Marine invertebrate lipases: Comparative and functional genomic analysis

Rivera-Pérez, Crisalejandra

doi:10.1016/j.cbd.2015.06.001

Cited by 6 publications

(4 citation statements)

References 108 publications

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“…Studies on the lipid metabolism of the whiteleg shrimp Penaeus vannamei (Rivera-Pérez and García-Carreño 2011) found optimal enzyme activity when substrates of a similar nature to their feed source were used, compared to the synthetic substrate. Hydrolysis of neutral lipids is an important aspect of organismal development, and many organisms possess lipase genes defined as functionally hydrolysing triacylglycerol substrates (Rivera-Perez 2015). A complex suite of such enzymes probably works in tandem for digestive purposes.…”

Section: Resultsmentioning

confidence: 99%

Recombinant expression and characterisation of a lipase from the Antarctic zooplanktonSalpa thompsoni

Rayani

Cotton

Roberts

et al. 2022

Preprint

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Cold marine environments are abundant on earth and represent a rich resource for low-temperature enzymes. Here we apply in silico bioprospecting methods followed by in vitro expression and biochemical analyses to characterise a novel low-temperature lipase from the Antarctic tunicate Salpa thompsoni. A 586 amino acid pancreatic lipase-like gene was identified from S. thompsoni transcriptomic data, expressed as a hexahistidine fusion protein in Escherichia coli at 10 °C and purified by affinity chromatography. Hydrolysis of the synthetic substrate p-nitrophenyl butyrate (PNPB) showed that this recombinant protein has optimal activity at 20 °C and pH 7, and specific activity of 3.16 U/mg under this condition. Over 60% of enzyme activity was maintained between 15 to 25 °C, with a sharp decrease outside this range. These results are indicative of cold-active psychrophilic enzyme activity. A meta-analysis of lipase activities towards PNPB showed that the novel S. thompsoni lipase displays a higher activity at lower temperatures relative to previously characterised enzymes. The work demonstrates a methodology for the conversion of transcriptomic to in vitro expression data for the discovery of new cold-active biocatalysts from marine organisms.

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Section: Resultsmentioning

confidence: 99%

Recombinant expression and characterisation of a lipase from the Antarctic zooplanktonSalpa thompsoni

Rayani

Cotton

Roberts

et al. 2022

Preprint

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“…Lipases are a special type of serine hydrolase, which are widely found in vertebrates and invertebrates and play a crucial role in fat metabolism [14]. According to sequence relationships within the α/β hydrolase fold superfamily of proteins, lipases are divided into six families: neutral (Pfam: PF00151), acid (Pfam: PF04083), lipase 2 (Pfam: PF01674), lipase 3 (Pfam: PF01764), GDSL (Pfam: PF00657) and hormone sensitive lipases (HSL) [120, 121]. The genes encoding lipases were identified in the genomes of many insects (S6 Table) [121, 122].…”

Section: Resultsmentioning

confidence: 99%

De novo transcriptome analysis and identification of genes associated with immunity, detoxification and energy metabolism from the fat body of the tephritid gall fly, Procecidochares utilis

Gao

Lan

et al. 2019

PLoS ONE

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The fat body, a multifunctional organ analogous to the liver and fat tissue of vertebrates, plays an important role in insect life cycles. The fat body is involved in protein storage, energy metabolism, elimination of xenobiotics, and production of immunity regulator-like proteins. However, the molecular mechanism of the fat body’s physiological functions in the tephritid stem gall-forming fly, Procecidochares utilis, are still unknown. In this study, we performed transcriptome analysis of the fat body of P. utilis using Illumina sequencing technology. In total, 3.71 G of clean reads were obtained and assembled into 30,559 unigenes, with an average length of 539 bp. Among those unigenes, 21,439 (70.16%) were annotated based on sequence similarity to proteins in NCBI’s non-redundant protein sequence database (Nr). Sequences were also compared to NCBI’s non-redundant nucleotide sequence database (Nt), a manually curated and reviewed protein sequence database (SwissProt), and KEGG and gene ontology annotations were applied to better understand the functions of these unigenes. A comparative analysis was performed to identify unigenes related to detoxification, immunity and energy metabolism. Many unigenes involved in detoxification were identified, including 50 unigenes of putative cytochrome P450s (P450s), 18 of glutathione S-transferases (GSTs), 35 of carboxylesterases (CarEs) and 26 of ATP-binding cassette (ABC) transporters. Many unigenes related to immunity were identified, including 17 putative serpin genes, five peptidoglycan recognition proteins (PGRPs) and four lysozyme genes. In addition, unigenes potentially involved in energy metabolism, including 18 lipase genes, five fatty acid synthase (FAS) genes and six elongases of very long chain fatty acid (ELOVL) genes, were identified. This transcriptome improves our genetic understanding of P. utilis and the identification of a numerous transcripts in the fat body of P. utilis offer a series of valuable molecular resources for future studies on the functions of these genes.

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“…It was found that tandem gene duplicates could lead to the overactivity of some genes in Alcohol dehydrogenase , and the expression of tandem gene duplicates was often greater than twofold [ 61 ]. Additionally, it was suggested that the relatively large number of lipase genes in the same species could be a trade-off between having sufficient catalytic diversity for rapid dietary uptake and the cost of processing DNA [ 62 ]. Therefore, the abundant tandem duplication events of lipase for fig wasps might evolve new functions, except for the lipid metabolism, which needs further studies.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Lipases in Fig Wasps (Chalcidoidea, Hymenoptera)

Wei

Wang

et al. 2022

Insects

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Lipases are the main enzymes involved in lipid metabolism. However, the characteristics of lipases in insects were scarcely investigated. Here, we screened the recently sequenced genomes of 12 fig wasp species consisting of seven pollinator fig wasps (PFWs) and five non-pollinating fig wasps (NPFWs) for the six major lipase gene families. In total, 481 lipase genes were identified, and the two most numerous families were the neutral and acid lipases. Tandem duplication accounted for the expansion of the gene family. NPFWs had significantly more lipases than PFWs. A significant gene family contraction occurred in the clade of PFWs. The difference of lipases between NPFWs and PFWs might contribute to their distinction in life histories and feeding regimes. Phylogenetic analysis showed that the lipase genes of each fig wasp species was almost equally distributed in each clade, indicating that the lipase genes were conserved. The gene structures were similar within each clade, while they were different among clades. Most of the neutral and acid lipases were signal peptides and located extracellularly. The pathways of lipases involved were predicted. This genome-wide study provides a systematic analysis of lipase gene families in 12 hymenopteran insects and further insights towards understanding the potential functions of lipases.

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Marine invertebrate lipases: Comparative and functional genomic analysis

Cited by 6 publications

References 108 publications

Recombinant expression and characterisation of a lipase from the Antarctic zooplanktonSalpa thompsoni

Recombinant expression and characterisation of a lipase from the Antarctic zooplanktonSalpa thompsoni

De novo transcriptome analysis and identification of genes associated with immunity, detoxification and energy metabolism from the fat body of the tephritid gall fly, Procecidochares utilis

Genome-Wide Identification and Analysis of Lipases in Fig Wasps (Chalcidoidea, Hymenoptera)

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