Purification and biochemical characterization of two extracellular peroxidases from Phanerochaete chrysosporium responsible for lignin biodegradation

Zeng, Guangming; Zhao, Meihua; Huang, Danlian; Lai, Cui; Huang, Chao; Wei, Zhen; Xu, Piao; Li, Ningjie; Zhang, Chen; Li, Fang‐Ling; Cheng, Min

doi:10.1016/j.ibiod.2013.07.005

Cited by 52 publications

(33 citation statements)

References 40 publications

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“…2). This result further proved that MnP is a lignin attack enzyme produced by basidiomycetes in the initial stage of lignin degradation [20]. On the other hand, a relatively higher Lac activity (254.72 U · L −1 ) was also observed with T. hirsute .…”

Section: Resultssupporting

confidence: 56%

Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

He²,

Shi

et al. 2016

BMC Biotechnol

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BackgroundTobacco stalk is one kind of abundant crop residues in China. The high lignification of tobacco stalk increases its reusing cost and the existing of nicotine will cause serious pollution. The biodegradation of lignocellulosic biomass has been demonstrated to be an environmental and economical approach for the utilization of plant stalk. Meanwhile, many nicotine-degrading microorganisms were found in nature. However, microorganisms which could degraded both nicotine and lignin haven’t been reported. Therefore, it’s imperative to find some suitable microorganisms to break down lignin and simultaneously remove nicotine in tobacco stalk.ResultsThe nicotine in tobacco stalk could be degraded effectively by Trametes versicolor, Trametes hirsute and Phanerochaete chrysosporium. The nicotine content in tobacco stalk was lowered to below 500 mg/kg (a safe concentration to environment) after 10 days of fermentation with Phanerochaete chrysosporium and Trametes versicolor, and 15 days with Trametes hirsute. The degradation rate of lignin in the fermented tobacco stalk was 37.70, 51.56 and 53.75% with Trametes versicolor, Trametes hirsute and Phanerochaete chrysosporium, respectively. Meanwhile, 24.28% hemicellulose was degraded by Phanerochaete chrysosporium and 28.19% cellulose was removed by Trametes hirsute. Through the enzyme activity analysis, the main and highest ligninolytic enzymes produced by Phanerochaete chrysosporium, Trametes hirsute and Trametes versicolor were lignin peroxidase (88.62 U · L−1), manganese peroxidase (100.95 U · L−1) and laccase (745.65 U · L−1). Meanwhile, relatively high and stable cellulase activity was also detected during the fermentation with Phanerochaete chrysosporium, and the highest endoglucanase, exoglucanase and filter paper enzyme activities were 0.38 U · mL−1, 0.45 U · mL−1 and 0.35U · mL−1, respectively. Moreover, the products in the fermentation of tobacco stalk with P. chrysosporium were identified with GC-MS, besides the chemicals produced in the degradation of lignin and nicotine, some small molecular valuable chemicals and fatty acid were also detected.ConclusionsOur study developed a new method for the degradation and detoxification of tobacco stalk by fermentation with white rot fungi Phanerochaete chrysosporium and Trametes hirsute. The different oxidative enzymes and chemical products detected during the degradation indicated a possible pathway for the utilization of tobacco stalk.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-016-0311-8) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 56%

Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

He²,

Shi

et al. 2016

BMC Biotechnol

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“…Membranes of 10 kDa are often utilized to concentrate both crude and purified peroxidase extract by chromatography techniques . Gottschalk et al .…”

Section: Resultsmentioning

confidence: 99%

“…Generally, therapeutic applications require high purity peroxidase, whereas certain industrial large‐scale applications allow the use of crude or partially purified enzyme extract. Peroxidase purification can improve thermal stability and substrate degradation capacity . Peroxidases have been purified from a number of organisms including bacteria, fungi and plants …”

Section: Introductionmentioning

confidence: 99%

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Gautério

Malta

Reginatto

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Once peroxidase purification can improve substrate degradation capacity, peroxidase from rice bran was purified by expanded bed adsorption (EBA) coupled to diafiltration/ultrafiltration (DF/UF) and applied for deoxynivalenol (DON) reduction. RESULTS The DF/UF step was optimized according to membrane molecular weight cutoff (MWCO), DF cycles number, operation temperature and CaCl2 addition to the DF buffer. DON reduction tests were carried out with both clarified and purified peroxidase. The use of MWCO of 10 kDa, 10 °C, six DF cycles and adding 4 mmol L−1 CaCl2 to DF buffer promoted enzyme purification of 4.4‐fold and enzymatic recovery of 79.4%. The global purification process combining two techniques resulted in enzyme purification of 75.1‐fold with an enzymatic recovery of 22.8%. Peroxidase purified by EBA combined with DF/UF resulted in DON reduction of 81.7% in a model system. CONCLUSION Peroxidase purification was achieved by scale‐up integrated strategies. The use of purified peroxidase from agricultural by‐products could be a promising alternative for enzymatic DON levels reduction. © 2017 Society of Chemical Industry

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“…Therefore, the degradation of lignin is the key step to the lignocellulose transformation (Huang et al, 2010;Zhao et al, 2012). Phanerochaete chrysosporium (P. chrysosporium), a well-known white-rot fungus, has been shown to effectively degrade lignin, which has been correlated with the simultaneous secretion of extracellular oxidative enzymes including lignin peroxidase (LiP) and manganese peroxidase (MnP) (Kersten and Cullen, 2007;Zeng et al, 2013). Therefore, much attention has currently been drawn to the development of solid-state fermentation (SSF) with P. chrysosporium for the efficient treatment of lignocellulosic waste (Huang et al, 2008;Ganesh Kumar et al, 2006;Zeng et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Growth, metabolism of Phanerochaete chrysosporium and route of lignin degradation in response to cadmium stress in solid-state fermentation

et al. 2015

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Purification and biochemical characterization of two extracellular peroxidases from Phanerochaete chrysosporium responsible for lignin biodegradation

Cited by 52 publications

References 40 publications

Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

Biodegradation of lignin and nicotine with white rot fungi for the delignification and detoxification of tobacco stalk

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Growth, metabolism of Phanerochaete chrysosporium and route of lignin degradation in response to cadmium stress in solid-state fermentation

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