Purification and biological activity of the thrombin-like substance isolated from Bothrops insularis venom

Braga, Marcus Davis Machado; Martins, Alice Maria Costa; Menezes, Dalgimar Beserra de; Barbosa, Paula; Evangelista, Janaína Serra Azul Monteiro; Toyama, Marcos Hikari; Toyama, Daniela O.; Fonteles, M. C.; Monteiro, Helena Serra Azul

doi:10.1016/j.toxicon.2006.10.005

Cited by 9 publications

(6 citation statements)

References 36 publications

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“…Braga et al (2007) demonstrated the effects of Bothrops insularis venom on isolated kidneys. Recently, Braga et al (2008) showed renal effects of phospholipase A 2 isolated from Bothrops insularis venom.…”

Section: Introductionmentioning

confidence: 99%

Renal and cardiovascular effects of Bothrops marajoensis venom and phospholipase A2

Evangelista

Martins

Nascimento

et al. 2010

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Section: Introductionmentioning

confidence: 99%

Renal and cardiovascular effects of Bothrops marajoensis venom and phospholipase A2

Evangelista

Martins

Nascimento

et al. 2010

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“…Among several enzymes in Bothrops venom, L-amino acid oxidase, phospholipase A 2 , metalloprotease and sphingomyelinase are believed to contribute to renal toxicity [ 34 ]. In addition, perfusion of isolated rat kidneys with LAAO [ 35 ], C type lectins [ 36 , 37 ], phospholipase A2 (PLA2) myotoxins [ 38 , 39 ] and thrombin-like enzyme [ 40 ] from Bothrops venoms caused renal function disturbances.…”

Section: Discussionmentioning

confidence: 99%

L-Aminoacid Oxidase from Bothrops leucurus Venom Induces Nephrotoxicity via Apoptosis and Necrosis

et al. 2015

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Acute renal failure is a common complication caused by Bothrops viper envenomation. In this study, the nefrotoxicity of a main component of B. leucurus venom called L-aminoacid oxidase (LAAO-Bl) was evaluated by using tubular epithelial cell lines MDCK and HK-2 and perfused kidney from rats. LAAO-Bl exhibited cytotoxicity, inducing apoptosis and necrosis in MDCK and HK-2 cell lines in a concentration-dependent manner. MDCK apoptosis induction was accompanied by Ca2+ release from the endoplasmic reticulum, reactive oxygen species (ROS) generation and mitochondrial dysfunction with enhanced expression of Bax protein levels. LAAO-Bl induced caspase-3 and caspase-7 activation in both cell lines. LAAO-Bl (10 μg/mL) exerts significant effects on the isolated kidney perfusion increasing perfusion pressure and urinary flow and decreasing the glomerular filtration rate and sodium, potassium and chloride tubular transport. Taken together our results suggest that LAAO-Bl is responsible for the nephrotoxicity observed in the envenomation by snakebites. Moreover, the cytotoxic of LAAO-Bl to renal epithelial cells might be responsible, at least in part, for the nephrotoxicity observed in isolated kidney.

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“…Some purified Bothrops toxins are able to reproduce the renal effects obtained with whole venom. Studies on the isolated perfused rat kidney have shown that L-amino acid oxidase (Braga et al, 2008), C-type lectins (Braga et al, 2006), phospholipase A2 myotoxins (Barbosa et al, 2005;Evangelista et al, 2010) and thrombin-like enzyme (Braga et al, 2007) from Bothrops venoms can alter renal function. The isolated perfused kidney technique also confirmed the direct acute tubular nephrotoxicity of Bothrops venoms and showed that platelet activating factor might play a role in some renal functional disturbances such as the decreased in glomerular filtration rate (Monteiro and Fonteles, 1999).…”

Section: Bothrops Venommentioning

confidence: 99%

Acute Kidney Injury Induced by Snake and Arthropod Venoms

Berger¹,

Vieira²,

Guimarães³

2012

Renal Failure - The Facts

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Purification and biological activity of the thrombin-like substance isolated from Bothrops insularis venom

Cited by 9 publications

References 36 publications

Renal and cardiovascular effects of Bothrops marajoensis venom and phospholipase A2

Renal and cardiovascular effects of Bothrops marajoensis venom and phospholipase A2

L-Aminoacid Oxidase from Bothrops leucurus Venom Induces Nephrotoxicity via Apoptosis and Necrosis

Acute Kidney Injury Induced by Snake and Arthropod Venoms

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