Purification and biochemical characterization of glutathione S-transferases from three strains of Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae): Implication of insecticide resistance

Dou, Wei; Wu, Shuang; Hassan, Muhammad Waqar; Wang, Jinjun

doi:10.1016/j.pestbp.2009.02.005

Cited by 20 publications

(12 citation statements)

References 33 publications

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“…The GST kinetic constants for the four field populations of B. dorsalis toward CDNB and GSH were comparable to values reported from several insect species (Valles et al, 2003;Yamamoto et al, 2008;Dou et al, 2009;Wu et al, 2009). With CDNB as the substrate, the K m values of the purified GSTs in the DG and HN populations are significantly higher than those for the GZ and YN populations, suggesting a lower affinity of GSTs toward the substrates in the DG and HN populations of B. dorsalis.…”

Section: Discussionsupporting

confidence: 76%

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Dou

Wang

et al. 2011

Arch Insect Biochem Physiol

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Glutathione S-transferases (GSTs) are a group of detoxification enzymes that catalyze the nucleophilic addition of glutathione to a wide variety of endogenous and xenobiotic compounds. In this study, GSTs were purified from four field populations of Bactrocera dorsalis with different insecticide susceptibilities by glutathione-agarose affinity chromatography. The populations were collected from Dongguan (DG) and Guangzhou (GZ) of the Guangdong Province, Haikou of the Hainan province (HN), and Kunming of the Yunnan province (YN), China. Differences in GST characteristics among the four populations were studied using purified enzyme samples through comparative SDS-PAGE, kinetic, and inhibition experiments. The specific activities of the purified enzymes were similar, but the purification yield of the GZ population (31.54%) was the lowest. SDS-PAGE analysis showed only one band at approximately 23 kDa for these four populations. Kinetic analyses showed that the affinities of the purified GSTs from the GZ and YN populations for 1-chloro-2.4-dinitrobenzene (CDNB) were much higher than those of GSTs from the other two populations, whereas the HN population had the highest catalytic capability in terms of V(max) value. The optimum temperature for CDNB conjugation was 37 °C and the optimum pH was 7.5 in all four populations. Inhibition kinetics showed that ethacrynic acid, diethyl maleate, tetraethylthiuram disulfide, curcumin, bromosulfalein, and β-cypermethrin had excellent inhibitory effects on GSTs in the four populations of B. dorsalis, but the low inhibitory effects of malathion and avermectin did not differ between populations. These results suggest that GSTs may have a role in detoxification of β-cypermethrin in B. dorsalis.

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Section: Discussionsupporting

confidence: 76%

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Dou

Wang

et al. 2011

Arch Insect Biochem Physiol

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“…Quantitative determination of the total protein content was carried out by the Bradford method [21,22]. Protein concentrations were determined using a spectrophotometer (UV-2102PC, UNICO Instrument Co. Ltd., Shanghai, China) at 595 nm and bovine serum albumin (BSA) as standard.…”

Section: Methodsmentioning

confidence: 99%

Optimization of affinity partitioning conditions of papain in aqueous two-phase system using response surface methodology

Ling

Nie

et al. 2010

Separation and Purification Technology

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“…In insects, the diverse functions of P450 enzymes range from the synthesis and degradation of ecdysteroids and juvenile hormones to the xenobiotics metabolism (Feyereisen, 2005). Insect GSTs have been implicated in resistance to insecticides, organophosphorous and pyrethroid, through direct insecticide metabolism (Wei et al, 2001) or by protecting against secondary toxic effects, such as increases in lipid peroxidation, induced by insecticide exposure (Dou et al, 2009). A member of the esterase cluster probably plays a role in the detoxifcation of xenobiotic esters.…”

Section: Introductionmentioning

confidence: 99%

Toxicity and biochemical efficacy of six essential oils against Tribolium confusum (du val) (Coleoptera: Tenebrionidae)

El-Aziz

El-Sayed

2009

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Six essential oils extracted from garlic (Allium sativum), mint (Mentha piperitaa), basil (Ocimum basilicum), thyme (Thymus vulgaris) , sesame (Sesamum Indicume) and chamomile (Chamaemelum nobile) were tested for both repellency and toxicity against the confused flour beetle, Tribolium confusum adults and last larval instar. Two kinds of repellency bioassays were performed by using filter paper tests and treated kernels. The toxicological bioassay was performed by topical application. The results showed that the tested oils were highly repellent to the adult insect compared to the larvae. By contrast, the toxicological bioassay showed that larvae were more susceptible to tested essential oils than the adults. Effects of essential oils on five detoxification enzymes were also investigated. A reduction in total activities of the major detoxification enzymes in treated larvae was also observed.

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Purification and biochemical characterization of glutathione S-transferases from three strains of Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae): Implication of insecticide resistance

Cited by 20 publications

References 33 publications

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Purification and biochemical characterization of glutathione S‐transferases from four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Optimization of affinity partitioning conditions of papain in aqueous two-phase system using response surface methodology

Toxicity and biochemical efficacy of six essential oils against Tribolium confusum (du val) (Coleoptera: Tenebrionidae)

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