Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Sabullah, Mohd Khalizan; Shukor, Mohd Yunus; Shamaan, Nor Aripin; Khalid, Arif; Gansau, Azlan Jualang; Sulaiman, Mohd Rosni; Jirangon, Hussain; Ahmad, Siti Aqlima

doi:10.17957/ijab/15.0012

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…Hence, the lowest MW appeared first throughout the gel. The lowest MW recorded is in accordance with that obtained by Sabullah et al (2015d) and Ahmad et al (2016a, b, c); the extracted ChE from two different freshwater fish species known as A. testudineus and P. javanicus. In addition, whole blood including blood plasma and serum is usually predominant with acetylcholinesterase and pseudocholinesterase.…”

Section: In Vitro Analysis Of O Mossambicus Blood Cholinesterase Expsupporting

confidence: 90%

In vivo and in vitro effects on cholinesterase of blood of Oreochromis mossambicus by copper

et al. 2019

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The present study is aimed to evaluate the effects of sub-acute toxicity testing of copper sulphate (CuSO 4), on behavioural, histological and biochemical changes of the Oreochromis mossambicus (black tilapia) blood tissues. The effects were assessed according to the previous results on sub-acute toxicity test after exposing fish to several concentrations (0.0, 2.5, 5.0, and 10.0 mg/L). The observations of scanning electron microscope, and transmission electron microscope studies revealed severe histopathological changes on the surface and the cellular changes in blood tissues, respectively. The morphological alterations in blood involved irregular structure of red blood cell and blood clot formation. CuSO 4 affected the biochemical alteration of the blood cholinesterase also known as serum cholinesterase (ChE). Blood ChE inhibited up to 80% of activity when exposed to 10.0 mg/L CuSO 4. The findings from this study can further improve the quality standards of aquaculture industry and the fundamental basis in selecting suitable strains among freshwater fish species to be used as bioindicator.

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Section: In Vitro Analysis Of O Mossambicus Blood Cholinesterase Expsupporting

confidence: 90%

In vivo and in vitro effects on cholinesterase of blood of Oreochromis mossambicus by copper

et al. 2019

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“…Hence, the preliminary evaluation of water quality is crucial for utilisation in everyday life. Equipped with contemporary automation such as High-Performance Liquid Chromatography (HPLC) and Inductively Coupled Plasma (ICP) in quantifying toxicants level, these inventions are costly, time-consuming and require expertise for handling (Sabullah et al, 2015). Therefore, alternatively, biosensor using enzyme can be used as a preliminary screening to detect contaminants level semiquantitatively.…”

Section: Introductionmentioning

confidence: 99%

Cholinesterase from the Liver of Diodon hystrix for Detection of Metal Ions

Nordin¹,

Cletus²,

Sabullah³

et al. 2020

JST

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The discharge of industrial effluents into nearby water bodies affects the inhabitants including living organisms. The presence of foreign materials such as heavy metals can be a threat to the ecosystem as they are enormously carcinogenic even though in minute concentration. Hence, an economical and time-efficient preliminary screening test is crucial to be developed for the detection of heavy metals, prior to employment of high technology instruments. In this study, cholinesterase (ChE) from Sabah porcupine fish, Diodon hystrix was purified to test for its potential as an alternative biosensor in detecting metal ions. Few enzymatic parameters including specificity of substrate, temperature and pH were applied to determine its optimal enzymatic activity. ChE enzyme was found to be more sensitive towards the presence of substrate, butyrylthiocholine iodide (BTC), in contrast to acetylthiocholine iodide (ATC) and propionylthiocholine iodide (PTC) with the effective coefficient at 7193, 3680.15 and 2965.26 Vmax/Km, respectively. Moreover, the extracted ChE enzyme showed the optimum activity at pH 9 of 0.1 M Tris-HCl and at 25°C to 30°C range of temperature. When subjected to heavy metals, ChE enzyme was significantly inhibited as the enzyme activity was reduced in the sequence of Hg > Ag > Cr > Cu > Cd > Pb ≥ Zn > As. As a conclusion, the partially purified ChE enzyme proved its sensitivity towards metal ion exposure and can be used as an alternative method in screening the level of contamination in the environment.

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“…Homogenisation was carried out using Ultra-Turrax T25 Homogenizer. Homogenate was then centrifuged at 10,000 xg in a Sorvall Ultra Pro-TH-641 centrifuge for 20 min at 4°C (Sabullah et al, 2015a). The supernatant was collected and stored at -25°C in clean polycarbonate tubes.…”

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confidence: 99%

Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium

Faddzil¹,

Ahmad²,

Yasid³

et al. 2019

JEB

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Cholinesterase (ChE) inhibition and histopathological features of brain tissues of Clarias gariepinus were investigated with a purpose to develop biomarkers for monitoring heavy metals. The effects of cadmium toxicity on were assessed by ChE inhibition and characterisation of ChE by affinity chromatography and histological variations in brain tissues were studied by H&E staining under light microscope.

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Purification and Anticholinesterase Sensitivity of Cholinesterase Extracted from Liver Tissue of Puntius Javanicus

Cited by 4 publications

References 40 publications

In vivo and in vitro effects on cholinesterase of blood of Oreochromis mossambicus by copper

In vivo and in vitro effects on cholinesterase of blood of Oreochromis mossambicus by copper

Cholinesterase from the Liver of Diodon hystrix for Detection of Metal Ions

Characterisation of cholinesterase and histopathological features of brain of Clarias gariepinus following exposure to cadmium

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