Pulsed electromagnetic fields (PEMF) attenuate changes in vertebral bone mass, architecture and strength in ovariectomized mice

Tao, Ling; Liang, Zhicheng; Li, Feijiang; Tang, Chi; Xie, Kangning; Wang, Pan; Xu, Dan; Shan, Shuai; Jiang, Maogang; Qian, Xu; Luo, Erping; Shen, Guanghao

doi:10.1016/j.bone.2017.12.008

Cited by 57 publications

(45 citation statements)

References 47 publications

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“…(34)(35)(36)(37)(38)(39)(40)(41) However, few researchers have investigated the Wnt ligands involved in the process, except for Wnt1 and Wnt3a. (42)(43)(44)(45)(46)(47) In the present study, we did not find increased expression of Wnt3a in the bone samples of rats and in vitro cultured osteoblasts treated by SEMFs, but found the significantly increased expression of Wnt10b. Wnt10b is one of the most extensively studied Wnt ligands acting through the canonical Wnt signaling that increases postnatal bone formation by enhancing osteoblast differentiation.…”

Section: Discussioncontrasting

confidence: 73%

“…There have been many clinical trials and experimental studies reporting that PEMFs modulate bone metabolism through the Wnt/β‐catenin pathway . However, few researchers have investigated the Wnt ligands involved in the process, except for Wnt1 and Wnt3a . In the present study, we did not find increased expression of Wnt3a in the bone samples of rats and in vitro cultured osteoblasts treated by SEMFs, but found the significantly increased expression of Wnt10b.…”

Section: Discussioncontrasting

confidence: 65%

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Sinusoidal Electromagnetic Fields Increase Peak Bone Mass in Rats by Activating Wnt10b/β-Catenin in Primary Cilia of Osteoblasts

Zhou

Gao

Zhu

et al. 2019

Journal of Bone and Mineral Research

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Extremely low‐frequency electromagnetic fields have been considered a potential candidate for the prevention and treatment of osteoporosis; however, their action mechanism and optimal magnetic flux density (intensity) parameter are still elusive. The present study found that 50‐Hz sinusoidal electromagnetic fields (SEMFs) at 1.8 mT increased the peak bone mass of young rats by increasing bone formation. Gene array expression studies with femoral bone samples showed that SEMFs increased the expression levels of collagen‐1α1 and Wnt10b, a critical ligand of the osteogenic Wnt/β‐catenin pathway. Consistently, SEMFs promoted osteogenic differentiation and maturation of rat calvarial osteoblasts (ROBs) in vitro through activating the Wnt10b/β‐catenin pathway. This osteogenesis‐promoting effect of SEMFs via Wnt10b/β‐catenin signaling was found to depend on the functional integrity of primary cilia in osteoblasts. When the primary cilia were abrogated by small interfering RNA (siRNA) targeting IFT88, the ability of SEMFs to promote the osteogenic differentiation of ROBs through activating Wnt10b/β‐catenin signaling was blocked. Although the knockdown of Wnt10b expression with RNA interference had no effect on primary cilia, it significantly suppressed the promoting effect of SEMFs on osteoblastic differentiation/maturation. Wnt10b was normally localized at the bases of primary cilia, but it disappeared (or was released) from the cilia upon SEMF treatment. Interestingly, primary cilia were elongated to different degrees by different intensities of 50‐Hz SEMFs, with the window effect observed at 1.8 mT, and the expression level of Wnt10b increased in accord with the lengths of primary cilia. These results indicate that 50‐Hz 1.8‐mT SEMFs increase the peak bone mass of growing rats by promoting osteogenic differentiation/maturation of osteoblasts, which is mediated, at least in part, by Wnt10b at the primary cilia and the subsequent activation of Wnt/β‐catenin signaling. © 2019 American Society for Bone and Mineral Research.

