Pulsed laser ablation of magnetic nanoparticles as a novel antibacterial strategy against gram positive bacteria

Morone, Maria Vittoria; Dell’Annunziata, Federica; Giugliano, Rosa; Chianese, Annalisa; Filippis, Anna De; Rinaldi, Luca; Gambardella, U.; Franci, Gianluigi; Galdiero, Massimiliano; Morone, A.

doi:10.1016/j.apsadv.2022.100213

Cited by 13 publications

(2 citation statements)

References 30 publications

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“…The target material was inserted in a quartz cuvette, and it was filled with 10 mL phosphate-buffered saline (PBS) 1×. During the PLAL process, an atmospheric pressure equal to 760 mm Hg, a temperature equal to 23 • C, and a pulse duration equal to 120 min were used [29].…”

Section: Methods For Production Of Agnpsmentioning

confidence: 99%

Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria

Morone,

Chianese,

Dell’Annunziata

et al. 2024

Microorganisms

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The spread of antibiotic-resistant bacteria and the rise of emerging and re-emerging viruses in recent years constitute significant public health problems. Therefore, it is necessary to develop new antimicrobial strategies to overcome these challenges. Herein, we describe an innovative method to synthesize ligand-free silver nanoparticles by Pulsed Laser Ablation in Liquid (PLAL-AgNPs). Thus produced, nanoparticles were characterized by total X-ray fluorescence, zeta potential analysis, transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate the nanoparticles’ cytotoxicity. Their potential was evaluated against the enveloped herpes simplex virus type 1 (HSV-1) and the naked poliovirus type 1 (PV-1) by plaque reduction assays and confirmed by real-time PCR and fluorescence microscopy, showing that nanoparticles interfered with the early stage of infection. Their action was also examined against different bacteria. We observed that the PLAL-AgNPs exerted a strong effect against both methicillin-resistant Staphylococcus aureus (S. aureus MRSA) and Escherichia coli (E. coli) producing extended-spectrum β-lactamase (ESBL). In detail, the PLAL-AgNPs exhibited a bacteriostatic action against S. aureus and a bactericidal activity against E. coli. Finally, we proved that the PLAL-AgNPs were able to inhibit/degrade the biofilm of S. aureus and E. coli.

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Section: Methods For Production Of Agnpsmentioning

confidence: 99%

Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria

Morone,

Chianese,

Dell’Annunziata

et al. 2024

Microorganisms

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“…The excess CV was removed and then solubilized with 98% ethanol for 40 min at room temperature under orbital shaking. The absorbance values at 570 nm were obtained using a microplate reader (Tecan, Männedorf, Swiss) [37].…”

Section: Biofilm Inhibition and Degradationmentioning

confidence: 99%

In Vitro Antibacterial and Anti-Inflammatory Activity of Arctostaphylos uva-ursi Leaf Extract against Cutibacterium acnes

Dell’Annunziata

Cometa²,

Marca

et al. 2022

Pharmaceutics

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Cutibacterium acnes (C. acnes) is the main causative agent of acne vulgaris. The study aims to evaluate the antimicrobial activity of a natural product, Arctostaphylos uva-ursi leaf extract, against C. acnes. Preliminary chemical–physical characterization of the extract was carried out by means of FT-IR, TGA and XPS analyses. Skin permeation kinetics of the extract conveyed by a toning lotion was studied in vitro by Franz diffusion cell, monitoring the permeated arbutin (as the target component of the extract) and the total phenols by HPLC and UV-visible spectrophotometry, respectively. Antimicrobial activity and time-killing assays were performed to evaluate the effects of Arctostaphylos uva-ursi leaf extract against planktonic C. acnes. The influence of different Arctostaphylos uva-ursi leaf extract concentrations on the biofilm biomass inhibition and degradation was evaluated by the crystal violet (CV) method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test was used to determine the viability of immortalized human keratinocytes (HaCaT) after exposure to Arctostaphylos uva-ursi leaf extract for 24 and 48 h. Levels of interleukin (IL)-1β, IL-6, IL-8 and tumour necrosis factor (TNF)-α were quantified after HaCaT cells cotreatment with Arctostaphylos uva-ursi leaf extract and heat-killed C. acnes. The minimum inhibitory concentration (MIC) which exerted a bacteriostatic action on 90% of planktonic C. acnes (MIC90) was 0.6 mg/mL. Furthermore, MIC and sub-MIC concentrations influenced the biofilm formation phases, recording a percentage of inhibition that exceeded 50 and 40% at 0.6 and 0.3 mg/mL. Arctostaphylos uva-ursi leaf extract disrupted biofilm biomass of 57 and 45% at the same concentrations mentioned above. Active Arctostaphylos uva-ursi leaf extract doses did not affect the viability of HaCaT cells. On the other hand, at 1.25 and 0.6 mg/mL, complete inhibition of the secretion of pro-inflammatory cytokines was recorded. Taken together, these results indicate that Arctostaphylos uva-ursi leaf extract could represent a natural product to counter the virulence of C. acnes, representing a new alternative therapeutic option for the treatment of acne vulgaris.

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Effect of Liquid and Laser Parameters on Fabrication of Nanoparticles via Pulsed Laser Ablation in Liquid with Their Applications: A Review

et al. 2023

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Pulsed laser ablation of magnetic nanoparticles as a novel antibacterial strategy against gram positive bacteria

Cited by 13 publications

References 30 publications

Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria

Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria

In Vitro Antibacterial and Anti-Inflammatory Activity of Arctostaphylos uva-ursi Leaf Extract against Cutibacterium acnes

Effect of Liquid and Laser Parameters on Fabrication of Nanoparticles via Pulsed Laser Ablation in Liquid with Their Applications: A Review

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