Purification and Biochemical Characterization of Digestive Lipase in Whiteleg Shrimp

Abstract

Penaeus vannamei lipase was purified from midgut gland of whiteleg shrimp. Pure lipase (E.C. 3.1.

Marine invertebrate lipases: Comparative and functional genomic analysis

“…Few characterizations of marine invertebrate lipases have been performed, among them a lipase from the yesso scallop P. yessoensis (Kyoung et al, 2008) and the shrimp Penaeus vannamei (Rivera-Perez et al, 2011a,b). Both possess a β9 loop of 21 residues and a lid of 22 residues, and its catalytic activity against TAGs has been proved, showing a preferences for long-chain triglycerides (Rivera-Perez et al, 2011a). Other studies using crude extracts from digestive glands have shown the same tendency to hydrolyze long-chain TAGs, but having different regio-specificity (Del Monte et al, 2002;Forrellat-Barrios et al, 2004).…”

“…In contrast to mammals and insects, marine invertebrates are able to hydrolyze long-chain triglycerides (Rivera-Perez et al, 2011a;Forrellat-Barrios et al, 2004;Del Monte et al, 2002) which are associated with hydrophobicity of the lid. The lipases described so far function at pH 8.0 (Slim et al, 2007;Pasquevich et al, 2011;Rivera-Perez et al, 2011a), the same pH as human pancreatic lipase (Carriere et al, 2000). Another important characteristic of marine lipases is that they do not require colipase, a small protein cofactor, to display their activity (RiveraPerez et al, 2011a), as has been described in mammals (Carriere et al, 2000).…”

“…Only one intracellular lipase has been isolated and characterized in crustaceans (Rivera-Perez et al, 2011b). In contrast to mammals and insects, marine invertebrates are able to hydrolyze long-chain triglycerides (Rivera-Perez et al, 2011a;Forrellat-Barrios et al, 2004;Del Monte et al, 2002) which are associated with hydrophobicity of the lid. The lipases described so far function at pH 8.0 (Slim et al, 2007;Pasquevich et al, 2011;Rivera-Perez et al, 2011a), the same pH as human pancreatic lipase (Carriere et al, 2000).…”

“…Most previous studies on digestive lipases used crude enzyme preparations (Hervant et al, 1999;Perera et al, 2008) and only a few digestive lipases have been isolated (Slim et al, 2007;Rivera-Perez et al, 2011a,b). These enzymes are tissue specific in the digestive gland (Johnston et al, 2004;Johnston and Joel, 2005;Molschaniwskyj and Johnston, 2006;Rivera-Perez et al, 2011a), and they have been described as regio-specific and non-specific lipases. Only one intracellular lipase has been isolated and characterized in crustaceans (Rivera-Perez et al, 2011b).…”

Complementary Proteomic and Biochemical Analysis of Peptidases in Lobster Gastric Juice Uncovers the Functional Role of Individual Enzymes in Food Digestion

“…In decapod crustaceans, the digestive enzymes are secreted from the midgut gland (or hepatopancreas) and consist mainly of peptidases, glycosidases, lipases and nucleases. Some of these digestive enzymes have been biochemically characterized (Le Chevalier and Wormhoudt 1998;Nilsen et al 2010;Rivera-Perez et al 2011;Bibo-Verdugo et al 2015).…”