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Section: Discussioncontrasting

confidence: 73%

Section: Discussioncontrasting

confidence: 65%

Sinusoidal Electromagnetic Fields Increase Peak Bone Mass in Rats by Activating Wnt10b/β-Catenin in Primary Cilia of Osteoblasts

Zhou

Gao

Zhu

et al. 2019

Journal of Bone and Mineral Research

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“…Different types and parameters of magnetic fields may have different effects on bone cells, some of which are summarized in Table 4. Irrespective of whether SMFs or PEMFs are employed, the effects on bone regeneration may be related mainly to activation of Wnt/β-catenin (Zhou et al, 2012), RANK/RANKL/OPG (Lei et al, 2017(Lei et al, , 2018 or MAPK (mitogen-activated protein kinase) (Yong et al, 2016) signaling pathways. Moreover, bone tissue may be sensitive to low-frequency PEMFs, which may be associated with strong transmembrane potential changes and Ca 2+ influx.…”

Section: Biological Effects and Mechanisms Of Magnetic Materials On Bmentioning

confidence: 99%

Magnetic Materials in Promoting Bone Regeneration

et al. 2019

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Strategies to promote bone regeneration have been always the focus of investigations since the skeleton plays important roles like mechanical support, organ protection, and mineral homeostasis maintenance in the normal physiological activities of the human body. Magnetic fields have shown to be highly influential in the regeneration process, arousing tremendous interest in utilizing magnetic materials to enhance osteogenesis. In this work, we attempt a more comprehensive and detailed review of magnetic materials in promoting bone regeneration by including not only the mechanisms of bone regeneration, the history and basic concepts of magnetism, but also the types of magnetic materials as well as their influence parameters, designs and fabrication techniques with a focus on their usage in the field of bone regeneration like 3D printed scaffolds and implants. In addition, we provide some possible ideas on the synergistic action between magnetic and other materials on bone tissue. Finally, we propose the development trend of magnetic materials in the field of bone regeneration in the future. There is a huge demand for a more effective and less traumatic way to accelerate bone regeneration of the patients with diseases like fractures, tumors and osteoporosis that cause severe pains, bone loss, limb deformations, and restrictions of mobility. Magnetic materials have substantial potential to be manufactured into novel clinical applications with the ability to increase the bone regeneration efficiency.

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“…The use of EMFs to promote bone healing is supported by abundant preclinical literature that shows their action in rodent models of glucocorticoid‐ or ovariectomy‐induced osteoporosis [Zati et al, ; Bilotta et al, ; Chang and Chang, ; Jing et al, , ; Zhou et al, , , , b; Jiang et al, ; Lei et al, ], disuse osteoporosis [Shen and Zhao, ; Jing et al, ; Li et al, ], osteoporotic fractures [Androjna et al, ], osteoarthritis [Yang et al, ], diabetes‐associated bone architecture [Li et al, ], hyperthyroidism bone loss [Liu et al, ], and bone healing [Takano‐Yamamoto et al, ; Sarker et al, ; Landry et al, ; Fredericks et al, ; Midura et al, ; Taylor et al, ; Bilgin et al, ; Huegel et al, ].…”

Section: Therapeutic Effects Of Emfs On Bonementioning

confidence: 99%

The cellular effects of Pulsed Electromagnetic Fields on osteoblasts: A review

Galli

Pedrazzi

Guizzardi

2019

Bioelectromagnetics

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Electromagnetic fields (EMFs) have long been known to interact with living organisms and their cells and to bear the potential for therapeutic use. Among the most extensively investigated applications, the use of Pulsed EMFs (PEMFs) has proven effective to ameliorate bone healing in several studies, although the evidence is still inconclusive. This is due in part to our still‐poor understanding of the mechanisms by which PEMFs act on cells and affect their functions and to an ongoing lack of consensus on the most effective parameters for specific clinical applications. The present review has compared in vitro studies on PEMFs on different osteoblast models, which elucidate potential mechanisms of action for PEMFs, up to the most recent insights into the role of primary cilia, and highlight the critical issues underlying at least some of the inconsistent results in the available literature. Bioelectromagnetics. 2019;9999:XX–XX. © 2019 Bioelectromagnetics Society.

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Pulsed electromagnetic fields (PEMF) attenuate changes in vertebral bone mass, architecture and strength in ovariectomized mice

Cited by 57 publications

References 47 publications

Sinusoidal Electromagnetic Fields Increase Peak Bone Mass in Rats by Activating Wnt10b/β-Catenin in Primary Cilia of Osteoblasts

Sinusoidal Electromagnetic Fields Increase Peak Bone Mass in Rats by Activating Wnt10b/β-Catenin in Primary Cilia of Osteoblasts

Magnetic Materials in Promoting Bone Regeneration

The cellular effects of Pulsed Electromagnetic Fields on osteoblasts: A review

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