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Edible and medicinal fungi, a group of eukaryotic organisms with numerous varieties, including Coriolus versicolor, Ganoderma lucidum, Cordyceps sinensis, Pleurotus ostreatus, and Grifola frondosa, have been demonstrated to possess a board range of pharmaceutical properties, including anti-virus, anti-inflammation, and neuroprotection. Moreover, edible and medicinal fungi have been traditionally consumed as food to provide multiple nutrients and as drugs owing to having the activities of invigorating blood circulation, reinforcing the healthy qi, clearing away heat, and eliminating stasis for thousands of years in China. Malignant tumors, well-known as the second leading cause of death globally, accounted for nearly 10 million deaths in 2020. Thus, in-depth exploration of strategies to prevent and treat cancer is extremely urgent. A variety of studies have reported that the main bioactive components of edible and medicinal fungi, mainly polysaccharides and triterpenoids, exhibit diverse anticancer activities via multiple mechanisms, including inhibition of cell proliferation and metastasis, induction of apoptosis and autophagy, reversing multidrug resistance, and regulation of immune responses, thus suggesting their substantial potential in the prevention and treatment of cancer. Our review summarizes the research progress on the anticancer properties of edible and medicinal fungi and the underlying molecular mechanism, which may offer a better understanding of this field. Additionally, few studies have reported the safety and efficacy of extracts from edible and medicinal fungi, which may limit their clinical application. In summary, there is a need to continue to explore the use of those extracts and to further validate their safety and efficacy.

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“…Furthermore, fresh mushrooms possess a variety of compounds such as polysaccharides, peptides, ergosterol, polyphenolics, and lectins, that contribute to anticancer, antihypertensive, hepatoprotective, and antioxidant activities; in addition, these compounds are beneficial to human microbiota modulation and exhibit anti‐obesity, antiproliferative and antiviral activities (El‐Deeb et al, 2019; El‐Maradny, El‐Fakharany, Abu‐Serie, Hashish, & Selim, 2021; Khatun, Sato, & Konishi, 2020). For the past few years, the sales of edible mushrooms have been continually increasing, and the per capita sales have even reached 20–24 kg in China (Zhang, Zhou, Liu, & Ng, 2021). Food and Agriculture Organization (FAO) highly recommended Stropharia rugosoannulata to developing countries because of its value, and the outputs and consumption of S. rugosoannulata have been augmenting notably in the Chinese market (Song et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…continually increasing, and the per capita sales have even reached 20-24 kg in China (Zhang, Zhou, Liu, & Ng, 2021). Food and Agriculture Organization (FAO) highly recommended Stropharia rugosoannulata to developing countries because of its value, and the outputs and consumption of S. rugosoannulata have been augmenting notably in the Chinese market (Song et al, 2009).…”

mentioning

confidence: 99%

Effects of nanocomposite packaging on postharvest quality of mushrooms (Stropharia rugosoannulata) from the perspective of water migration and microstructure changes

Mao-bin

Liu

et al. 2023

Journal of Food Safety

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Stropharia rugosoannulata, a high‐value mushroom, exhibits high nutritional value and flavor characteristics; however, the mushroom is susceptible to spoilage, water loss and texture deterioration, which renders its safety and edibility a challenge. To improve the shelf life of fresh mushrooms during storage, a novel nanocomposite packaging (NP) was applied for the first time to maintain the freshness of S. rugosoannulata. The results showed that compared to other groups, NP effectively delayed the deterioration of sensory quality, postponed the decrease in firmness, and inhibited the degradation of soluble protein (caps 54.58 mg g−1 and stipes 31.45 mg g−1) and crude polysaccharide (caps 5.06 mg g−1 and stipes 3.53 mg g−1). Moreover, NP significantly reduced weight loss (0.41%) and water content loss (caps 90.93% and stipes 89.80%), as confirmed by low‐field nuclear magnetic resonance and magnetic resonance imaging; thus, water loss was inhibited. Additionally, the results of scanning electron microscopy and transmission electron microscopy indicated that NP helped preserve intact cell structure and retard organelle disintegration, helping prevent water from escaping to some extent. The overall research indicated that the application and performance of the novel NP, which is convenience, suitable for scale application and improves postharvest quality, is a promising approach for extending the shelf life of fresh S. rugosoannulata.

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Purification and characterization of a novel protein with activity against non-small-cell lung cancer in vitro and in vivo from the edible mushroom Boletus edulis

Cited by 23 publications

References 61 publications

Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Nutritional Quality and Biological Application of Mushroom Protein as a Novel Protein Alternative

Current Advancements in Antitumor Properties and Mechanisms of Medicinal Components in Edible Mushrooms

Effects of nanocomposite packaging on postharvest quality of mushrooms (Stropharia rugosoannulata) from the perspective of water migration and microstructure changes

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