Alkaliphiles

Horikoshi, Koki

doi:10.2183/pjab.80.166

Cited by 36 publications

(27 citation statements)

References 45 publications

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“…The diluted samples were spread plated on Horikoshi medium I [15] and plates were incubated at 30°C for 72 h. Morphologically distinct strains were subcultured and screened for protease production on Horikoshi medium I supplemented with 1% (w/v) skim milk. The pure cultures were preserved as slants at 4°C and as glycerol suspensions (20%, v/v) at -20°C.…”

Section: Isolation Of Alkaliphilic Bacteriamentioning

confidence: 99%

Optimization of Alkaline Protease Production by Alkaliphilic Bacillus sp. KW2 in Low Cost Medium using Statistical Approaches

Kshetri¹,

Ningombam²

2016

Appli Micro Open Access

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Objective: Isolation of alkaline protease producing alkaliphilic bacteria and optimization of protease production in low cost medium.Methods: Optimization of protease production was performed by one-variable-at-a-time (OVAAT) approach and statistical approaches using Design Expert 6 software.Results: Nine (9) morphologically distinct alkaliphilic bacterial strains were isolated. Of these isolates 6 strains were found to be positive for protease production. Among these proteolytic strains, Bacillus sp. KW2 was selected for protease production optimization studies. The optimal protease production was observed at 30°C and pH 10.7. Among the various carbon and nitrogen sources studied rice bran and soybean meal were found to be the best Carbon and Nitrogen sources respectively. Plackett-Burman design (PBD) was used to screen signal factors that influenced the protease production. Rice bran, soybean meal, inoculum age, CaCl 2 and inoculum size gave positive effects whereas KH 2 PO 4 and MgSO 4 gave negative effects on protease production. The four most critical factors viz. rice bran, soybean meal, CaCl 2 and KH 2 PO 4 were selected for optimization studies by Response Surface Methodology (RSM). The response surface graphs showed significant interactions among the factors and a final optimized medium (FOM) was obtained after PBD and RSM experiments. Overall, a 4.8 fold increase in protease production was observed after optimization. Studies of the time course of protease production by Bacillus sp. KW2 revealed that maximal protease production occurred at the stationary phase (84 h) of growth.Conclusions: The present study shows that protease production is greatly influenced by cultural conditions and media constituents. This study also established that the strain KW2 could produce alkaline protease in a low cost medium containing rice bran and soybean meal as major carbon and nitrogen sources. KW2 and its protease may be promising agents for biotechnological applications.

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Section: Isolation Of Alkaliphilic Bacteriamentioning

confidence: 99%

Optimization of Alkaline Protease Production by Alkaliphilic Bacillus sp. KW2 in Low Cost Medium using Statistical Approaches

Kshetri¹,

Ningombam²

2016

Appli Micro Open Access

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“…For example, soils constitute a highly heterogeneous matrix, in contrast to aquatic ecosystems such as the one investigated in this study, which may explain the large abundance of alkaliphiles [7] and halophiles [8] in neutral and non-saline soils.…”

Section: Introductionmentioning

confidence: 97%

Communities that thrive in extreme conditions captured from a freshwater lake

et al. 2016

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Organisms that can grow in extreme conditions would be expected to be confined to extreme environments. However, we were able to capture highly productive communities of algae and bacteria capable of growing in acidic ( pH 2), basic ( pH 12) and saline (40 ppt) conditions from an ordinary freshwater lake. Microbial communities may thus include taxa that are highly productive in conditions that are far outside the range of conditions experienced in their host ecosystem. The organisms we captured were not obligate extremophiles, but were capable of growing in both extreme and benign conditions. The ability to grow in extreme conditions may thus be a common functional attribute in microbial communities.

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“…Moreover, alkaliphilic Bacillus spp. also constitute an important source of alkaline proteases (Ito et al 1998 ;Horikoshi 2004 ). Since the alkaliphilic Bacillus group was classifi ed into nine species (Nielsen et al 1995 ), numerous novel strains have been described and many of them are alkaline protease producers (Saeki et al 2002 ; Manni et al ( 2008 ) N.L.…”

Section: Bacterial Alkaline Proteasesmentioning

confidence: 99%

“…The alkaliphilic Bacillus group constitutes an important source of extracellular enzymes for numerous industrial processes (Ito et al 1998 ;Horikoshi 2004 ). In PAT 05…”

Section: The Patagonian Montementioning

confidence: 99%

Alkaline Proteases from Patagonian Bacteria

Olivera

Iglesias

Sequeiros

2016

Biology and Biotechnology of Patagonian Microorganisms

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In addition to their ecological importance in the acquisition of nitrogenrich organic compounds, extracellular proteases also have interesting biotechnological applications. Particularly, alkaline proteases represent one of the most important groups of commercial enzymes. First, we introduce the classifi cation and catalytic mechanisms of proteases. Then, this chapter reviews the advances in the bioprospection of alkaline proteases produced by bacteria adapted to selective conditions from different environments of Patagonia (Argentina). Among them, the arid soils of the Patagonian Monte are propitious for the development of alkaliphilic microorganisms. Thus, we focus on the description of the species Bacillus patagoniensis and the biochemical and catalytic properties of its alkaline protease. Then, we discuss investigations about alkaline protease-producing bacteria from the southern Patagonian coast, the prevalence of psychrophilic and psychrotolerant strains, and the response of their extracellular proteases to temperature.

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Alkaliphiles

Cited by 36 publications

References 45 publications

Optimization of Alkaline Protease Production by Alkaliphilic Bacillus sp. KW2 in Low Cost Medium using Statistical Approaches

Optimization of Alkaline Protease Production by Alkaliphilic Bacillus sp. KW2 in Low Cost Medium using Statistical Approaches

Communities that thrive in extreme conditions captured from a freshwater lake

Alkaline Proteases from Patagonian Bacteria

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