Isolation of cDNA clones of genes with altered expression levels in phosphate-starved Brassica nigra suspension cells

Malboobi, M. A.; Lefebvre, Daniel D.

doi:10.1007/bf00042071

Cited by 23 publications

(9 citation statements)

References 52 publications

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“…Several experiments have studied the role of high-affinity P-transporters in plant P uptake. Malboobi and Lefebvre (1995) and Leggewie et al (1997) both reported higher transcript levels for the high-affinity P-transporter under conditions of P deprivation. Muchhal and Raghothama (1999) reported that the expression of the P-transporter, LePT1, in tomato was altered by P availability of the growth medium.…”

Section: P-transporter Steady-state Transcript Levelsmentioning

confidence: 93%

Phosphate nutrition effects on growth, phosphate transporter transcript levels and physiology of alfalfa cells

Qamar

Sors

Cunningham

et al. 2005

Plant Cell Tiss Organ Cult

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Phosphorus deficiency reduces forage yield and stand persistence of alfalfa (Medicago sativa L.). Our objectives were to isolate and characterize a high-affinity phosphate-transporter (P-transporter) from alfalfa roots (Medicago sativa L.); determine how phosphorus (P) nutrition impacts P-uptake, growth, and carbohydrate and protein metabolism of alfalfa cells; and learn how expression of the P-transporter is influenced by P nutrition. An 1087-base pair (bp) sequence was isolated using RT-PCR that possessed high nucleotide and amino acid sequence similarity to high-affinity P-transporters. Cultured cells were sampled at 3-day intervals for 9 days while growing in media containing P concentrations ranging from 0 to 10 mM. Media P concentrations declined rapidly in all P treatments by day 6. Low media P concentrations (0, 0.1 and 0.5 mM) reduced cell growth rates compared to higher media P levels (2.5, 5 and 10 mM). Suspension cell cultures supplied 0.5, 2.5, 5, and 10 mM P had lower starch concentrations by day 3 compared to cells cultured in media containing 0 and 0.1 mM P. Steady-state transcript levels for the high-affinity P-transporter were high in P-deprived cells, but declined within 1 day when cells were provided 10 mM P.Abbreviations: 2,4-D D -2,4-dichlorophenoxyacetic acid; MS -Murashige and Skoog; P -phosphorus; P-transporter -phosphate-transporter

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Section: P-transporter Steady-state Transcript Levelsmentioning

confidence: 93%

Phosphate nutrition effects on growth, phosphate transporter transcript levels and physiology of alfalfa cells

Qamar

Sors

Cunningham

et al. 2005

Plant Cell Tiss Organ Cult

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“…4A). Previous studies showed that the gene coding beta-glucosidase was induced by P starvation in Brassica nigra [48].…”

Section: Oaamentioning

confidence: 99%

Proteomic and comparative genomic analysis reveals adaptability of Brassica napus to phosphorus-deficient stress

Chen

Ding

Wang

et al. 2015

Journal of Proteomics

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“…Differential Transcriptional Responses of Arabidopsis PAPs to Phosphate Deprivation-Because suspension cultures of different plant species had been employed in past studies on the regulation of many types of genes by phosphate deprivation treatment (31,(45)(46)(47)(48)(49)(50)(51)(52)(53), the transcriptional responses of AtPAP7-AtPAP13 to phosphate deprivation treatment were investigated using Arabidopsis suspension cells. Total RNA was prepared from both control and phosphate-deprived cell samples.…”

Section: Phylogenetic and Structural Relationships Of Arabidopsis Papmentioning

confidence: 99%

Purple Acid Phosphatases of Arabidopsis thaliana

Li¹,

Zhu²,

Liu³

et al. 2002

Journal of Biological Chemistry

251

130

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Purple acid phosphatases (PAPs) are members of the metallo-phosphoesterase family. They are characterized by the presence of seven conserved amino acid residues involved in coordinating the dimetal nuclear center in their reactive site. We compared the 29 PAPs predicted for Arabidopsis thaliana in their varieties of potential metal-ligating residues. Although 24 members possessed sets of metal-ligating residues typical of known PAPs, 1 member lacked four of the seven residues. For the remaining four members, potential metal-ligating residues were generally more similar to those in metal-dependent exonucleases and related proteins. Evidence was obtained for the expression of the majority of the 29 PAPs. To facilitate future investigations, a scheme for naming Arabidopsis PAPs and a system for classifying the 29 PAPs are proposed. The cDNA sequences and the responses to phosphate deprivation of seven Arabidopsis PAPs (AtPAP7-AtPAP13) were characterized. For some AtPAPs analyzed, there were fully processed transcripts as well as splice variants. The splice variants of AtPAP10 were found to associate with polyribosomes and may be translated into a NH 2 -terminal truncated protein. Phylogenetic investigations showed that AtPAPs 7 and 8, together with similar enzymes from other plant species, formed the low molecular weight plant PAP group. Members of this group were more closely related to PAPs from mammalian cells. AtPAPs 9 -13, together with kidney bean PAP, formed the high molecular weight PAP group. In phosphate deprivation experiments, gene transcription of AtPAP11 and AtPAP12 was induced and increased, respectively, whereas that of the remaining five AtPAPs was not affected by phosphate deprivation. The present work demonstrates that structure variation and expression regulation of plant PAPs are more complex than previously described and provides a framework for comprehensive molecular genetic and biochemical studies of all Arabidopsis PAPs in the future.

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Isolation of cDNA clones of genes with altered expression levels in phosphate-starved Brassica nigra suspension cells

Cited by 23 publications

References 52 publications

Phosphate nutrition effects on growth, phosphate transporter transcript levels and physiology of alfalfa cells

Phosphate nutrition effects on growth, phosphate transporter transcript levels and physiology of alfalfa cells

Proteomic and comparative genomic analysis reveals adaptability of Brassica napus to phosphorus-deficient stress

Purple Acid Phosphatases of Arabidopsis thaliana

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