Purification and biochemical characterization of the haloalkaliphilic archaeonNatronococcus occultus extracellular serine protease

Studdert, Claudia A.; Seitz, María Karina Herrera; Gil, Maria Ines Plasencia; Sánchez, Jorge; Castro, Rosana Esther de

doi:10.1002/1521-4028(200112)41:6<375::aid-jobm375>3.0.co;2-0

Cited by 78 publications

(57 citation statements)

References 15 publications

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“…A few proteases from extreme halophiles have been reported from archaeal halophiles [171,175,176], but some from moderately halophilic and halotolerant bacteria have also been purified and studied [27,172,177,178]. As moderate halophiles are adapted to grow over a wide salt range (optimal growth at 3-15% NaCl), they represent an interesting group of organisms with great industrial potential [177].…”

Section: Alkaline Proteases From Halophilesαsupporting

confidence: 75%

“…An extracellular protease from Halobacterium halobium has been exploited for efficient peptide synthesis in water/ N-N-dimethylformamide [170]. Novel protease from haloalkaliphilic Natronococcus occultus was active in the presence of 1-2 M NaCl or KCl and lost activity below 0.5 M NaCl [171]. A protease from moderately halophilic bacterium Pseudoalteromonas and Nesterenkonia sp.…”

Section: Alkaline Proteases From Halophilesαmentioning

confidence: 99%

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Extreme Environments: Goldmine of Industrially Valuable Alkali-stable Proteases

Garg¹,

Singh²

2015

Enz Eng

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Since the advent and release of Bio 40 (the first detergent with bacterial alkaline protease) in 1959, exploration of the microbial alkaline proteases has been exploited beyond expectations. The inventory of microbial alkaline proteases is difficult to draft as it is increasing daily. Most of these proteases are the result of studies in which one or a few isolates were targeted for an alkali-stable enzyme. Although, the worldwide research on microbial alkaline proteases has been widely performed, we still need an improved enzyme capable of catalyzing reactions under extreme conditions (also called as extremozymes), e.g., high alkalinity and salinity, anhydrous environment, cold and hot environment, etc. As the search for extremozymes is in progress, it is imperative to explore the extreme environments to get some novel microbial strains (extremophiles) for alkali-stable proteases. This review article is an effort to compile the scattered information on some extremophiles and their alkali-stable proteases, which may have better specific industrial applications. It includes: (i) various extreme environments relevant to industrial biocatalysts, (ii) strategies of extremophilic microbes and enzymes which make them able to tolerate such conditions, and (iii) an overview of some important work done so far to explore industrially relevant extremophilic enzymes from extremophiles.

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Section: Alkaline Proteases From Halophilesαsupporting

confidence: 75%

Section: Alkaline Proteases From Halophilesαmentioning

confidence: 99%

Extreme Environments: Goldmine of Industrially Valuable Alkali-stable Proteases

Garg¹,

Singh²

2015

Enz Eng

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“…Extracellular proteolytic activity was also detected in the haloalkaliphilic archaeon Natronococcus occultus as the culture reached the stationary growth phase. 34) Optimal conditions for activity were attained at 60˚C and 1-2 M NaCl or KCl. Gelatin zymography in the presence of 4 M betaine revealed a complex pattern of active species with apparent molecular masses ranging from 50 to 120 kDa.…”

Section: )mentioning

confidence: 99%

Alkaliphiles

Horikoshi

2004

Proc. Jpn. Acad., Ser. B

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Abstract:The term alkaliphile is used for microorganisms that grow optimally or very well at pH values above 9, but cannot grow or grow only slowly at the near neutral pH value of 6.5. Alkaliphiles include prokaryotes, eukaryotes, and archaea. Alkaliphiles can be isolated from normal environments such as garden soil, although viable counts of alkaliphiles are higher in samples from alkaline environments. The cell surface plays a key role in keeping the intracellular pH value in the range between 7 and 8.5, allowing alkaliphiles to thrive in alkaline environments. Alkaliphiles have made a great impact in industrial applications. Biological detergents contain alkaline enzymes, such as alkaline cellulases and/or alkaline proteases that have been produced from alkaliphiles. Another important application is the industrial production of cyclodextrin with alkaline cyclomaltodextrin glucanotransferase. This enzyme reduced the production cost and paved the way for cyclodextrin use in large quantities in foodstuffs, chemicals and pharmaceuticals. It has also been reported that alkali-treated wood pulp could be biologically bleached by xylanases produced by alkaliphiles.

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“…Po2 produced protease in the range of 5-20% (w/ v) NaCl, optimally (162-170 U/ml) at 10% (w/v) NaCl 19 . Similar results have also been reflected by the haloalkalophilic archaeon, Natronococcus occultus in which protease secretion was optimum at 1-2 M NaCl 21 . However, in the case of the archaebacterium Halobacterium mediterranei, a much higher salt requirement (25%, w/v) for serine protease secretion was reported 22 .…”

Section: Resultsmentioning

confidence: 99%

Antagonistic Bacillus cereus TC-1 Isolated from Solar Salt Work in Southern India

Donio

Viji

Jenifer

et al. 2016

Bangla. J. Microbiol.

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Bacillus cereus TC-1 was isolated from condenser pond of manmade solar salt pan Thamaraikulam, Tamilnadu, India effectively suppressed the shrimp bacterial pathogens Vibrio harveyi, V. parahaemolyticus, V. anguillarum, V. alginolyticus and V. vulnificus by in vitro antagonistic assay of 9 to 15 mm of zone of inhibition. Phylogenetic analysis revealed that, B. cereus TC-1 was highly similar that the B. thuringiensis strain (100%) and other Bacillus sp. Their optimum growth between at the range of 4 to 6% of NaCl in the growth media and significantly (P<0.05) high alkaline protease production (170.85 U/ml) was observed in the NaCl of 6 %. Based on the antagonistic activity of the B. cereus TC-1 against the shrimp pathogens and its antimicrobial factors, it may be used as probiotics and developing novel antimicrobial bioactive substances against aquatic pathogens.

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Purification and biochemical characterization of the haloalkaliphilic archaeonNatronococcus occultus extracellular serine protease

Cited by 78 publications

References 15 publications

Extreme Environments: Goldmine of Industrially Valuable Alkali-stable Proteases

Extreme Environments: Goldmine of Industrially Valuable Alkali-stable Proteases

Alkaliphiles

Antagonistic Bacillus cereus TC-1 Isolated from Solar Salt Work in Southern India

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