Pure Culture of Anabaena flos-aquae A-37

Tischer, R. G.

doi:10.1038/205419a0

Cited by 32 publications

(15 citation statements)

References 7 publications

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“…The Eis_ Ava protein, containing a N-terminal His 6 -tag, was overexpressed and purified using the pEis_ Ava –pET28a overexpression construct as previously reported for its homologue in Mycobacterium tuberculosis (Eis_ Mtb ). 3 The Eis_ Ava protein was dialyzed in Tris–HCl buffer (50 mM, pH 7.5 adjusted at rt), flash frozen in the dialysis buffer containing 10% glycerol, and stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Cyanobacteria do not cause any clinical infections that are treated with AGs. However, the A. variabilis ATCC 29413 strain was isolated from a sewage oxidation pond, 6 and the development of resistance to AGs through water contamination has been previously documented in various bacterial species. 7 Thus, exposure to AGs through sewage waste provides a potential explanation as to why a putative AG-acetylating enzyme, Eis_ Ava , has arisen in this cyanobacterial strain.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis

et al. 2012

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The Mycobacterium tuberculosis enhanced intracellular survival (Eis_Mtb) protein is a clinically important aminoglycoside (AG) multi-acetylating enzyme. Eis homologues are found in a variety of mycobacterial and non-mycobacterial species. Variation of the residues lining the AG-binding pocket and positions of the loops bearing these residues in the Eis homologues dictates the substrate specificity and, thus, Eis homologues are Nature-made tools for elucidating principles of AG recognition by Eis. Here, we demonstrate that the Eis from Anabaena variabilis (Eis_Ava), the first non-mycobacterial Eis homologue reported, is a multi-acetylating AG-acetyltransferase. Eis_Ava, Eis from Mycobacterium tuberculosis (Eis_Mtb), and Eis from Mycobacterium smegmatis (Eis_Msm) have different structures of their AG-binding pockets. We perform comparative analysis of these differences and investigate how they dictate the substrate and cosubstrate recognition and acetylation of AGs by Eis.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis

et al. 2012

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“…B . JENSEN (Falkenberg et a/., 1972;Booker 8i Walsby, 1979) that the correct nomenclature for this strain [A-37 of.Tischer (Tischer, 1965), UTEX 1444, ATCC 294131 is Anabaena variabilis and this name is used here.…”

Section: Methodsmentioning

confidence: 99%

Energy Requirement for Diazotrophic Growth of the Cyanobacterium Anabaena variabilis Determined from Growth Yields in the Dark

Jensen¹

1983

Microbiology

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The cyanobacterium Anabaena variabilis CCAP 1403/13a (UTEX 1444, ATCC 2941 3) was grown diazotrophically in the dark in the presence of fructose with a doubling time of 25 h at 35 "C. This was 40% of the maximum rate observed in the light. Growth rates in the dark were similar with nitrate or ammonium as nitrogen sources or under diazotrophic conditions. Darkgrown cyanobacteria reduced acetylene in the dark at 25% of the rate observed for photoautotrophic cultures in the light. Heterotrophic growth yields for dark growth with different nitrogen sources were estimated from the extent of fructose-dependent growth in batch cultures. The ratio between cell carbon and fructose carbon was 0.38 in diazotrophically grown cells, 0.38 with nitrate and 0.52 with ammonia. Thus the energy requirement for growth on molecular nitrogen is similar to the energy requirement for growth on nitrate, but greater than that for growth on ammonia. Since the diazotrophic culture always had a dissolved O2 concentration of at least 80% of air saturation, this demonstrates that there are no detectable extra energy requirements for aerobic nitrogen fixation compared to nitrate reduction, such as would result from a need for respiratory protection of nitrogenase. t Present address :

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“…The inhibition has been often attributed to various extracellular products including polysaccharides (Biswas, 1957Bishop, Adams & Hughes, 1957Tischer, 1965 ;Moore & Tischer, 1965) and nitrogenous compounds (Watanabe, 1951 ;Fogg, 1952Fogg, . 1962Goryunova & Rzhanova, 1964;Fay, Kumar & Fogg, 1964;Gorham, 1964 ;Walsby, 1965 ; Dr. W. Junk b.v .…”

Section: Introductionmentioning

confidence: 99%

Growth interactions among blue-green (Anabaena Oscillarioides, Microcystis aeruginosa) and green (Chlorella sp.) algae

Lam

Silvester

1979

Hydrobiologia

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The growth interactions amongst the blue-green algal species Anabaena oscillarioides, Microcystis aeruginosa and the green alga, Chlorella sp . were studied both in mixed cultures and in filter cultures separated by a membrane filter in the two arms of an interaction U-tube . The role of nutrients especially phosphate upon the interaction has also been studied .Anabaena and Microcystis both inhibited the growth of Chlorella while Microcystis also inhibited the growth of Anabaena. The inhibitory effect of Microcystis was found to be dependent on high concentrations of the initial algal inocula and independent of the initial concentration of nutrients such as inorganic phosphate, indicating that the nature of the inhibition is probably due to the production of inhibitory extracellular products by Microcystis . On the other hand, the inhibitory effect of Anabaena on Chlorella is the consequence of nutrient competition with Anabaena competing more effectively for the available phosphate .

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Pure Culture of Anabaena flos-aquae A-37

Cited by 32 publications

References 7 publications

Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis

Biochemical and structural analysis of aminoglycoside acetyltransferase Eis from Anabaena variabilis

Energy Requirement for Diazotrophic Growth of the Cyanobacterium Anabaena variabilis Determined from Growth Yields in the Dark

Growth interactions among blue-green (Anabaena Oscillarioides, Microcystis aeruginosa) and green (Chlorella sp.) algae

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