Purification and Characterization of a Potato Tuber Acid Phosphatase Having Significant Phosphotyrosine Phosphatase Activity

Gellatly, Kevin S.; Moorhead, Greg B. G.; Duff, Stephen M. G.; Lefebvre, Daniel D.; Plaxton, William C.

doi:10.1104/pp.106.1.223

Cited by 73 publications

(60 citation statements)

References 21 publications

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“…From a mechanistic perspective, the enzyme could either play an antioxidant role by scavenging free radicals, or take part in the production of reactive oxygen species for membrane peroxidation during tissue senescence (del Pozo et al 1999;Oddie et al 2000). Another specialized category of plant APase consists of those that display a clear, but non-absolute, speci®city for substrates such as 3-phosphoglycerate (Randall and Tolbert 1971;Villareal and Juliano 1977), PEP (Du et al 1989), phytate (Gibson and Ullah 1988), and phosphotyrosine (Gellatly et al 1994;Roknabadi et al 1999). These APases are hypothesized to have distinct metabolic functions.…”

Section: Introductionmentioning

confidence: 98%

Purification and characterization of banana fruit acid phosphatase

Turner

Plaxton

2001

Planta

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An acid phosphatase (APase, EC 3.1.3.2) from ripened banana (Musa cavendishii L. cv. Cavendish) fruit has been purified 1,876-fold to electrophoretic homogeneity and a final p-nitrophenylphosphate (pNPP)-hydrolyzing specific activity of 745 micromol Pi produced (mg protein)(-1) min(-1). Non-denaturing PAGE of the final preparation resolved a single protein-staining band that co-migrated with APase activity. SDS-PAGE and analytical gel filtration demonstrated that the purified enzyme exists as a 40-kDa monomer. That the enzyme is glycosylated was indicated by its tight absorption to Concanavalin A-Sepharose. Banana APase was relatively heat stable, displayed a symmetrical pH/activity profile with maximal activity at pH 5.8, and was activated 180% and 150% by 5 mM Mn2+ and Mg2+, respectively. The enzyme exhibited a broad substrate selectivity, with maximal specificity constants (Vmax/Km) obtained with pNPP, phosphoenolpyruvate, phenyl phosphate, and O-phospho-L-tyrosine. Potent inhibition by Pi, molybdate, vanadate, arsenate, and Zn2+ was observed. Putative metabolic functions of the APase are discussed in relation to maintaining significant Pi mobility during banana fruit ripening.

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Section: Introductionmentioning

confidence: 98%

Purification and characterization of banana fruit acid phosphatase

Turner

Plaxton

2001

Planta

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“…The biochemical hydrolysis of organic phosphate esters in soils is mainly catalysed by phosphomonoesterases and phosphodiesterases, which release orthophosphate anions, the preferentially assimilated P form by plants and soil microorganisms. The phosphomonoesterase activity is the main mechanism for P acquisition by plants, although more complex mechanisms of P solubilization by specific plants and phosphatases generally catalyse P through orthophosphate esters and anhydrides (Gellatly et al 1994;Nannipieri et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Changes in phosphorus content, phosphatase activity and some physicochemical and microbiological parameters of soil within the range of impact of illegal dumping sites in Bydgoszcz (Poland)

2016

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The present research aimed to assess the influence of illegal dumping on changes in the content of phosphorus and its relationship with selected chemical properties. Soil was sampled from two horizons: 0-20 and 20-40 cm. Samples from points W1, W2 and W3 (waste 1, 2 and 3) were collected directly from under illegally deposited landfill sites that differed in the morphological composition. At a distance of 30 m from landfill W3 there were agricultural lands where maize was cultivated (arable soil, AS). The control point (C) was located far from the waste landfill sites determined and beyond the range of their effect. In soil the following were determined: total organic carbon, phosphorus (total, organic, mineral, available, active), the microbiological activity, the content of heavy metals and the activity of alkaline and acid phosphatase. The study concluded that the composition of treatments W1 and W2 had a more negative impact on the soils than landfill site W3. The results show that the changes depended on the type of the waste deposited. In AS and landfills W3 there was observed a high P availability and count of bacteria. The results have shown that an addition of organic residues to soil has a variety of effects on microbial and phosphatase activities. The lowest resistance (RS) for alkaline and acid phosphatases was found in soil from the area of dumping sites W1 and W2; the highest RS was determined in AS. Statistical analyses of simple correlations showed clearly that microbiological populations were also particularly implicated in the activity of phosphomonoesterases.

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“…With respect to the present study, B. nigra cells are known to produce elevated amounts of phenolic compounds during Pi deprivation [14]. In addition to a possible role in modifying glycosylation of these phenolic compounds, a Pi-starvationinducible fl-glucosidase might also be involved in the deglycosylation and, hence, regulation of phosphatases and other enzymes during this stress [2,15].…”

Section: Discussionmentioning

confidence: 99%

Isolation of cDNA clones of genes with altered expression levels in phosphate-starved Brassica nigra suspension cells

Malboobi

Lefebvre

1995

Plant Mol Biol

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Differential gene expression at the transcriptional level was examined as an initial step in the investigation of the P(i) starvation response of Brassica nigra suspension cells. Total RNA was extracted from 7-day old cells grown in media containing either no P(i), 1.25 mM or 10 mM Pi. In vitro translation was carried out using their respective poly(A)+ RNA isolates and the resultant polypeptides were separated on a high-resolution SDS-PAGE gel. Scanning densitometry identified four polypeptides (ca. 31.7, 32.3, 52.5 and 64.8 kDa) present only in the P(i)-starved samples. Screening by differential hybridization was performed on a cDNA library constructed from mRNA isolated from P(i)-starved cells. Probes prepared from mRNA from P(i)-deficient and P(i)-sufficient cells identified a number of clones representing mRNA species that were preferentially transcribed under P(i) deficiency. These phosphate starvation-responsive (psr) clones were placed into eleven groups as determined by cross-hybridization. Northern blots showed that the corresponding genes are inducible in both mild and severe P(i) starvation conditions. Preliminary sequencing identified one of the clones as being homologous to beta-glucosidases from several plant species. The possible role of beta-glucosidase during Pi starvation and the identities of the other psr genes are discussed.

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Purification and Characterization of a Potato Tuber Acid Phosphatase Having Significant Phosphotyrosine Phosphatase Activity

Cited by 73 publications

References 21 publications

Purification and characterization of banana fruit acid phosphatase

Purification and characterization of banana fruit acid phosphatase

Changes in phosphorus content, phosphatase activity and some physicochemical and microbiological parameters of soil within the range of impact of illegal dumping sites in Bydgoszcz (Poland)

Isolation of cDNA clones of genes with altered expression levels in phosphate-starved Brassica nigra suspension cells

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