2012
DOI: 10.1105/tpc.112.096438
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Pulsing of Membrane Potential in Individual Mitochondria: A Stress-Induced Mechanism to Regulate Respiratory Bioenergetics in Arabidopsis

Abstract: Mitochondrial ATP synthesis is driven by a membrane potential across the inner mitochondrial membrane; this potential is generated by the proton-pumping electron transport chain. A balance between proton pumping and dissipation of the proton gradient by ATP-synthase is critical to avoid formation of excessive reactive oxygen species due to overreduction of the electron transport chain. Here, we report a mechanism that regulates bioenergetic balance in individual mitochondria: a transient partial depolarization… Show more

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Cited by 108 publications
(129 citation statements)
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References 73 publications
(98 reference statements)
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“…We decided, therefore, to investigate membrane potential dynamics in friendly. Transient changes in membrane potential, which we term pulses (Schwarzländer et al, 2012b), are rare events in the wild type; thus, it was immediately evident from confocal scanning laser microscopy (CSLM) imaging that the number of mitochondria exhibiting pulses and the frequency of pulses were greater in friendly than in the wild type (Fig. 6A).…”
Section: Friendly Is a Cytosolic Protein With Either A Diffuse Expresmentioning
confidence: 99%
See 2 more Smart Citations
“…We decided, therefore, to investigate membrane potential dynamics in friendly. Transient changes in membrane potential, which we term pulses (Schwarzländer et al, 2012b), are rare events in the wild type; thus, it was immediately evident from confocal scanning laser microscopy (CSLM) imaging that the number of mitochondria exhibiting pulses and the frequency of pulses were greater in friendly than in the wild type (Fig. 6A).…”
Section: Friendly Is a Cytosolic Protein With Either A Diffuse Expresmentioning
confidence: 99%
“…Green and red channels were captured simultaneously preconversion and postconversion using a 488-nm laser at 1% and a 561-nm laser at 1.5%, respectively. CSLM time courses were saved as .avi files and analyzed using a custom-designed Matlab program (Schwarzländer et al, 2012b). Green-red ratios were calculated for an identical number of pixels covered by each identified mitochondrion in each of the 74 movie frames of each biological replicate.…”
Section: In Vivo Mitochondrial Fusion Assaymentioning
confidence: 99%
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“…While the pH gradient across the IM has long been assumed to be relatively small and constant, this view is currently changing (189,198). In Arabidopsis roots, matrix pH can transiently increase, due to an increased pH gradient (196,197). Similar events have been observed in mammalian mitochondria (9,189).…”
Section: Nadmentioning
confidence: 74%
“…Homologs are present in plants with predicted mitochondrial targeting (207). Rapid changes in free matrix calcium referred to as pulses coincide with a rapid bioenergetic remodeling in single mitochondria of Arabidopsis (197). Excessive matrix calcium load can trigger cell death signaling in plants (122).…”
Section: Nadmentioning
confidence: 99%