Pulsed electrochemical detection of sulfur-containing antibiotics following high performance liquid chromatography

LaCourse, William R.; Dasenbrock, Catherine O.

doi:10.1016/s0731-7085(98)00089-2

Cited by 55 publications

(26 citation statements)

References 15 publications

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“…Unlike common amperometric measurement, the applied potential waveform is an alternating potential between positive and negative values [27]. This cleans and reactivates the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Karuwan

Mantim

Chaisuwan

et al. 2006

Sensors

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This work presents the construction and application of boron-doped diamond (BDD) thin film electrode as sensor for the determination of three β-agonists, viz. salbutamol, terbutaline and clenbuterol. Although well-known as a chemically inert material, BDD film however shows fouling in detection of these compounds using fixedpotential mode amperometry. A suitable waveform for pulsed amperometric detection (PAD) was developed and used to determine the agonist compounds. It was seen that the developed PAD significantly refreshed the BDD surface for long-term detection in flow injection analysis. Linear working ranges were 0.5-100 µM, 1.0-100 µM and 0.5-50 µM for salbutamol, terbutaline and clenbuterol, respectively. The developed PAD-BDD system was applied to successfully determine salbutamol and terbutaline in commercial pharmaceutical products. The methods were validated with a capillary electrophoresis method.

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Section: Introductionmentioning

confidence: 99%

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Karuwan

Mantim

Chaisuwan

et al. 2006

Sensors

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“…This C18 column works at a pH between 2 and 8. Under such mildly acidic conditions, PED at am Au electrode has proven to be selective for sulfur-containing compounds [29][30][31][32][33][34]. In LaCourse's work, PED at pH 4.54 is specific to the detection of the sulfurcontaining compounds IPTG.…”

Section: Chromatographic Conditions For Ic-pad Analysismentioning

confidence: 99%

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

Wang¹,

Lin²

2017

Mod Chem Appl

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A novel method for the determination of isopropyl-β-D-thiogalactopyranoside (IPTG), a sulfur-containing compound, a pharmaceutical additive in commercial recombinant human growth hormone (rhGH), has been developed using pulsed electrochemical detection (PED), following ion Chromatographic separation. A Dionex-500 ion chromatograph coupled with an electrochemical detector was employed, equipped with a gold working electrode and an Acclaim 300 analytical C18 column, with a mobile phase consisting of sodium acetate (NaOAc) buffer (pH 5.45, 0.01 mol/L) and acetonitrile (ACN)(90/10, v/v). Upon optimization, IPTG was found to have a limit of detection of 1 ng/mL (0.1 μmol) with 25 uL injection volume. This method was successfully applied to the determination of IPTG in rhGH samples with the characteristics of simplicity, high sensitivity and good repeatability.

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“…It provides significant enhancement of sensitivity for compounds generally considered as non-electroactive for detection under constant applied potential and with poor optical properties for spectrophotometric detection (carbohydrates, amines, thiols) and macromolecules of biological importance (peptides, proteins). PIA is based on the capacity of Au to catalyse the oxidation of organic compounds [24]. The unsaturated d-electron orbitals present in the Au can bind and stabilise free-radical intermediates generated during electrochemical oxidation, promoting electron transfer from the oxidised analyte to the Au surface [25] hence allowing for the quantification of the target analyte.…”

Section: Pulse Amperometric Detectionmentioning

confidence: 99%

Review: The Application of Liquid Chromatography Electrochemical Detection for the Determination of Drugs of Abuse

Honeychurch

2016

Separations

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This review (4 tables, 88 references) describes current developments in the design and application of liquid chromatography electrochemical detection (LC ED) based approaches for the determination of drugs of abuse. Specific emphasis is placed on operating details and performance characteristics for selected applications. LC ED has been shown to be highly sensitive and specific as well being a more economic option. A wide range of abused substances have been determined using this approach, including: cannabinoids, ethanol, opiates, morphine, mushroom toxins, benzodiazepines and several legal highs. Reverse-phase liquid chromatography with either amperometric or coulometric determination has been the most commonly reported applications. However, coulometric arrays have been also reported. Detection limits in the ng/mL region have been reported for most target analytes.

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Pulsed electrochemical detection of sulfur-containing antibiotics following high performance liquid chromatography

Cited by 55 publications

References 15 publications

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Pulsed Amperometry for Anti-fouling of Boron-doped Diamond in Electroanalysis of β-Agonists: Application to Flow Injection for Pharmaceutical Analysis

Determination of IPTG in Recombinant Human Growth Hormone with Ion Chromatography and Pulsed Electrochemical Detection

Review: The Application of Liquid Chromatography Electrochemical Detection for the Determination of Drugs of Abuse

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