Pulsed Laser Photo-Crosslinking of Gelatin Methacryloyl Hydrogels for the Controlled Delivery of Chlorpromazine to Combat Antimicrobial Resistance

Tozar, Tatiana; Nistorescu, Simona; Boni, Mihai; Pîrcălăbioru, Grațiela Grădișteanu; Neguț, Irina; Staicu, Angela

doi:10.3390/pharmaceutics14102121

Cited by 7 publications

(3 citation statements)

References 66 publications

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“…As GelMA exhibits great compatibility with various cells, biodegradability, and accessibility ( Loessner et al 2016 ), it is widely used in hard and soft tissue engineering ( Li et al 2018 ; Chimene et al 2020 ; Unagolla and Jayasuriya, 2020 ; Rajabi et al 2021 ). Besides, due to its similarity to the main component in the extracellular matrix, GelMA has been used in drug and gene delivery, as well as in wound healing applications ( Yue et al 2015 ; Ayoub et al 2022 ; Hölzl et al 2022 ; Moniruzzaman et al 2022 ; Tozar et al 2022 ). As one of the best 3D bioprinting materials, GelMA still has some limitations, such as low viscosity at room or higher temperature and rapid degradation in body tissue ( Seyedmahmoud et al 2019 ; Rajabi et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Global hotspots and emerging trends in 3D bioprinting research

Ding

Tang

Huang

et al. 2023

Front. Bioeng. Biotechnol.

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Three-dimensional (3D) bioprinting is an advanced tissue engineering technique that has received a lot of interest in the past years. We aimed to highlight the characteristics of articles on 3D bioprinting, especially in terms of research hotspots and focus. Publications related to 3D bioprinting from 2007 to 2022 were acquired from the Web of Science Core Collection database. We have used VOSviewer, CiteSpace, and R-bibliometrix to perform various analyses on 3,327 published articles. The number of annual publications is increasing globally, a trend expected to continue. The United States and China were the most productive countries with the closest cooperation and the most research and development investment funds in this field. Harvard Medical School and Tsinghua University are the top-ranked institutions in the United States and China, respectively. Dr. Anthony Atala and Dr. Ali Khademhosseini, the most productive researchers in 3D bioprinting, may provide cooperation opportunities for interested researchers. Tissue Engineering Part A contributed the largest publication number, while Frontiers in Bioengineering and Biotechnology was the most attractive journal with the most potential. As for the keywords in 3D bioprinting, Bio-ink, Hydrogels (especially GelMA and Gelatin), Scaffold (especially decellularized extracellular matrix), extrusion-based bioprinting, tissue engineering, and in vitro models (organoids particularly) are research hotspots analyzed in the current study. Specifically, the research topics “new bio-ink investigation,” “modification of extrusion-based bioprinting for cell viability and vascularization,” “application of 3D bioprinting in organoids and in vitro model” and “research in personalized and regenerative medicine” were predicted to be hotspots for future research.

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Section: Discussionmentioning

confidence: 99%

Global hotspots and emerging trends in 3D bioprinting research

Ding

Tang

Huang

et al. 2023

Front. Bioeng. Biotechnol.

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“…Researchers are continuously investigating various formulations based on GelMA hydrogels for drug delivery to combat diverse challenges. For example, Doxorubicin (DOX) loaded alginate-GelMA/silver nanowire conductive hydrogel [76], DOX loaded with chitosan/gelatin/dextran and polyacrylamide [77], GelMA hydrogel loaded with aluminosilicate clay nanotubes incorporated with sodium hypochlorite [78], Metformin-loaded nanosilicate/GelMA [79], Chlorpromazine-incorporated GelMA photo-crosslinked with pulsed laser [80], Cefazolin-incorporated GelMA [81], poly (n-(4aminophenyl) MA) carbon nano-onions with γ-cyclodextrin/DOX-complex reinforced GelMA [82], Artemisinin-incorporated mPEG(polyethylene glycol)-polycaprolactone (PCL) NP-based GelMA [83] are some of the prepared formulations that have shown favorable outcomes in drug delivery.…”

Section: Curcuminmentioning

confidence: 99%

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Pramanik,

Alhomrani,

Alamri

et al. 2024

Biomed. Mater.

