Purification and characterization of a proteolytic enzyme from Candida albicans

The proteolytic potential of Candida albicans in human saliva supplemented with glucose

“…The acidic proteases produced by C. albicans may contribute to the pathogenesis of this disease which is particularly prevalent in elderly denture wearers (Budtz-Jorgensen, 1974a;198 1;Birkeland, 1975, 1976;Zgraggen and Graf, 1975). Remold, Fasold and Staib (1968) demonstrated that protease-producing strains of C. albicans caused more extensive infection in mice than protease-deficient strains, although Budtz-Jorgensen (1 974a) could find no direct relationship between protease production and infectivity.…”

An Examination of the Production of Hydrolytic Enzymes and Toxins by Pathogenic Strains of Candida albicans

“…In some experiments the medium of De Palma (1966) was used which gives blastospores at 37" and the mycelial form at the same temperature when sodium bicarbonate is added to give pH 7.5. For preparation of the extracellular peptidase the medium of Remold et al (1968) was used.…”

“…The last was isolated from culture filtrates (Remold, Fasold & Staib, 1968) grown on a serum albumin containing medium at pH 4.0.…”

“…Its formation under the conditions described by Staib has been confirmed in 36/37 strains tested. The one strain (c-a-I) which did not produce it was isolated from a child with widespread candidiasis of several years duration and was highly lethal to mice; Staib (1965) describes a strain of similar origin, but this and other strains which did not produce the enzyme were found by Remold et al (1968) not to cause widespread infection in mice. Several groups of workers have reported the production of toxin by single strains of Candida dbicans, using a variety of growth conditions.…”

The Cellular Location of Proteases in Candida albicans

“…Pepstatin-sensitive proteinase activity in these preparations probably represents proteinase ycaA (Remold et al, 1968). Vacuole preparations were enriched in a-Dmannosidase (marker enzyme for the vacuolar membrane; Schekman, 1982), alkaline phosphatase (marker enzyme for the soluble contents of the yeast vacuole) and proteinase ( Table 1).…”