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Gelatin methacryloyl (GelMA) hydrogels have gained significant recognition as versatile biomaterials in the biomedical domain. GelMA hydrogels emulate vital characteristics of the innate extracellular matrix (ECM) by integrating cell-adhering and matrix metalloproteinase-responsive peptide motifs. These features enable cellular proliferation and spreading within GelMA-based hydrogel scaffolds. Moreover, GelMA displays flexibility in processing, as it experiences crosslinking when exposed to light irradiation, supporting the development of hydrogels with adjustable mechanical characteristics. The drug delivery landscape has been reshaped by GelMA hydrogels, offering a favorable platform for the controlled and sustained release of therapeutic actives. The tunable physicochemical characteristics of GelMA enable precise modulation of the kinetics of drug release, ensuring optimal therapeutic effectiveness. In tissue engineering, GelMA hydrogels perform an essential role in the design of the scaffold, providing a biomimetic environment conducive to cell adhesion, proliferation, and differentiation. Incorporating GelMA in three-dimensional printing further improves its applicability in drug delivery and developing complicated tissue constructs with spatial precision. Wound healing applications showcase GelMA hydrogels as bioactive dressings, fostering a conducive microenvironment for tissue regeneration. The inherent biocompatibility and tunable mechanical characteristics of GelMA provide its efficiency in the closure of wounds and tissue repair.GelMA hydrogels stand at the forefront of biomedical innovation, offering a versatile platform for addressing diverse challenges in drug delivery, tissue engineering, and wound healing. This review provides a comprehensive overview, fostering an in-depth understanding of GelMA hydrogel’s potential impact on progressing biomedical sciences.

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“…The methacrylation of gelatin was confirmed using the FTIR-ATR and 1 H NMR. GelMA FTIR spectrum showed its characteristic stretching vibration CO (amide I) at 1630 cm –1 , deformation vibration C–N–H at 1544 cm –1 , N–H (amide II) at 1237 cm –1 , C–H bond at 1450 cm –1 , and C–N stretching vibration within amide III at 1335 cm –1 (Figure A) . The NMR spectrum showed double peaks at 6.2 and 5.7 ppm for vinyl (−CH 2 ) protons and a single peak at 1.9 ppm for methyl (−CH 3 ) proton of methacrylate, as shown in Supporting Information, Figure S1, the characteristic signals of the methacrylate group .…”

mentioning

confidence: 99%

Mechanical Properties Affect Primary T Cell Activation in 3D Bioprinted Hydrogels

et al. 2023

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T cells play a critical role in the adaptive immune response of the body, especially against intracellular pathogens and cancer. In vitro, T cell activation studies typically employ planar (two-dimensional, 2D) culture systems that do not mimic native cell-to-extracellular matrix (ECM) interactions, which influence activation. The goal of this work was to study T cell responses in a cell line (EL4) and primary mouse T cells in three-dimensional (3D) bioprinted matrices of varied stiffness. Cell-laden hydrogels were 3D bioprinted from gelatin methacryloyl (GelMA) using a digital light processing (DLP)-based 3D bioprinter operated with visible light (405 nm). Mechanical characterization revealed that the hydrogels had pathophysiologically relevant stiffnesses for a lymph node-mimetic tissue construct. EL4, a mouse T cell lymphoma line, or primary mouse T cells were 3D bioprinted and activated using a combination of 10 ng/mL of phorbol myristate acetate (PMA) and 0.1 μM of ionomycin. Cellular responses revealed differences between 2D and 3D cultures and that the biomechanical properties of the 3D bioprinted hydrogel influence T cell activation. Cellular responses of the 2D and 3D cultures in a soft matrix (19.83 ± 2.36 kPa) were comparable; however, they differed in a stiff matrix (52.95 ± 1.36 kPa). The fraction of viable EL4 cells was 1.3-fold higher in the soft matrix than in the stiff matrix. Furthermore, primary mouse T cells activated with PMA and ionomycin showed 1.35-fold higher viable cells in the soft matrix than in the stiff matrix. T cells bioprinted in a soft matrix and a stiff matrix released 7.4-fold and 5.9-fold higher amounts of interleukin-2 (IL-2) than 2D cultured cells, respectively. Overall, the study demonstrates the changes in the response of T cells in 3D bioprinted scaffolds toward engineering an ex vivo lymphoid tissue-mimetic system that can faithfully recapitulate T cell activation and unravel pathophysiological characteristics of T cells in infectious biology, autoimmunity, and cancers.

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Pulsed Laser Photo-Crosslinking of Gelatin Methacryloyl Hydrogels for the Controlled Delivery of Chlorpromazine to Combat Antimicrobial Resistance

Cited by 7 publications

References 66 publications

Global hotspots and emerging trends in 3D bioprinting research

Global hotspots and emerging trends in 3D bioprinting research

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Mechanical Properties Affect Primary T Cell Activation in 3D Bioprinted Hydrogels

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